To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experi...To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the medium was replaced with induction medium, which contained GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments. The surface markers of the differentiated neurons, such as NSE, nestin, MAP-2a, b and TH were detected by immunocytochemistry and Western blot after MSCs were cultured in induction medium for 7 days and 15 days. Results MSCs differentiated into neural progenitors and expressed nestin after MSCs were incubated with medium containing IBMX for 2 d. After the medium was replaced with induction medium containing many inducing agents, MSCs differentiated into neuron-like cells and dopaminergic neuron-like cells and expressed NSE, MAP-2a, b and TH. The percentage of NSE-positive cells, MAP-2a, b-positive cells and TH-positive cells was 30.032±2.489%, 41.580±5.101% and 34.958±5.534%, respectively after MSCs were induced in medium containing GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments for 15 days. Conclusion MSCs can differentiate into dopaminergic neuron-like cells and are a new cell source for the treatment of neurodegeneration diseases and have a great potential for wide application展开更多
Objective: to evaluate the directional therapeutic effect of severe ultrasound, PCUM, adjusted pulmonary window, pulmonary ultrasound examination and high-frequency chest wall oscillation sputum discharge system in th...Objective: to evaluate the directional therapeutic effect of severe ultrasound, PCUM, adjusted pulmonary window, pulmonary ultrasound examination and high-frequency chest wall oscillation sputum discharge system in the diagnosis and treatment process of children with severe pneumonia. Method: select from December 2020 to December 2021,256 cases with obvious imaging changes in severe pneumonia, using severe ultrasound PCUM adjust lung window lung ultrasound, combined with its imaging performance, directional implementation of high frequency chest wall oscillation sputum treatment and drainage, treatment before and after the comparison of the oxygenation index, mechanical ventilation mechanical index, imaging changes, evaluate the treatment effect. Results: in 256 children with severe pneumonia, high-frequency chest wall oscillation sputum discharge system significantly improved symptoms, shortened mechanical ventilation time and reduced man-machine confrontation. Conclusion: PCUM adjusted lung window lung ultrasound examination combined with high-frequency chest wall oscillation sputum drainage system treatment can effectively improve the oxygenation, and can also can effectively prevent the occurrence of ventilator-related pneumonia.展开更多
With the development and progress of the times, in the process of social development, Chinas technical and skilled personnel in various fields gradually appear the double dilemma of insufficient supply and difficult e...With the development and progress of the times, in the process of social development, Chinas technical and skilled personnel in various fields gradually appear the double dilemma of insufficient supply and difficult employment for graduates, which has had a huge impact on the stable development of society and the progress of the country. Higher vocational colleges are an important field for the development and training of technical talents in China. Improving the quality training of technical talents in higher vocational colleges can effectively lay the foundation for the development of various fields in the country. Therefore, in the process of operation and management, higher vocational colleges need to position the development goal on the cultivation of high-quality technical personnel, effectively integrate quality education with technical skills training, and educate high-quality technical personnel that meet the development of the times and social progress. In the process of talent management and cultivation, higher vocational colleges need to grasp the market demand, keep up with the trend of social development, learn from domestic and foreign educational experience, and cultivate talents that meet the market demand for national development and social progress.展开更多
基金This work was supported by a grant from the National Natural Science Foundation of China (No.39970741) a grant from the the Scienceand Technology Foundation of Jilin Health Administration (No. 200131) and a grant from the Youth Teacher Foundation o
文摘To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the medium was replaced with induction medium, which contained GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments. The surface markers of the differentiated neurons, such as NSE, nestin, MAP-2a, b and TH were detected by immunocytochemistry and Western blot after MSCs were cultured in induction medium for 7 days and 15 days. Results MSCs differentiated into neural progenitors and expressed nestin after MSCs were incubated with medium containing IBMX for 2 d. After the medium was replaced with induction medium containing many inducing agents, MSCs differentiated into neuron-like cells and dopaminergic neuron-like cells and expressed NSE, MAP-2a, b and TH. The percentage of NSE-positive cells, MAP-2a, b-positive cells and TH-positive cells was 30.032±2.489%, 41.580±5.101% and 34.958±5.534%, respectively after MSCs were induced in medium containing GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments for 15 days. Conclusion MSCs can differentiate into dopaminergic neuron-like cells and are a new cell source for the treatment of neurodegeneration diseases and have a great potential for wide application
文摘Objective: to evaluate the directional therapeutic effect of severe ultrasound, PCUM, adjusted pulmonary window, pulmonary ultrasound examination and high-frequency chest wall oscillation sputum discharge system in the diagnosis and treatment process of children with severe pneumonia. Method: select from December 2020 to December 2021,256 cases with obvious imaging changes in severe pneumonia, using severe ultrasound PCUM adjust lung window lung ultrasound, combined with its imaging performance, directional implementation of high frequency chest wall oscillation sputum treatment and drainage, treatment before and after the comparison of the oxygenation index, mechanical ventilation mechanical index, imaging changes, evaluate the treatment effect. Results: in 256 children with severe pneumonia, high-frequency chest wall oscillation sputum discharge system significantly improved symptoms, shortened mechanical ventilation time and reduced man-machine confrontation. Conclusion: PCUM adjusted lung window lung ultrasound examination combined with high-frequency chest wall oscillation sputum drainage system treatment can effectively improve the oxygenation, and can also can effectively prevent the occurrence of ventilator-related pneumonia.
文摘With the development and progress of the times, in the process of social development, Chinas technical and skilled personnel in various fields gradually appear the double dilemma of insufficient supply and difficult employment for graduates, which has had a huge impact on the stable development of society and the progress of the country. Higher vocational colleges are an important field for the development and training of technical talents in China. Improving the quality training of technical talents in higher vocational colleges can effectively lay the foundation for the development of various fields in the country. Therefore, in the process of operation and management, higher vocational colleges need to position the development goal on the cultivation of high-quality technical personnel, effectively integrate quality education with technical skills training, and educate high-quality technical personnel that meet the development of the times and social progress. In the process of talent management and cultivation, higher vocational colleges need to grasp the market demand, keep up with the trend of social development, learn from domestic and foreign educational experience, and cultivate talents that meet the market demand for national development and social progress.
