Introduction:Southern Xinjiang Uygur Autonomous Region,China,experiences a substantial tuberculosis burden,yet comprehensive genotypic characterization of Mycobacterium tuberculosis strains in this region remains limi...Introduction:Southern Xinjiang Uygur Autonomous Region,China,experiences a substantial tuberculosis burden,yet comprehensive genotypic characterization of Mycobacterium tuberculosis strains in this region remains limited.Methods:This study collected Mycobacterium tuberculosis strains and corresponding epidemiological data from patients between 2021 and 2023.Bacterial isolates underwent whole-genome sequencing using the Illumina next-generation sequencing platform.We constructed phylogenetic trees using iqtree and generated minimum spanning trees based on GraphSNP analysis,applying a clustering threshold of 12 single nucleotide polymorphisms(SNPs)to identify transmission clusters.Results:Lineage 2 emerged as the predominant strain type,accounting for the majority of isolates,followed by Lineages 4 and 3.Notably,Lineage 2 demonstrated significantly elevated clustering rates compared to other lineages.Genetic diversity analysis revealed that Lineage 2 strains exhibited the most limited intra-lineage variation,whereas Lineage 3 displayed the greatest genetic heterogeneity among strains.Conclusion:Our investigation demonstrates substantial genetic polymorphism among Mycobacterium tuberculosis strains circulating in southern Xinjiang.These findings highlight the critical need for enhanced transmission control strategies,with particular emphasis on intensive surveillance and prevention measures targeting Lineage 2 strains.展开更多
基金Supported by the“Tianshan Talents”Medical and Health High-level Talent Training Program(TSYC202301B166)the Natural Science Foundation of Xinjiang Uygur Autonomous Region Project(2023D01C57),and grant WJW2024-087.
文摘Introduction:Southern Xinjiang Uygur Autonomous Region,China,experiences a substantial tuberculosis burden,yet comprehensive genotypic characterization of Mycobacterium tuberculosis strains in this region remains limited.Methods:This study collected Mycobacterium tuberculosis strains and corresponding epidemiological data from patients between 2021 and 2023.Bacterial isolates underwent whole-genome sequencing using the Illumina next-generation sequencing platform.We constructed phylogenetic trees using iqtree and generated minimum spanning trees based on GraphSNP analysis,applying a clustering threshold of 12 single nucleotide polymorphisms(SNPs)to identify transmission clusters.Results:Lineage 2 emerged as the predominant strain type,accounting for the majority of isolates,followed by Lineages 4 and 3.Notably,Lineage 2 demonstrated significantly elevated clustering rates compared to other lineages.Genetic diversity analysis revealed that Lineage 2 strains exhibited the most limited intra-lineage variation,whereas Lineage 3 displayed the greatest genetic heterogeneity among strains.Conclusion:Our investigation demonstrates substantial genetic polymorphism among Mycobacterium tuberculosis strains circulating in southern Xinjiang.These findings highlight the critical need for enhanced transmission control strategies,with particular emphasis on intensive surveillance and prevention measures targeting Lineage 2 strains.
