The treatment of K.pneumoniae infections has become more difficult due to the emergence of multidrugresistant strains.This study aimed to evaluate antimicrobial resistance patterns and detect different types of beta-l...The treatment of K.pneumoniae infections has become more difficult due to the emergence of multidrugresistant strains.This study aimed to evaluate antimicrobial resistance patterns and detect different types of beta-lactamase genes among MDR and non-MDR Klebsiella pneumoniae in various clinical samples.A total of 150 Klebsiella pneumoniae were identified from different clinical samples by conventional microbiological procedures.Antibiotic sensitivity was detected by the Kirby-Bauer disc diffusion method.Extended-spectrum betalactamase,Metallo-β-lactamase,and carbapenemase were phenotypically detected by double-disk synergy test,combined disk assay,and modified Hodge test.PCR also detected differentβlactamase genes and virulence genes.The majority of Klebsiella pneumoniae were multi-drug resistant(82%).The bacteria were found resistant to mostβ-lactam antibiotics,aminoglycosides,ciprofloxacin,cotrimoxazole,carbapenem,piperacillin,and tazobactam.However,only about 0.7%colistin resistance was observed.A double-disk synergy test was performed among the isolates for phenotypic detection of ESBL producing Klebsiella pneumoniae.Only 16.6%of isolates were found to be ESBL producers.Amongβ-lactamase genes,NDM(23.34%),OXA-48(8%),and KPC(7.3%)were detected.Other prevalentβlactamase genes VIM and IMP were not detected.In summary,the prevalence of MDR Klebsiella pneumoniae is high in Bangladesh,which may complicate the treatment of hospitalized patients.展开更多
基金supported by the Department of Microbiology and Immunology,B中央人民政府,and the Research fund of Bangabandhu Sheikh Mujib Medical University(B中央人民政府).
文摘The treatment of K.pneumoniae infections has become more difficult due to the emergence of multidrugresistant strains.This study aimed to evaluate antimicrobial resistance patterns and detect different types of beta-lactamase genes among MDR and non-MDR Klebsiella pneumoniae in various clinical samples.A total of 150 Klebsiella pneumoniae were identified from different clinical samples by conventional microbiological procedures.Antibiotic sensitivity was detected by the Kirby-Bauer disc diffusion method.Extended-spectrum betalactamase,Metallo-β-lactamase,and carbapenemase were phenotypically detected by double-disk synergy test,combined disk assay,and modified Hodge test.PCR also detected differentβlactamase genes and virulence genes.The majority of Klebsiella pneumoniae were multi-drug resistant(82%).The bacteria were found resistant to mostβ-lactam antibiotics,aminoglycosides,ciprofloxacin,cotrimoxazole,carbapenem,piperacillin,and tazobactam.However,only about 0.7%colistin resistance was observed.A double-disk synergy test was performed among the isolates for phenotypic detection of ESBL producing Klebsiella pneumoniae.Only 16.6%of isolates were found to be ESBL producers.Amongβ-lactamase genes,NDM(23.34%),OXA-48(8%),and KPC(7.3%)were detected.Other prevalentβlactamase genes VIM and IMP were not detected.In summary,the prevalence of MDR Klebsiella pneumoniae is high in Bangladesh,which may complicate the treatment of hospitalized patients.
