The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related diseases.The influence of nanoparticle...The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related diseases.The influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of nanoparticles.Various physico-chemical parameters play a role in the interaction of proteins and nanoparticles in solution,thus influencing the disaggregation of preformed fibrils.We have examined the influence of two kinds of metallic nanoparticles on lysozyme amyloid fibrils using a multi-technique approach and focalized their impact on cytotoxicity on human neuroblastoma cells(SH-SY5Y).In particular,fluorescence,infrared and circular dichroism spectroscopies,optical and atomic force microscopy experiments have been carried out;the results are analyzed to rationalize the effects of these complexes on neural cell viability.It is remarkable,that the fibrils in the presence of AuNPs,unlike fibrils alone or with AgNPs,do not generate a significant cytotoxic effect even at high concentration and an amyloid degradation effect is visible.展开更多
Dense high-rise cities offer some advantages in terms of sustainability but have considerable downsides.Low-dense and medium-rise typologies have been shown to offer good social qualities;their potential energy and ca...Dense high-rise cities offer some advantages in terms of sustainability but have considerable downsides.Low-dense and medium-rise typologies have been shown to offer good social qualities;their potential energy and carbon advantages have received less attention.As the energy consumption,emissions of cities and heat island effects increase;we question whether dense,high-rise cities offer optimal sustainability.We discuss seven areas where medium density and lower rise typologies offer advantages in terms of energy and climate including:land use/density;microclimate/green space;energy supply;transports;operational energy/carbon;embodied energy/carbon;and resilience.The aim is to discuss the cumulative importance of these areas in the context of sustainable energy use and climate emissions.These areas are subject to ongoing research and are only discussed briefly,since the overarching synthesis perspective for urban planning is our focus.The picture that emerges when these points are seen together,suggests that medium density and lower rise options-like traditional European typologies-may offer,in addition to social qualities,very significant advantages in terms of energy,carbon and climate emissions.展开更多
基金This work was partially supported by Slovak grand agency VEGA 2/0145/17,APW-18-0284,Italian flagship NANOMAX,N-CHEM,Ministery o f Education,University and Research(PRIN grant 20173L7W8K).Microscopy was carried out at the SPM@ISMN facility.
文摘The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related diseases.The influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of nanoparticles.Various physico-chemical parameters play a role in the interaction of proteins and nanoparticles in solution,thus influencing the disaggregation of preformed fibrils.We have examined the influence of two kinds of metallic nanoparticles on lysozyme amyloid fibrils using a multi-technique approach and focalized their impact on cytotoxicity on human neuroblastoma cells(SH-SY5Y).In particular,fluorescence,infrared and circular dichroism spectroscopies,optical and atomic force microscopy experiments have been carried out;the results are analyzed to rationalize the effects of these complexes on neural cell viability.It is remarkable,that the fibrils in the presence of AuNPs,unlike fibrils alone or with AgNPs,do not generate a significant cytotoxic effect even at high concentration and an amyloid degradation effect is visible.
文摘Dense high-rise cities offer some advantages in terms of sustainability but have considerable downsides.Low-dense and medium-rise typologies have been shown to offer good social qualities;their potential energy and carbon advantages have received less attention.As the energy consumption,emissions of cities and heat island effects increase;we question whether dense,high-rise cities offer optimal sustainability.We discuss seven areas where medium density and lower rise typologies offer advantages in terms of energy and climate including:land use/density;microclimate/green space;energy supply;transports;operational energy/carbon;embodied energy/carbon;and resilience.The aim is to discuss the cumulative importance of these areas in the context of sustainable energy use and climate emissions.These areas are subject to ongoing research and are only discussed briefly,since the overarching synthesis perspective for urban planning is our focus.The picture that emerges when these points are seen together,suggests that medium density and lower rise options-like traditional European typologies-may offer,in addition to social qualities,very significant advantages in terms of energy,carbon and climate emissions.
