为探究南五味子属植物叶绿体基因组密码子的使用偏好性及影响因素,利用Codon W 1.4.2、CUSP及EMBOSS等程序,进行中性绘图、ENC-plot及PR2-plot绘图分析。结果表明,南五味子属4个物种的叶绿体基因组密码子总体偏性较弱,只有rps14的ENC值...为探究南五味子属植物叶绿体基因组密码子的使用偏好性及影响因素,利用Codon W 1.4.2、CUSP及EMBOSS等程序,进行中性绘图、ENC-plot及PR2-plot绘图分析。结果表明,南五味子属4个物种的叶绿体基因组密码子总体偏性较弱,只有rps14的ENC值小于40;物种间的密码子参数高度保守,GC_(2)对ENC影响较大;有31个密码子RSCU大于1,均以A/U结尾;自然选择在4个物种叶绿体基因组同义密码子使用偏性中发挥主导作用;南五味子属4个物种中分别确定了18、17、16和18个最优密码子,共有的最优密码子有14个;在ΔR_(SCU)>0.5的高频密码子中,仅有编码半胱氨酸(Cys)的UGU为共有最优密码子。表明南五味子属4个物种间密码子偏好性较保守,自然选择是影响密码子偏好性的主要因素。研究结果为南五味子属物种鉴定、保护和遗传改良提供了基础。展开更多
Variants in chicken growth hormone receptor （GHR） gene lead to sex-linked dwarf （SLD） chickens, but effects of different variants are distinct. In this study, 11 SLD chicken breeds or strains including 3 Chinese n...Variants in chicken growth hormone receptor （GHR） gene lead to sex-linked dwarf （SLD） chickens, but effects of different variants are distinct. In this study, 11 SLD chicken breeds or strains including 3 Chinese native breeds and 8 breeding strains were studied in order to investigate the effects of different sex-linked dwarf variations on growth performance. The results showed that there were three reasons which could lead to dwarfism in the 11 breeds or strains. Firstly, an about 1.7 kb deletion of growth hormone receptor （GHR） gene leads to dwarfism in Jiangxi dwarf chicken, strains GF24, GF26, N308, N309, and N310. Secondly, a T354C mutation in exon 5 of the GHR gene leads to dwarfism in strains N301 and N305. Thirdly, an unknown variant leads to dwarfism in Guizhou Yellow Dwarf chicken and Yixing Bantam chicken. In addition, all individuals of N303 had the 1.7 kb deletion of the GHR gene, and additionally, some of them also carried the T354C mutation. As far as the performance of individuals were compared among T354C homozygote, deletion homozygote, and heterozygote carrying both T354C and deletion, it was found that the T354C＇s impacts on body weight of Chinese chickens were maximum, the body weight of chickens with homozygote T354C was 92.12% of those with heterozygote, and the difference of the body weight between deletion homozygote and heterozygote was not significant. There was no significant difference of shank length among three genotypes.展开更多
Objective:To evaluate the efficacy and safety of Wuda Granule(WDG)on recovery of gastrointestinal function after laparoscopic bowel resection in the setting of enhanced recovery after surgery(ERAS)-based perioperative...Objective:To evaluate the efficacy and safety of Wuda Granule(WDG)on recovery of gastrointestinal function after laparoscopic bowel resection in the setting of enhanced recovery after surgery(ERAS)-based perioperative care.Methods:A total of 108 patients aged 18 years or older undergoing laparoscopic bowel resection with a surgical duration of 2 to 4.5 h were randomly assigned(1:1)to receive either WDG or placebo(10 g/bag)twice a day from postoperative days 1-3,combining with ERAS-based perioperative care.The primary outcome was time to first defecation.Secondary outcomes were time to first flatus,time to first tolerance of liquid or semi-liquid food,gastrointestinal-related symptoms and length of stay.Subgroup analysis of the primary outcome according to sex,age,tumor site,surgical time,histories of underlying disease or history of abdominal surgery was undertaken.Adverse eventse were observed and recorded.Results:A total of 107patients[53 in the WDG group and 54 in the placebo group;61.7±12.1 years;50 males(46.7%)]were included in the intention-to-treat analysis.The patients in the WDG group had a significantly shorter time to first defecation and flatus[between-group difference-11.01 h(95%CI-20.75 to-1.28 h),P=0.012 for defecation;-5.41 h(-11.10 to 0.27 h),P=0.040 for flatus]than the placebo group.Moreover,the extent of improvement in postoperative gastrointestinal-related symptoms in the WDG group was significantly better than that in the placebo group(P<0.05).Subgroup analyses revealed that the benefits of WDG were significantly superior in patients who were male,or under 60 years old,or surgical time less than 3 h,or having no history of basic disease or no history of abdominal surgery.There were no serious adverse events.Conclusion:The addition of WDG to an ERAS postoperative care may be a viable strategy to enhance gastrointestinal function recovery after laparoscopic bowel resection surgery.(Registry No.ChiCTR2100046242)展开更多
文摘为探究南五味子属植物叶绿体基因组密码子的使用偏好性及影响因素,利用Codon W 1.4.2、CUSP及EMBOSS等程序,进行中性绘图、ENC-plot及PR2-plot绘图分析。结果表明,南五味子属4个物种的叶绿体基因组密码子总体偏性较弱,只有rps14的ENC值小于40;物种间的密码子参数高度保守,GC_(2)对ENC影响较大;有31个密码子RSCU大于1,均以A/U结尾;自然选择在4个物种叶绿体基因组同义密码子使用偏性中发挥主导作用;南五味子属4个物种中分别确定了18、17、16和18个最优密码子,共有的最优密码子有14个;在ΔR_(SCU)>0.5的高频密码子中,仅有编码半胱氨酸(Cys)的UGU为共有最优密码子。表明南五味子属4个物种间密码子偏好性较保守,自然选择是影响密码子偏好性的主要因素。研究结果为南五味子属物种鉴定、保护和遗传改良提供了基础。
基金projects under the Major State Basic Research Development Program China(2006CB102107)the earmarked fund for Modern Agro-Industry Technology Research System (nycytx-42-G1-04)the National High Technology Research and Development Program of China (863 Program,2010AA10A102)
文摘Variants in chicken growth hormone receptor （GHR） gene lead to sex-linked dwarf （SLD） chickens, but effects of different variants are distinct. In this study, 11 SLD chicken breeds or strains including 3 Chinese native breeds and 8 breeding strains were studied in order to investigate the effects of different sex-linked dwarf variations on growth performance. The results showed that there were three reasons which could lead to dwarfism in the 11 breeds or strains. Firstly, an about 1.7 kb deletion of growth hormone receptor （GHR） gene leads to dwarfism in Jiangxi dwarf chicken, strains GF24, GF26, N308, N309, and N310. Secondly, a T354C mutation in exon 5 of the GHR gene leads to dwarfism in strains N301 and N305. Thirdly, an unknown variant leads to dwarfism in Guizhou Yellow Dwarf chicken and Yixing Bantam chicken. In addition, all individuals of N303 had the 1.7 kb deletion of the GHR gene, and additionally, some of them also carried the T354C mutation. As far as the performance of individuals were compared among T354C homozygote, deletion homozygote, and heterozygote carrying both T354C and deletion, it was found that the T354C＇s impacts on body weight of Chinese chickens were maximum, the body weight of chickens with homozygote T354C was 92.12% of those with heterozygote, and the difference of the body weight between deletion homozygote and heterozygote was not significant. There was no significant difference of shank length among three genotypes.
基金Supported by the Special Subsidy for Clinical Research of Guangdong Provincial Hospital of Chinese Medicine(No.YN10101902)the Double First-Class and High-Level University Discipline Collaborative Innovation Team Project of Guangzhou University of Chinese Medicine(No.2021xk48)。
文摘Objective:To evaluate the efficacy and safety of Wuda Granule(WDG)on recovery of gastrointestinal function after laparoscopic bowel resection in the setting of enhanced recovery after surgery(ERAS)-based perioperative care.Methods:A total of 108 patients aged 18 years or older undergoing laparoscopic bowel resection with a surgical duration of 2 to 4.5 h were randomly assigned(1:1)to receive either WDG or placebo(10 g/bag)twice a day from postoperative days 1-3,combining with ERAS-based perioperative care.The primary outcome was time to first defecation.Secondary outcomes were time to first flatus,time to first tolerance of liquid or semi-liquid food,gastrointestinal-related symptoms and length of stay.Subgroup analysis of the primary outcome according to sex,age,tumor site,surgical time,histories of underlying disease or history of abdominal surgery was undertaken.Adverse eventse were observed and recorded.Results:A total of 107patients[53 in the WDG group and 54 in the placebo group;61.7±12.1 years;50 males(46.7%)]were included in the intention-to-treat analysis.The patients in the WDG group had a significantly shorter time to first defecation and flatus[between-group difference-11.01 h(95%CI-20.75 to-1.28 h),P=0.012 for defecation;-5.41 h(-11.10 to 0.27 h),P=0.040 for flatus]than the placebo group.Moreover,the extent of improvement in postoperative gastrointestinal-related symptoms in the WDG group was significantly better than that in the placebo group(P<0.05).Subgroup analyses revealed that the benefits of WDG were significantly superior in patients who were male,or under 60 years old,or surgical time less than 3 h,or having no history of basic disease or no history of abdominal surgery.There were no serious adverse events.Conclusion:The addition of WDG to an ERAS postoperative care may be a viable strategy to enhance gastrointestinal function recovery after laparoscopic bowel resection surgery.(Registry No.ChiCTR2100046242)
