Objective:To search for the most active antimicrobial and antioxidant sub-fractions related to traditional use of Ziziphus oxyphylla(Z.oxyphylla)and Cedrela serrata(C.serrata)in Pakistan against infectious and liver d...Objective:To search for the most active antimicrobial and antioxidant sub-fractions related to traditional use of Ziziphus oxyphylla(Z.oxyphylla)and Cedrela serrata(C.serrata)in Pakistan against infectious and liver diseases.Methods:Factions of different polarity were tested in vitro for their antiprotozoal,antimalarial,antibacterial and antifungal activity against different pathogens.Cytotoxicity on MRC-5 cell lines(human lung fibroblasts)as well as,in vitro radical scavenging activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl radical assay.Results:The highest antiprotozoal activity was observed for the CHCl_(3)fractions of Z.oxyphylla roots and leaves,and C.serrata bark.The CHCl_(3)and Et OAc fractions of Z.oxyphylla roots,the CHCl_(3)fraction of Z.oxyphylla leaves,the Et OAc and the residual Me OH:H_(2)O fraction of C.serrata bark showed antibacterial activity against Staphylococcus aureus.The same residual Me OH:H_(2)O fraction of C.serrata bark was active against Candida albicans.The highest antioxidant activity was observed for the more hydrophilic Et OAc fractions of Z.oxyphylla leaves,C.serrata bark and leaves,and the residual Me OH:H_(2)O fraction of C.serrata bark.Conclusions:This study supports at least in part the traditional uses of these plants for antimicrobial purposes and against liver diseases.展开更多
基金the Higher Education Commission(HEC)of Pakistan for obtaining a fellowship with grant number(1-8/HEC/HRD/2011/2162)
文摘Objective:To search for the most active antimicrobial and antioxidant sub-fractions related to traditional use of Ziziphus oxyphylla(Z.oxyphylla)and Cedrela serrata(C.serrata)in Pakistan against infectious and liver diseases.Methods:Factions of different polarity were tested in vitro for their antiprotozoal,antimalarial,antibacterial and antifungal activity against different pathogens.Cytotoxicity on MRC-5 cell lines(human lung fibroblasts)as well as,in vitro radical scavenging activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl radical assay.Results:The highest antiprotozoal activity was observed for the CHCl_(3)fractions of Z.oxyphylla roots and leaves,and C.serrata bark.The CHCl_(3)and Et OAc fractions of Z.oxyphylla roots,the CHCl_(3)fraction of Z.oxyphylla leaves,the Et OAc and the residual Me OH:H_(2)O fraction of C.serrata bark showed antibacterial activity against Staphylococcus aureus.The same residual Me OH:H_(2)O fraction of C.serrata bark was active against Candida albicans.The highest antioxidant activity was observed for the more hydrophilic Et OAc fractions of Z.oxyphylla leaves,C.serrata bark and leaves,and the residual Me OH:H_(2)O fraction of C.serrata bark.Conclusions:This study supports at least in part the traditional uses of these plants for antimicrobial purposes and against liver diseases.
