Pressure overload–induced cardiac hypertrophy is a common cause of heart failure(HF),and emerging evidence suggests that excessive oxidized lipids have a detrimental effect on cardiomyocytes.However,the key regulator...Pressure overload–induced cardiac hypertrophy is a common cause of heart failure(HF),and emerging evidence suggests that excessive oxidized lipids have a detrimental effect on cardiomyocytes.However,the key regulator of lipid toxicity in cardiomyocytes during this pathological process remains unknown.Here,we used lipidomics profiling and RNA-seq analysis and found that phosphatidylethanolamines(PEs)and Acsl4 expression are significantly increased in mice with transverse aortic constriction(TAC)–induced HF compared to sham-operated mice.In addition,we found that overexpressing Acsl4 in cardiomyocytes exacerbates pressure overload‒induced cardiac dysfunction via ferroptosis.Notably,both pharmacological inhibition and genetic deletion of Acsl4 significantly reduced left ventricular chamber size and improved cardiac function in mice with TAC-induced HF.Moreover,silencing Acsl4 expression in cultured neonatal rat ventricular myocytes was sufficient to inhibit hypertrophic stimulus‒induced cell growth.Mechanistically,we found that Acsl4-dependent ferroptosis activates the pyroptotic signaling pathway,which leads to increased production of the proinflammatory cytokine IL-1β,and neutralizing IL-1βimproved cardiac function in Acsl4 transgenic mice following TAC.These results indicate that ACSL4 plays an essential role in the heart during pressure overload‒induced cardiac remodeling via ferroptosis-induced pyroptotic signaling.Together,these findings provide compelling evidence that targeting the ACSL4-ferroptosis-pyroptotic signaling cascade may provide a promising therapeutic strategy for preventing heart failure.展开更多
Heart disease is the leading cause of mortality worldwide.Due to the limited proliferation rate of mature cardiomyocytes,adult mammalian hearts are unable to regenerate damaged cardiac muscle following injury.Instead,...Heart disease is the leading cause of mortality worldwide.Due to the limited proliferation rate of mature cardiomyocytes,adult mammalian hearts are unable to regenerate damaged cardiac muscle following injury.Instead,injured area is replaced by fibrotic scar tissue,which may lead to irreversible cardiac remodeling and organ failure.In contrast,adult zebrafish and neonatal mammalian possess the capacity for heart regeneration and have been widely used as experimental models.Recent studies have shown that multiple types of cells within the heart can respond to injury with the activation of distinct signaling pathways.Determining the specific contributions of each cell type is essential for our understanding of the regeneration network organization throughout the heart.In this review,we provide an overview of the distinct functions and coordinated cell behaviors of several major cell types including cardiomyocytes,endocardial cells,epicardial cells,fibroblasts,and immune cells.The topic focuses on their specific responses and cellular plasticity after injury,and potential therapeutic applications.展开更多
基金National Natural Science Foundation of China(81900232 to X.B.,32330047 and 31930057 to F.W.,82471593 to J.M.,81800706 to X.L.)Natural Science Foundation of Zhejiang Province(LQ19H020011 to X.B.)Chinese Postdoctoral Science Foundation(2019M652118 to X.B.).
文摘Pressure overload–induced cardiac hypertrophy is a common cause of heart failure(HF),and emerging evidence suggests that excessive oxidized lipids have a detrimental effect on cardiomyocytes.However,the key regulator of lipid toxicity in cardiomyocytes during this pathological process remains unknown.Here,we used lipidomics profiling and RNA-seq analysis and found that phosphatidylethanolamines(PEs)and Acsl4 expression are significantly increased in mice with transverse aortic constriction(TAC)–induced HF compared to sham-operated mice.In addition,we found that overexpressing Acsl4 in cardiomyocytes exacerbates pressure overload‒induced cardiac dysfunction via ferroptosis.Notably,both pharmacological inhibition and genetic deletion of Acsl4 significantly reduced left ventricular chamber size and improved cardiac function in mice with TAC-induced HF.Moreover,silencing Acsl4 expression in cultured neonatal rat ventricular myocytes was sufficient to inhibit hypertrophic stimulus‒induced cell growth.Mechanistically,we found that Acsl4-dependent ferroptosis activates the pyroptotic signaling pathway,which leads to increased production of the proinflammatory cytokine IL-1β,and neutralizing IL-1βimproved cardiac function in Acsl4 transgenic mice following TAC.These results indicate that ACSL4 plays an essential role in the heart during pressure overload‒induced cardiac remodeling via ferroptosis-induced pyroptotic signaling.Together,these findings provide compelling evidence that targeting the ACSL4-ferroptosis-pyroptotic signaling cascade may provide a promising therapeutic strategy for preventing heart failure.
基金The research in the Han’s lab is supported by the National Natural Science Foundation of China(31871462)the National Key R&D Program of China(2018YFA0800501,2018YFC1004801)。
文摘Heart disease is the leading cause of mortality worldwide.Due to the limited proliferation rate of mature cardiomyocytes,adult mammalian hearts are unable to regenerate damaged cardiac muscle following injury.Instead,injured area is replaced by fibrotic scar tissue,which may lead to irreversible cardiac remodeling and organ failure.In contrast,adult zebrafish and neonatal mammalian possess the capacity for heart regeneration and have been widely used as experimental models.Recent studies have shown that multiple types of cells within the heart can respond to injury with the activation of distinct signaling pathways.Determining the specific contributions of each cell type is essential for our understanding of the regeneration network organization throughout the heart.In this review,we provide an overview of the distinct functions and coordinated cell behaviors of several major cell types including cardiomyocytes,endocardial cells,epicardial cells,fibroblasts,and immune cells.The topic focuses on their specific responses and cellular plasticity after injury,and potential therapeutic applications.
