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An innovative approach to monitoring non-point source pollution at a field scale:online monitoring system for continuous cropping with a serial pipeline
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作者 peipei feng Gaofei YIN +7 位作者 Qingyi ZHU Tongyang LI Bin XI Xiaoyuan XU Huiqing JIAO Hongda WEN Lingling HUA Wenchao LI 《Frontiers of Agricultural Science and Engineering》 2025年第3期611-619,共9页
Non-point source(NPS)pollution has been the major cause of water quality degradation.However,there are still shortcomings in the current monitoring methods for NPS pollution,such as small monitoring range,error of mon... Non-point source(NPS)pollution has been the major cause of water quality degradation.However,there are still shortcomings in the current monitoring methods for NPS pollution,such as small monitoring range,error of monitoring data,time-consuming and laborious monitoring process.Although the established method,field experiment plots,was used effectively in the first and second national pollution source census in China.However,when the results obtained by monitoring experimental plots are extrapolated to a field or larger scale,there are considerable uncertainties because of the characteristics of large spatial and temporal variation of farmland.To optimize the farmland surface runoff monitoring methods,an online monitoring system for continuous cropping based on a serial pipeline was developed,which takes diversion trench,online flowmeter and dynamic acquisition device as the main body.Compared with the current farmland monitoring methods,this system can realize more precise automatic monitoring of water quantity and quality,and lower costs.This innovative method will provide greater confidence in the actual monitoring of NPS pollution from farmland and wider practical application.This new method could prove particularly valuable for the next national pollution source census in China. 展开更多
关键词 Farmland runoff online monitoring autocollection AGRICULTURE non-point source pollution
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rps12基因在裸子植物中的分子进化式样 被引量:4
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作者 平晶耀 冯佩沛 +3 位作者 郝静 栗锦烨 苏应娟 王艇 《科学通报》 EI CAS CSCD 北大核心 2021年第24期3182-3193,共12页
叶绿体rps12基因是所有叶绿体基因中最特殊的,由在基因组上相距甚远的两部分组成,含有3个外显子,经反式剪接作用编码核糖体小亚基S12蛋白.探究该基因的分子进化式样有助于理解该基因的进化过程及基因组特征.应用二代测序技术对南洋杉和... 叶绿体rps12基因是所有叶绿体基因中最特殊的,由在基因组上相距甚远的两部分组成,含有3个外显子,经反式剪接作用编码核糖体小亚基S12蛋白.探究该基因的分子进化式样有助于理解该基因的进化过程及基因组特征.应用二代测序技术对南洋杉和柏木的叶绿体基因组进行测序;共选取78种裸子植物和2种蕨类植物,在系统发育背景下结合最大似然法,对rps12基因的进化速率、选择压力和适应性进化进行分析.结果显示,外显子3发生独立的碱基插入事件,发生插入的序列受到了更强的负选择作用;外显子2~3位于反向重复区,它们的替换率显著低于外显子1, rps12编码序列受到更强的负选择作用;适应性进化分析没有检测到正选择位点.结果表明,在裸子植物的进化过程中rps12基因结构稳定,并保持着相对较低的进化速率且表现出高度的保守特性;这为反向重复区具有降低的替换率提供了更多的证据,同时也揭示了裸子植物中存在的进化速率异质性现象. 展开更多
关键词 裸子植物 rps12 反向重复区 进化速率 选择压力 适应性进化
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