Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophr...Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophrenia results in impaired social function,repeated hospital admissions,reduced quality of life and decreased life expectancy.Clinical diagnosis largely relies on subjective evidence,including self-reported experiences,and reported behavioural abnormalities followed by psychiatric evaluation.In addition,psychoses may occur along with other conditions,and the symptoms are often episodic and transient,posing a significant challenge to the precision of diagnosis.Therefore,objective,specific tests using biomarkers are urgently needed for differential diagnosis of schizophrenia in clinical practice.Aims We aimed to provide evidence-based and consensus-based recommendations,with a summary of laboratory measurements that could potentially be used as biomarkers for schizophrenia,and to discuss directions for future research.Methods We searched publications within the last 10 years with the following keywords:‘schizophrenia’,‘gene’,‘inflammation’,‘neurotransmitter’,‘protein marker’,‘gut microbiota’,‘pharmacogenomics’and‘biomarker’.A draft of the consensus was discussed and agreed on by all authors at a round table session.Results We summarised the characteristics of candidate diagnostic markers for schizophrenia,including genetic,inflammatory,neurotransmitter,peripheral protein,pharmacogenomic and gut microbiota markers.We also proposed a novel laboratory process for diagnosing schizophrenia in clinical practice based on the evidence summarised in this paper.Conclusions Further efforts are needed to identify schizophrenia-specific genetic and epigenetic markers for precise diagnosis,differential diagnosis and ethnicity-specific markers for the Chinese population.The development of novel laboratory techniques is making it possible to use these biomarkers clinically to diagnose disease.展开更多
Until very recently, gallium oxide(Ga_2O_3) has aroused more and more interests in the area of power electronics due to its ultra-wide bandgap of 4.5–4.8 eV, estimated critical field of 8 MV/cm and decent intrinsic e...Until very recently, gallium oxide(Ga_2O_3) has aroused more and more interests in the area of power electronics due to its ultra-wide bandgap of 4.5–4.8 eV, estimated critical field of 8 MV/cm and decent intrinsic electron mobility limit of250 cm2/(V·s), yielding a high Baliga's figures-of-merit(FOM) of more than 3000, which is several times higher than GaN and SiC.In addition to its excellent material properties, potential low-cost and large size substrate through melt-grown methodology also endows β-Ga_2O_3 more potential for future low-cost power devices. This article focuses on reviewing the most recent advances ofβ-Ga_2O_3 based power devices. It will be starting with a brief introduction to the material properties of β-Ga_2O_3 and then the growth techniques of its native substrate, followed by the thin film epitaxial growth. The performance of state-of-art β-Ga_2O_3 devices, including diodes and FETs are fully discussed and compared. Finally, potential solutions to the challenges of β-Ga_2O_3 are also discussed and explored.展开更多
We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome manychallenges hindering the development of scRNA-seq in bacteria. BacDrop can be applied to thousands to m...We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome manychallenges hindering the development of scRNA-seq in bacteria. BacDrop can be applied to thousands to millions of cells fromboth gram-negative and gram-positive species. It features universal ribosomal RNA depletion and combinatorial barcodes thatenable multiplexing and massively parallel sequencing. We applied BacDrop to study Klebsiella pneumoniae clinical isolates andto elucidate their heterogeneous responses to antibiotic stress. In an unperturbed population presumed to be homogeneous, wefound within-population heterogeneity largely driven by the expression of mobile genetic elements that promote the evolution ofantibiotic resistance. Under antibiotic perturbation, BacDrop revealed transcriptionally distinct subpopulations associated withdifferent phenotypic outcomes including antibiotic persistence. BacDrop thus can capture cellular states that cannot be detectedby bulk RNA-seq, which will unlock new microbiological insights into bacterial responses to perturbations and larger bacterialcommunities such as the microbiome.展开更多
The traditional similar code detection approaches are limited in detecting semantically similar codes, impeding their applications in practice. In this paper, we have improved the traditional metrics-based approach as...The traditional similar code detection approaches are limited in detecting semantically similar codes, impeding their applications in practice. In this paper, we have improved the traditional metrics-based approach as well as the graph- based approach and presented a metrics-based and graph- based combined approach. First, source codes are represented as augmented system dependence graphs. Then, metrics- based candidate similar code extraction is performed to filter out most of the dissimilar code pairs so as to lower the computational complexity. After that, code normalization is performed on the candidate similar codes to remove code variations so as to detect similar code at the semantic level. Finally, program matching is performed on the normalized control dependence trees to output semantically similar codes. Experiment results show that our approach can detect similar codes with code variations, and it can be applied to large software.展开更多
Regulatory T cells(Treg cells or Tregs),a subset of CD4+T cells with immunosuppressive properties,are essential for immune homeostasis and self-tolerance.Characterized by their immunosuppressive capabilities and relia...Regulatory T cells(Treg cells or Tregs),a subset of CD4+T cells with immunosuppressive properties,are essential for immune homeostasis and self-tolerance.Characterized by their immunosuppressive capabilities and reliance on the transcription factor Foxp3(Forkhead box protein P3),Tregs employ multiple mechanisms,including cytokine secretion,metabolic control,and cell contact inhibition,to restrain excessive immune activation to prevent autoimmunity while maintaining tissue repair processes.However,dysregulation in their frequency or function-whether deficiency or hyperactivity-is implicated in diverse pathologies,spanning autoimmune disorders,cancer progression,transplant rejection,and emerging associations with neurological and cardiovascular diseases.Thus,Treg-targeted strategies represent a promising approach for restoring immune balance under various conditions.This review synthesizes current knowledge on Treg biology,from their discovery and definition of markers to their new regulatory mechanisms.We further explore the roles of Tregs across diseases,emphasizing their context-dependent therapeutic potential.Strategies to deplete or inhibit Tregs in cancer immunotherapy contrast with approaches to expand or stabilize their function in autoimmunity and transplantation.However,challenges persist,including achieving tissue-specific targeting,ensuring the functional stability of engineered Tregs,and minimizing off-target effects.By integrating mechanistic insights with translational innovations,this review provides a roadmap for advancing Treg-based therapies,ultimately aiming to restore immune equilibrium in a disease-specific manner.展开更多
Hierarchical TiO2 photocatalysts with a one-dimensional heterojunction were synthesized via a facile template-free hydrothermal method. The TiO2 photo- catalysts were flower-like microspheres with a 3 μm diameter. Th...Hierarchical TiO2 photocatalysts with a one-dimensional heterojunction were synthesized via a facile template-free hydrothermal method. The TiO2 photo- catalysts were flower-like microspheres with a 3 μm diameter. The base structure of the flower-like microspheres was a uniform nanowire with a 10 nm diameter. Anatase films were evenly coated onto the surface of the rutile TiO2 nanowires to form a one-dimensional core--shell base structure. This kind of one-dimensional heterojunction is conducive to the separation of charge carriers. In addition, the hierarchical TiO2 microspheres possessed a good mesoporous structure with a high specific surface area of 260 m2/g. Thus, the light scattering and utilization efficiency were improved in this structure. The photocatalysts exhibited better performance in both photocatalytic oxidation and reduction reactions. Moreover, the novel TiO2 photocatalysts displayed excellent stability in these reactions. This kind of hierarchical TiO2 structure has never been reported in the literature. The hierarchical structure and one-dimensional heterojunction were vital to the increase in quantum efficiency. Therefore, these hierarchical TiO2 photocatalysts have potential applications in the environmental and energy fields, such as in photocatalytic degradation, hydrogen production, Li-ion batteries, and dye-sensitized solar cells.展开更多
基金This study was funded by Shanghai Natural Science fund Project under grant(20ZR1447700)The National Key Development Plan for Precision Medicine Research(2017YFC0910002)+4 种基金the hospital-level project of Shanghai Mental Health Center under grant(2020-YJ05)Special Fund for Clinical Research of Wu Jieping Medical Foundation(320675015232)the Hospital level project of Shanghai Mental Health Center(2016-YJ-09)Chinese medicine research project of Shanghai Health and Family Planning Commission(2018LP024)the characteristic discipline construction project of Shanghai Mental Health Center(2017-TSXK-07),China Public Health Alliance(first batch)(GWLM202014).
文摘Background Schizophrenia is a serious mental illness affecting approximately 20 million individuals globally.Both genetic and environmental factors contribute to the illness.If left undiagnosed and untreated,schizophrenia results in impaired social function,repeated hospital admissions,reduced quality of life and decreased life expectancy.Clinical diagnosis largely relies on subjective evidence,including self-reported experiences,and reported behavioural abnormalities followed by psychiatric evaluation.In addition,psychoses may occur along with other conditions,and the symptoms are often episodic and transient,posing a significant challenge to the precision of diagnosis.Therefore,objective,specific tests using biomarkers are urgently needed for differential diagnosis of schizophrenia in clinical practice.Aims We aimed to provide evidence-based and consensus-based recommendations,with a summary of laboratory measurements that could potentially be used as biomarkers for schizophrenia,and to discuss directions for future research.Methods We searched publications within the last 10 years with the following keywords:‘schizophrenia’,‘gene’,‘inflammation’,‘neurotransmitter’,‘protein marker’,‘gut microbiota’,‘pharmacogenomics’and‘biomarker’.A draft of the consensus was discussed and agreed on by all authors at a round table session.Results We summarised the characteristics of candidate diagnostic markers for schizophrenia,including genetic,inflammatory,neurotransmitter,peripheral protein,pharmacogenomic and gut microbiota markers.We also proposed a novel laboratory process for diagnosing schizophrenia in clinical practice based on the evidence summarised in this paper.Conclusions Further efforts are needed to identify schizophrenia-specific genetic and epigenetic markers for precise diagnosis,differential diagnosis and ethnicity-specific markers for the Chinese population.The development of novel laboratory techniques is making it possible to use these biomarkers clinically to diagnose disease.
文摘Until very recently, gallium oxide(Ga_2O_3) has aroused more and more interests in the area of power electronics due to its ultra-wide bandgap of 4.5–4.8 eV, estimated critical field of 8 MV/cm and decent intrinsic electron mobility limit of250 cm2/(V·s), yielding a high Baliga's figures-of-merit(FOM) of more than 3000, which is several times higher than GaN and SiC.In addition to its excellent material properties, potential low-cost and large size substrate through melt-grown methodology also endows β-Ga_2O_3 more potential for future low-cost power devices. This article focuses on reviewing the most recent advances ofβ-Ga_2O_3 based power devices. It will be starting with a brief introduction to the material properties of β-Ga_2O_3 and then the growth techniques of its native substrate, followed by the thin film epitaxial growth. The performance of state-of-art β-Ga_2O_3 devices, including diodes and FETs are fully discussed and compared. Finally, potential solutions to the challenges of β-Ga_2O_3 are also discussed and explored.
文摘We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome manychallenges hindering the development of scRNA-seq in bacteria. BacDrop can be applied to thousands to millions of cells fromboth gram-negative and gram-positive species. It features universal ribosomal RNA depletion and combinatorial barcodes thatenable multiplexing and massively parallel sequencing. We applied BacDrop to study Klebsiella pneumoniae clinical isolates andto elucidate their heterogeneous responses to antibiotic stress. In an unperturbed population presumed to be homogeneous, wefound within-population heterogeneity largely driven by the expression of mobile genetic elements that promote the evolution ofantibiotic resistance. Under antibiotic perturbation, BacDrop revealed transcriptionally distinct subpopulations associated withdifferent phenotypic outcomes including antibiotic persistence. BacDrop thus can capture cellular states that cannot be detectedby bulk RNA-seq, which will unlock new microbiological insights into bacterial responses to perturbations and larger bacterialcommunities such as the microbiome.
基金Acknowledgements This work was supported by the National Natural Science Foundation of China （Grant Nos. 61202092 and 61173021）, the Research Fund for the Doctoral Program of Higher Education of China （20112302120052）, Research Fund for the Innovative Scholars of Harbin （RC2013QN010001）, and Young Colleger Academic Backbone Project of Heilongjiang.
文摘The traditional similar code detection approaches are limited in detecting semantically similar codes, impeding their applications in practice. In this paper, we have improved the traditional metrics-based approach as well as the graph- based approach and presented a metrics-based and graph- based combined approach. First, source codes are represented as augmented system dependence graphs. Then, metrics- based candidate similar code extraction is performed to filter out most of the dissimilar code pairs so as to lower the computational complexity. After that, code normalization is performed on the candidate similar codes to remove code variations so as to detect similar code at the semantic level. Finally, program matching is performed on the normalized control dependence trees to output semantically similar codes. Experiment results show that our approach can detect similar codes with code variations, and it can be applied to large software.
基金supported by the National Natural Science Foundation of China(Grant no.82203445,82273293)the Shanghai Municipal Health Commission Health Industry Clinical Research Project(20224Y0120)+1 种基金the“Phoenix Introduction Plan”Talent Startover Project of Tangdu Hospital,Fourth Military Medical University(grant number 2022YFJH001)the Project of Shanghai Mental Health Center(grant number 2020-YJ05).
文摘Regulatory T cells(Treg cells or Tregs),a subset of CD4+T cells with immunosuppressive properties,are essential for immune homeostasis and self-tolerance.Characterized by their immunosuppressive capabilities and reliance on the transcription factor Foxp3(Forkhead box protein P3),Tregs employ multiple mechanisms,including cytokine secretion,metabolic control,and cell contact inhibition,to restrain excessive immune activation to prevent autoimmunity while maintaining tissue repair processes.However,dysregulation in their frequency or function-whether deficiency or hyperactivity-is implicated in diverse pathologies,spanning autoimmune disorders,cancer progression,transplant rejection,and emerging associations with neurological and cardiovascular diseases.Thus,Treg-targeted strategies represent a promising approach for restoring immune balance under various conditions.This review synthesizes current knowledge on Treg biology,from their discovery and definition of markers to their new regulatory mechanisms.We further explore the roles of Tregs across diseases,emphasizing their context-dependent therapeutic potential.Strategies to deplete or inhibit Tregs in cancer immunotherapy contrast with approaches to expand or stabilize their function in autoimmunity and transplantation.However,challenges persist,including achieving tissue-specific targeting,ensuring the functional stability of engineered Tregs,and minimizing off-target effects.By integrating mechanistic insights with translational innovations,this review provides a roadmap for advancing Treg-based therapies,ultimately aiming to restore immune equilibrium in a disease-specific manner.
文摘Hierarchical TiO2 photocatalysts with a one-dimensional heterojunction were synthesized via a facile template-free hydrothermal method. The TiO2 photo- catalysts were flower-like microspheres with a 3 μm diameter. The base structure of the flower-like microspheres was a uniform nanowire with a 10 nm diameter. Anatase films were evenly coated onto the surface of the rutile TiO2 nanowires to form a one-dimensional core--shell base structure. This kind of one-dimensional heterojunction is conducive to the separation of charge carriers. In addition, the hierarchical TiO2 microspheres possessed a good mesoporous structure with a high specific surface area of 260 m2/g. Thus, the light scattering and utilization efficiency were improved in this structure. The photocatalysts exhibited better performance in both photocatalytic oxidation and reduction reactions. Moreover, the novel TiO2 photocatalysts displayed excellent stability in these reactions. This kind of hierarchical TiO2 structure has never been reported in the literature. The hierarchical structure and one-dimensional heterojunction were vital to the increase in quantum efficiency. Therefore, these hierarchical TiO2 photocatalysts have potential applications in the environmental and energy fields, such as in photocatalytic degradation, hydrogen production, Li-ion batteries, and dye-sensitized solar cells.
