With the perspective of food safety and safer consumption,this study examined the insecticidal potentiality of ten different chemical compounds against the pulse beetle pest,Callosobruchus maculatus.Insecticidal prope...With the perspective of food safety and safer consumption,this study examined the insecticidal potentiality of ten different chemical compounds against the pulse beetle pest,Callosobruchus maculatus.Insecticidal property of chemical compounds were analyzed by fumigant toxicity assay and probit analysis was performed for predicting the lethal concentrations.After 72 h of fumigation,persistence of fumigant residues was determined from the treated C.maculatus adults by gas chromatography-mass spectroscopy(GC-MS)analysis.Further,cytotoxicity of the highly effective fumigant was examined using human liver cancer cells(Huh-7)by MTT assay.Fumigant toxicity assay exhibited 1.60μL/L of air LC50 value and 694.38 FTI(@72 h of treatment)for diallyl disulfide as highly potent fumigant compared to other Volatile Organic Compounds(VOCs)against C.maculatus.In GC-MS analysis,46.53 ppm/adult of diallyl disulfide persistence was determined in the insect.Next to diallyl disulfide,the eucalyptol was identified as effective fumigant with 4.64μL/L of air LC50 value for 72 h of fumigation exposure.Treatment with increasing concentration of diallyl disulfide(1-300μM)affected the viability of Huh-7 cells in a dose dependent manner.Overall,the results suggested that diallyl disulfide is a potent fumigant to C.maculatus and the safety of diallyl disulfide residues for consumption warrants further investigation.展开更多
基金funded by the Council of Scientific and Industrial Research(CSIR)Ministry of Science and TechnologyGovernment of India,under the scheme CSIR-Fundamental and Innovative Research in Science of Tomorrow(CSIR-FIRST)(Grant Ref.No.MLP300).
文摘With the perspective of food safety and safer consumption,this study examined the insecticidal potentiality of ten different chemical compounds against the pulse beetle pest,Callosobruchus maculatus.Insecticidal property of chemical compounds were analyzed by fumigant toxicity assay and probit analysis was performed for predicting the lethal concentrations.After 72 h of fumigation,persistence of fumigant residues was determined from the treated C.maculatus adults by gas chromatography-mass spectroscopy(GC-MS)analysis.Further,cytotoxicity of the highly effective fumigant was examined using human liver cancer cells(Huh-7)by MTT assay.Fumigant toxicity assay exhibited 1.60μL/L of air LC50 value and 694.38 FTI(@72 h of treatment)for diallyl disulfide as highly potent fumigant compared to other Volatile Organic Compounds(VOCs)against C.maculatus.In GC-MS analysis,46.53 ppm/adult of diallyl disulfide persistence was determined in the insect.Next to diallyl disulfide,the eucalyptol was identified as effective fumigant with 4.64μL/L of air LC50 value for 72 h of fumigation exposure.Treatment with increasing concentration of diallyl disulfide(1-300μM)affected the viability of Huh-7 cells in a dose dependent manner.Overall,the results suggested that diallyl disulfide is a potent fumigant to C.maculatus and the safety of diallyl disulfide residues for consumption warrants further investigation.
