The core components of abscisic acid(ABA)signaling—PYR/PYL/RCAR ABA receptors,coreceptor clade A protein phosphatases 2C(PP2CAs),and SnRK2 kinases—are essential for mediating plant responses to ABA.Mutants impaired ...The core components of abscisic acid(ABA)signaling—PYR/PYL/RCAR ABA receptors,coreceptor clade A protein phosphatases 2C(PP2CAs),and SnRK2 kinases—are essential for mediating plant responses to ABA.Mutants impaired in these components exhibit either strong ABA-insensitive(pyl or snrk2 mutants)or ABA-hypersensitive(pp2c mutants)phenotypes.The recent discovery of RAF-like kinases as upstream activators of SnRK2s has added another regulatory layer to ABA signaling(Pizzio and Rodriguez,2022).展开更多
The bZIP transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)is a master regulator of seed germination and post-germinative growth in response to abscisic acid(ABA),but the detailed molecularmechanism by which it rep...The bZIP transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)is a master regulator of seed germination and post-germinative growth in response to abscisic acid(ABA),but the detailed molecularmechanism by which it represses plant growth remains unclear.In this study,we used proximity labeling to map the neighboring proteome of ABI5 and identified FCS-LIKE ZINC FINGER PROTEIN 13(FLZ13)as a novel ABI5 interaction partner.Phenotypic analysis of flz13 mutants and FLZ13-overexpressing lines demonstrated that FLZ13 acts as a positive regulator of ABA signaling.Transcriptomic analysis revealed that both FLZ13 and ABI5 downregulate the expression of ABA-repressed and growth-related genes involved in chlorophyll biosynthesis,photosynthesis,and cell wall organization,thereby repressing seed germination and seedling establishment in response to ABA.Further genetic analysis showed that FLZ13 and ABI5 function together to regulate seed germination.Collectively,our findings reveal a previously uncharacterized transcriptional regulatorymechanismby which ABA mediates inhibition of seed germination and seedling establishment.展开更多
Plants must adapt to unfavorable growth conditions such as drought,salinity,or extreme temperature and,in addition,need the flexibility to resume growth when conditions become favorable.Sensing of extracellular enviro...Plants must adapt to unfavorable growth conditions such as drought,salinity,or extreme temperature and,in addition,need the flexibility to resume growth when conditions become favorable.Sensing of extracellular environmental stress and signal transduction rely on pathways that in many cases use phosphorylation of target proteins to amplify and transmit the extracellular signal.展开更多
Crop yield is at increasing risk due to water scarcity and climate change.Agrochemicals can activate hor-mone receptors to regulate transpiration and modulate transcription and address water deficits.Structure-guided ...Crop yield is at increasing risk due to water scarcity and climate change.Agrochemicals can activate hor-mone receptors to regulate transpiration and modulate transcription and address water deficits.Structure-guided optimization of multiple abscisic acid(ABA)receptor-agonist interactions is necessary to activate theentirePYRABACTINRESISTANCE1(PYR1)/PYR1-LIKE(PYL)/REGULATORYCOMPONENTSOFABA RECEPTORS(RCAR)receptor family.The new agonist iCB,produced through scaffold-merging led by X-ray structure,activates subfamilies Il and Ill at low-nM concentrations and subfamily I receptors at higher-nM concentrations.Structural analysis of opabactin and icB ternary complexes reveals selectivity-determining residues,making the PYL1/PYL4/PYL8 subfamilies sensitive to specific agonists and highlighting the differential sensitivity of receptor subfamilies to agonists across plant species.icB may activate mosteudicots'PYL8-like receptors,incontrastto opabactin,dueto limited steric constraints.This enables iCB to activate PYL8-like receptors with a bulkier Leu residue in the 3'tunnel,such as AtPYL8,SIPYL8,and VviPYL8.In contrast,opabactin activation is limited to receptors with Val at this position,for example,TaPYL8.Therefore,iCBextends its action to moreABAreceptors than CB,iSBo9,andopabactin,exhibits higher affinity than ABA for dimeric receptors,and can protect tomato plants against drought.In addition to regulating stomatal conductance and lowering water consumption,icB protects photosystem Il and improves photosynthesis following prolonged water deficit.Moreover,icB induces an ABA-like tran-scriptional response,upregulates the osmolyte synthesis,and can be hyperpotentiated when combined with the expression of a customized receptor.Our results provide structural insightsfor optimizing agonist designand aiding plants in managing water deficits.展开更多
基金supported by MCIU with funding from the European Union NextGenerationEU(PRTR-C17.11)and Generalitat Valenciana.No conflict of interest declared。
文摘The core components of abscisic acid(ABA)signaling—PYR/PYL/RCAR ABA receptors,coreceptor clade A protein phosphatases 2C(PP2CAs),and SnRK2 kinases—are essential for mediating plant responses to ABA.Mutants impaired in these components exhibit either strong ABA-insensitive(pyl or snrk2 mutants)or ABA-hypersensitive(pp2c mutants)phenotypes.The recent discovery of RAF-like kinases as upstream activators of SnRK2s has added another regulatory layer to ABA signaling(Pizzio and Rodriguez,2022).
基金supported by grants from the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province(2022SDZG05)the National Natural Science Foundation of China(32270291,32061160467,31870171)to C.G.+7 种基金the Youth Innovation Promotion Association,Chinese Academy of Sciences(2023364)the Guangdong Basic and Applied Basic Research Foundation(2022A1515012319)the Guangzhou Basic and Applied Basic Research Foundation(2023A04J0094)to C.Y.the Sub-Project of Chinese Academy of Sciences Pilot Project(XDA24030502)the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology InnovationTeams(2020KJ148)to Y.W.the National Natural Science Foundation of China(32170362),the Guangdong Natural Science Funds for Distinguished Young Scholars(2022B1515020026)the Youth Innovation Promotion Association,Chinese Academy of Sciences(Y2021094)the Fund of South China Botanical Garden,Chinese Academy of Sciences(QNXM-02)to M.L.
文摘The bZIP transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)is a master regulator of seed germination and post-germinative growth in response to abscisic acid(ABA),but the detailed molecularmechanism by which it represses plant growth remains unclear.In this study,we used proximity labeling to map the neighboring proteome of ABI5 and identified FCS-LIKE ZINC FINGER PROTEIN 13(FLZ13)as a novel ABI5 interaction partner.Phenotypic analysis of flz13 mutants and FLZ13-overexpressing lines demonstrated that FLZ13 acts as a positive regulator of ABA signaling.Transcriptomic analysis revealed that both FLZ13 and ABI5 downregulate the expression of ABA-repressed and growth-related genes involved in chlorophyll biosynthesis,photosynthesis,and cell wall organization,thereby repressing seed germination and seedling establishment in response to ABA.Further genetic analysis showed that FLZ13 and ABI5 function together to regulate seed germination.Collectively,our findings reveal a previously uncharacterized transcriptional regulatorymechanismby which ABA mediates inhibition of seed germination and seedling establishment.
基金Work in Dr.Rodriguez's laboratory was funded by the Agencia Estatal de Investigacion of the Spanish Ministry of Science and Innovation with grant PID2020-113100RB-I00 funded by MCIN/AEI/10.13039/501100011033.
文摘Plants must adapt to unfavorable growth conditions such as drought,salinity,or extreme temperature and,in addition,need the flexibility to resume growth when conditions become favorable.Sensing of extracellular environmental stress and signal transduction rely on pathways that in many cases use phosphorylation of target proteins to amplify and transmit the extracellular signal.
基金supported by Agencia Estatal de Investigacion of the Spanish Ministry of Science and Innovation with grant numbers PID2020-119805RB-I00 and PID2023-1531080B-I00(A.A.)grant numbers PID2020-113100RB-I00 and PID2023-1473220OB-I00(P.L.R.)+7 种基金funded by MCIN/AEl/10.13039/501100011033 and the European Regional Development Fund(ERDF)("A way of making Europe")support from Agencia Estatal de Investigacion of the Spanish Ministry of Science and Innovation with grant number TED2021-129867B-C21(P.L.R.)grant number TED2021-132202B-I00(A.A.)funded by MCIU/AEI/10.13039/501100011033 and by the European Union NextGenerationEU/PRTR.J.C.Esupported by Agencia Estatal de Investigacion(CPP2021-009090,PID2022-136848NB-100),the Xunta de Galicia(ED431C 2022/21,ED431F 2020/05)Centro de investigacion do Sistema universitario de Galicia/accreditation 2023-2027,ED431G 2023/03the European Union(European Regional Development Fund--ERDF)supported by the Estonian Research Council(grant numberPRG 1620).
文摘Crop yield is at increasing risk due to water scarcity and climate change.Agrochemicals can activate hor-mone receptors to regulate transpiration and modulate transcription and address water deficits.Structure-guided optimization of multiple abscisic acid(ABA)receptor-agonist interactions is necessary to activate theentirePYRABACTINRESISTANCE1(PYR1)/PYR1-LIKE(PYL)/REGULATORYCOMPONENTSOFABA RECEPTORS(RCAR)receptor family.The new agonist iCB,produced through scaffold-merging led by X-ray structure,activates subfamilies Il and Ill at low-nM concentrations and subfamily I receptors at higher-nM concentrations.Structural analysis of opabactin and icB ternary complexes reveals selectivity-determining residues,making the PYL1/PYL4/PYL8 subfamilies sensitive to specific agonists and highlighting the differential sensitivity of receptor subfamilies to agonists across plant species.icB may activate mosteudicots'PYL8-like receptors,incontrastto opabactin,dueto limited steric constraints.This enables iCB to activate PYL8-like receptors with a bulkier Leu residue in the 3'tunnel,such as AtPYL8,SIPYL8,and VviPYL8.In contrast,opabactin activation is limited to receptors with Val at this position,for example,TaPYL8.Therefore,iCBextends its action to moreABAreceptors than CB,iSBo9,andopabactin,exhibits higher affinity than ABA for dimeric receptors,and can protect tomato plants against drought.In addition to regulating stomatal conductance and lowering water consumption,icB protects photosystem Il and improves photosynthesis following prolonged water deficit.Moreover,icB induces an ABA-like tran-scriptional response,upregulates the osmolyte synthesis,and can be hyperpotentiated when combined with the expression of a customized receptor.Our results provide structural insightsfor optimizing agonist designand aiding plants in managing water deficits.
