Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety.Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L.monocytogenes by int...Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety.Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L.monocytogenes by interacting with the cell membrane.This research further explored the antibacterial mechanism of brevilaterin B against L.monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis.Brevilaterin B induced growth inhibition rather than direct membrane lysis in L.monocytogenes at the minimum inhibitory concentration.Transcriptomic analysis showed 1779 difference expressed genes,including 895 up-regulated and 884 down-regulated genes.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L.monocytogenes,including peptidoglycan biosynthesis,membrane transport(ATP-binding cassette transports,ion transport),cellular metabolism(amino acid and lipid metabolism),ATP synthesis,and activation of the stress response(quorum sensing and bacterial chemotaxis).In conclusion,brevilaterin B affects gene expression related to biosynthesis,transport and stress response pathways on the membrane of L.monocytogenes.The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.展开更多
Cell senescence has attracted much attention in the long history of human beings,and telomere shortening(TS)is one of the main concerns in the study of cell senescence.To reveal the microscopic mechanism of TS process...Cell senescence has attracted much attention in the long history of human beings,and telomere shortening(TS)is one of the main concerns in the study of cell senescence.To reveal the microscopic mechanism of TS process,we model it based on molecular stochastic process from the perspective of nonequilibrium statistical physics.We associate the TS process with the continuous time random walk and derive the Fokker–Planck equation to describe the length distribution of the TS.We further modify the model describing the TS process,similar to the anomalous tempered diffusion,and derive the Feynman–Kac equation characterizing the functional distribution of the TS process.Finally,we study the statistics related to the critical telomere length l_(c),including the occupation time and first passage time.These two kinds of statistics help us understand the time scale of cell senescence.展开更多
基金financially supported by the National Natural Science Foundation of China(31771951,32072199,31801510)the Beijing Natural Science Foundation(KZ201810011016).
文摘Listeria monocytogenes is a worrisome food-borne pathogen threatening global food safety.Our previous study proved that lipopeptide brevilaterin B showed efficient antibacterial activity against L.monocytogenes by interacting with the cell membrane.This research further explored the antibacterial mechanism of brevilaterin B against L.monocytogenes at the sub-minimum inhibition concentration via transcriptomic analysis.Brevilaterin B induced growth inhibition rather than direct membrane lysis in L.monocytogenes at the minimum inhibitory concentration.Transcriptomic analysis showed 1779 difference expressed genes,including 895 up-regulated and 884 down-regulated genes.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that brevilaterin B influenced multiple pathways of L.monocytogenes,including peptidoglycan biosynthesis,membrane transport(ATP-binding cassette transports,ion transport),cellular metabolism(amino acid and lipid metabolism),ATP synthesis,and activation of the stress response(quorum sensing and bacterial chemotaxis).In conclusion,brevilaterin B affects gene expression related to biosynthesis,transport and stress response pathways on the membrane of L.monocytogenes.The present work provides the first transcriptomic assessment of the antibacterial mechanism of lipopeptide brevilaterin B at the gene level.
基金National Natural Science Foundation of China,Grant/Award Numbers:12225107,12071195Major Science and Technology Projects in Gansu Province-Leading Talents in Science and Technology,Grant/Award Number:23ZDKA0005+1 种基金Innovative Groups of Basic Research in Gansu Province,Grant/Award Number:22JR5RA391Lanzhou Talent Work Special Fund。
文摘Cell senescence has attracted much attention in the long history of human beings,and telomere shortening(TS)is one of the main concerns in the study of cell senescence.To reveal the microscopic mechanism of TS process,we model it based on molecular stochastic process from the perspective of nonequilibrium statistical physics.We associate the TS process with the continuous time random walk and derive the Fokker–Planck equation to describe the length distribution of the TS.We further modify the model describing the TS process,similar to the anomalous tempered diffusion,and derive the Feynman–Kac equation characterizing the functional distribution of the TS process.Finally,we study the statistics related to the critical telomere length l_(c),including the occupation time and first passage time.These two kinds of statistics help us understand the time scale of cell senescence.
