旨在挖掘绵羊中与优异性状相关基因,对遗传改良与育种实践具有重要价值。本研究以93只策勒黑羊、33只皮山红羊和13只瓦格吉尔羊为对象,进行颈静脉采血、DNA提取、基因分型。用PLINK软件对基因型数据进行质量控制(质控标准为:剔除个体检...旨在挖掘绵羊中与优异性状相关基因,对遗传改良与育种实践具有重要价值。本研究以93只策勒黑羊、33只皮山红羊和13只瓦格吉尔羊为对象,进行颈静脉采血、DNA提取、基因分型。用PLINK软件对基因型数据进行质量控制(质控标准为:剔除个体检出率小于90%、SNP检出率小于95%、最小等位基因频率小于5%、哈代温伯格平衡P<1×10-6的SNPs)和主成分分析(PCA),构建进化树、群体祖先成分分析和连锁不平衡分析(LD),同时基于全基因组长纯合片段(runs of homozygosity,ROH)分析和群体遗传分化指数(F_(ST))分析,选择前10%的ROH片段作为高频区域和F_(ST)值的前5%位点作为受选择区域,参考绵羊基因组Oar_v4.0注释基因并进行GO和KEGG分析。F_(ST)和FROH结果表明,3个群体之间遗传分化水平较低,且在K=4呈现清晰的遗传背景分化。同时识别出ACVR1、ACVR1C、UPP2、CRY1和NR4A2等是南疆地方绵羊品种在干旱沙漠环境中形成遗传适应性的候选基因。本研究通过群体遗传结构多样性和选择信号分析,揭示了南疆地方绵羊品种的遗传变异特征,从多维度的遗传变异视角寻找到相关优异基因,为绵羊种质资源保护、新品种培育及资源多样性提升提供了重要参考依据。展开更多
In recent years, a few researches focus on the similarity measure of semantic trajectories in road networks, since semantic trajectories in road networks have smaller volumes, higher qualities and can better reflect u...In recent years, a few researches focus on the similarity measure of semantic trajectories in road networks, since semantic trajectories in road networks have smaller volumes, higher qualities and can better reflect user behaviors. However, these works do not further discuss how to efficiently search similar trajectories. Thus, to implement an efficient similarity search, we design an index called SIET based on the structures of road networks. Then, we propose a novel algorithm called SSN-BF to search similar trajectories efficiently by using best-first strategy. At last, we take the experimental evaluations on real dataset and prove the efficiency of our algorithm.展开更多
文摘旨在挖掘绵羊中与优异性状相关基因,对遗传改良与育种实践具有重要价值。本研究以93只策勒黑羊、33只皮山红羊和13只瓦格吉尔羊为对象,进行颈静脉采血、DNA提取、基因分型。用PLINK软件对基因型数据进行质量控制(质控标准为:剔除个体检出率小于90%、SNP检出率小于95%、最小等位基因频率小于5%、哈代温伯格平衡P<1×10-6的SNPs)和主成分分析(PCA),构建进化树、群体祖先成分分析和连锁不平衡分析(LD),同时基于全基因组长纯合片段(runs of homozygosity,ROH)分析和群体遗传分化指数(F_(ST))分析,选择前10%的ROH片段作为高频区域和F_(ST)值的前5%位点作为受选择区域,参考绵羊基因组Oar_v4.0注释基因并进行GO和KEGG分析。F_(ST)和FROH结果表明,3个群体之间遗传分化水平较低,且在K=4呈现清晰的遗传背景分化。同时识别出ACVR1、ACVR1C、UPP2、CRY1和NR4A2等是南疆地方绵羊品种在干旱沙漠环境中形成遗传适应性的候选基因。本研究通过群体遗传结构多样性和选择信号分析,揭示了南疆地方绵羊品种的遗传变异特征,从多维度的遗传变异视角寻找到相关优异基因,为绵羊种质资源保护、新品种培育及资源多样性提升提供了重要参考依据。
基金Supported by the National Key Research and Development Program of the Ministry of Science and Technology of China(2016YFB1000700)
文摘In recent years, a few researches focus on the similarity measure of semantic trajectories in road networks, since semantic trajectories in road networks have smaller volumes, higher qualities and can better reflect user behaviors. However, these works do not further discuss how to efficiently search similar trajectories. Thus, to implement an efficient similarity search, we design an index called SIET based on the structures of road networks. Then, we propose a novel algorithm called SSN-BF to search similar trajectories efficiently by using best-first strategy. At last, we take the experimental evaluations on real dataset and prove the efficiency of our algorithm.
