Skeletal stem/progenitor cells(SSPCs)are tissue-specific stem/progenitor cells localized within skeletons and contribute to bone development,homeostasis,and regeneration.However,the heterogeneity of SSPC populations i...Skeletal stem/progenitor cells(SSPCs)are tissue-specific stem/progenitor cells localized within skeletons and contribute to bone development,homeostasis,and regeneration.However,the heterogeneity of SSPC populations in mouse long bones and their respective regenerative capacity remain to be further clarified.In this study,we perform integrated analysis using single-cell RNA sequencing(scRNA-seq)datasets of mouse hindlimb buds,postnatal long bones,and fractured long bones.Our analyses reveal the heterogeneity of osteochondrogenic lineage cells and recapitulate the developmental trajectories during mouse long bone growth.In addition,we identify a novel Cd168þSSPC population with highly replicating capacity and osteochondrogenic potential in embryonic and postnatal long bones.Moreover,the Cd168þSSPCs can contribute to newly formed skeletal tissues during fracture healing.Furthermore,the results of multicolor immunofluorescence show that Cd168þSSPCs reside in the superficial zone of articular cartilage as well as in growth plates of postnatal mouse long bones.In summary,we identify a novel Cd168þSSPC population with regenerative potential in mouse long bones,which adds to the knowledge of the tissuespecific stem cells in skeletons.展开更多
We report on an injection seeded 1 kHz single frequency pulsed Nd:YAG ring laser with pulse energy of 5.2 mJ and pulse width of 9.9 ns.The ramp-fire technique is used to maintain single frequency operation and the cav...We report on an injection seeded 1 kHz single frequency pulsed Nd:YAG ring laser with pulse energy of 5.2 mJ and pulse width of 9.9 ns.The ramp-fire technique is used to maintain single frequency operation and the cavity length is modulated by an intracavity RbTiOPO4(RTP)crystal.The frequency stability(rms)of the output pulse is 1.99 MHz over 1 min and the linewidth is 64 MHz.展开更多
BACKGROUND Endoscopic resection of giant gastric leiomyomas,particularly in the fundus and cardia regions,is infrequently documented and presents a significant challenge for endoscopic surgery.CASE SUMMARY Herein,a ca...BACKGROUND Endoscopic resection of giant gastric leiomyomas,particularly in the fundus and cardia regions,is infrequently documented and presents a significant challenge for endoscopic surgery.CASE SUMMARY Herein,a case of a 59-year-old woman with a giant gastric leiomyoma was reported.The patient presented to the department of hepatological surgery with a complaint of right upper abdominal pain for one month and worsening for one week.The patient was diagnosed as gastric submucosal tumor(SMT),gallstone,and cholecystitis combined with computed tomography and gastroendoscopy prior to operation.Upon admission,following a multi-disciplinary treatment discussion,it was determined that the patient would undergo a laparoscopic cholecystectomy and endoscopic resection of gastric SMT.It took 3 hours to completely resect the lesion by Endoscopic submucosal excavation and endoscopic fullthickness resection,and about 3 hours to suture the wound and take out the lesion.The lesion,ginger-shaped and measuring 8 cm×5 cm,led to transient peritonitis post-surgery.With no cardiac complications,the patient was discharged one week after surgery.CONCLUSION Endoscopic resection of a giant leiomyoma in the cardiac fundus is feasible and suitable for skilled endoscopists.展开更多
Skeletal stem cells(SSC)have gained attentions as candidates for the treatment of osteoarthritis due to their osteochondrogenic capacity.However,the immunomodulatory properties of SSC,especially under delivery operati...Skeletal stem cells(SSC)have gained attentions as candidates for the treatment of osteoarthritis due to their osteochondrogenic capacity.However,the immunomodulatory properties of SSC,especially under delivery operations,have been largely ignored.In the study,we found that Pdpn+and Grem1+SSC subpopulations owned immunoregulatory potential,and the single-cell RNA sequencing(scRNA-seq)data suggested that the mechanical activation of microgel carriers on SSC induced the generation of Pdpn+Grem1+Ptgs2+SSC subpopulation,which was potent at suppressing macrophage inflammation.The microgel carriers promoted the YAP nuclear translocation,and the activated YAP protein was necessary for the increased expression of Ptgs2 and PGE2 in microgels-delivered SSC,which further suppressed the expression of TNF-ɑ,IL-1βand promoted the expression of IL-10 in macrophages.SSC delivered with microgels yielded better preventive effects on articular lesions and macrophage activation in osteoarthritic rats than SSC without microgels.Chemically blocking the YAP and Ptgs2 in microgels-delivered SSC partially abolished the enhanced protection on articular tissues and suppression on osteoarthritic macrophages.Moreover,microgel carriers significantly prolonged SSC retention time in vivo without increasing SSC implanting into osteoarthritic joints.Together,our study demonstrated that microgel carriers enhanced SSC reprogramming towards immunomodulatory phenotype to regulate macrophage phenotype transformation for effectively osteoarthritic therapy by promoting YAP protein translocation into nucleus.The study not only complement and perfect the immunological mechanisms of SSC-based therapy at the single-cell level,but also provide new insight for microgel carriers in stem cell-based therapy.展开更多
基金supported by the National Key R&D Program of China(2022YFA1104100,2022YFA1103500)the National Natural Sciences Grants China(82172388,82372373,81871771)the Beijing Natural Sciences Foundation(7222123,L212065).
文摘Skeletal stem/progenitor cells(SSPCs)are tissue-specific stem/progenitor cells localized within skeletons and contribute to bone development,homeostasis,and regeneration.However,the heterogeneity of SSPC populations in mouse long bones and their respective regenerative capacity remain to be further clarified.In this study,we perform integrated analysis using single-cell RNA sequencing(scRNA-seq)datasets of mouse hindlimb buds,postnatal long bones,and fractured long bones.Our analyses reveal the heterogeneity of osteochondrogenic lineage cells and recapitulate the developmental trajectories during mouse long bone growth.In addition,we identify a novel Cd168þSSPC population with highly replicating capacity and osteochondrogenic potential in embryonic and postnatal long bones.Moreover,the Cd168þSSPCs can contribute to newly formed skeletal tissues during fracture healing.Furthermore,the results of multicolor immunofluorescence show that Cd168þSSPCs reside in the superficial zone of articular cartilage as well as in growth plates of postnatal mouse long bones.In summary,we identify a novel Cd168þSSPC population with regenerative potential in mouse long bones,which adds to the knowledge of the tissuespecific stem cells in skeletons.
基金Supported by the National Key Research and Development Program of China under Grant No 2017YFB1104500the National Natural Science Foundation of China under Grant No 61875100
文摘We report on an injection seeded 1 kHz single frequency pulsed Nd:YAG ring laser with pulse energy of 5.2 mJ and pulse width of 9.9 ns.The ramp-fire technique is used to maintain single frequency operation and the cavity length is modulated by an intracavity RbTiOPO4(RTP)crystal.The frequency stability(rms)of the output pulse is 1.99 MHz over 1 min and the linewidth is 64 MHz.
文摘BACKGROUND Endoscopic resection of giant gastric leiomyomas,particularly in the fundus and cardia regions,is infrequently documented and presents a significant challenge for endoscopic surgery.CASE SUMMARY Herein,a case of a 59-year-old woman with a giant gastric leiomyoma was reported.The patient presented to the department of hepatological surgery with a complaint of right upper abdominal pain for one month and worsening for one week.The patient was diagnosed as gastric submucosal tumor(SMT),gallstone,and cholecystitis combined with computed tomography and gastroendoscopy prior to operation.Upon admission,following a multi-disciplinary treatment discussion,it was determined that the patient would undergo a laparoscopic cholecystectomy and endoscopic resection of gastric SMT.It took 3 hours to completely resect the lesion by Endoscopic submucosal excavation and endoscopic fullthickness resection,and about 3 hours to suture the wound and take out the lesion.The lesion,ginger-shaped and measuring 8 cm×5 cm,led to transient peritonitis post-surgery.With no cardiac complications,the patient was discharged one week after surgery.CONCLUSION Endoscopic resection of a giant leiomyoma in the cardiac fundus is feasible and suitable for skilled endoscopists.
基金supported by the National Key R&D Program of China(Nos.2022YFA1104100,2022YFA1103500,2022YFB3804400)the National Natural Sciences Grants China(Nos.82172388,82372373,81871771,52373128,52273121)+1 种基金the Beijing Natural Sciences Foundation(Nos.7222123,L212065,7222011)the Beijing Municipal Health Commission(Nos.BMHC-2021-6,BJRITO-RDP-2023).
文摘Skeletal stem cells(SSC)have gained attentions as candidates for the treatment of osteoarthritis due to their osteochondrogenic capacity.However,the immunomodulatory properties of SSC,especially under delivery operations,have been largely ignored.In the study,we found that Pdpn+and Grem1+SSC subpopulations owned immunoregulatory potential,and the single-cell RNA sequencing(scRNA-seq)data suggested that the mechanical activation of microgel carriers on SSC induced the generation of Pdpn+Grem1+Ptgs2+SSC subpopulation,which was potent at suppressing macrophage inflammation.The microgel carriers promoted the YAP nuclear translocation,and the activated YAP protein was necessary for the increased expression of Ptgs2 and PGE2 in microgels-delivered SSC,which further suppressed the expression of TNF-ɑ,IL-1βand promoted the expression of IL-10 in macrophages.SSC delivered with microgels yielded better preventive effects on articular lesions and macrophage activation in osteoarthritic rats than SSC without microgels.Chemically blocking the YAP and Ptgs2 in microgels-delivered SSC partially abolished the enhanced protection on articular tissues and suppression on osteoarthritic macrophages.Moreover,microgel carriers significantly prolonged SSC retention time in vivo without increasing SSC implanting into osteoarthritic joints.Together,our study demonstrated that microgel carriers enhanced SSC reprogramming towards immunomodulatory phenotype to regulate macrophage phenotype transformation for effectively osteoarthritic therapy by promoting YAP protein translocation into nucleus.The study not only complement and perfect the immunological mechanisms of SSC-based therapy at the single-cell level,but also provide new insight for microgel carriers in stem cell-based therapy.
