[目的]分析针灸治疗中风后构音障碍的临床文献,以探讨针灸治疗该病的取穴规律及刺灸方法。[方法]检索中国知网、万方数据库、维普网、中国生物医学数据库、Pubmed、Web of Science自建库至2024年7月收录的针灸治疗中风后构音障碍的临床...[目的]分析针灸治疗中风后构音障碍的临床文献,以探讨针灸治疗该病的取穴规律及刺灸方法。[方法]检索中国知网、万方数据库、维普网、中国生物医学数据库、Pubmed、Web of Science自建库至2024年7月收录的针灸治疗中风后构音障碍的临床随机对照试验的文献,应用Excel2016建立针灸处方数据库,运用SPSS 25.0进行频次分析、聚类分析,Cytoscape进行关联规则分析。[结果]本研究共纳入相关临床研究文献93篇,涉及治疗该病的腧穴总数有89个,取穴总频次为652次。分析得出使用频次前五的腧穴依次为风池(56次)、金津(56次)、玉液(56次)、廉泉(42次)、百会(32次)。督脉为常用经脉,腧穴使用频次和个数均高于其他经脉。在特定穴中,络穴使用频次最高(35次),其中通里使用次数最多(17次)。关联规则显示金津-玉液的支持度最高,其次为金津-玉液-风池。聚类分析得出4个有效聚类群。刺灸方法以毫针针刺为主,其次为三棱针点刺放血。[结论]针刺治疗中风后构音障碍以近部取穴为主,针刺舌咽及头颈附近穴位配合督脉穴位能够有效改善构音障碍。展开更多
OBJECTIVE:To examine the effect of Xuanfei Tongqiao acupuncture on nasal inflammation in rats with allergic rhinitis(AR)and its underlying mechanisms,particularly focusing on the role of acupuncture in regulating the ...OBJECTIVE:To examine the effect of Xuanfei Tongqiao acupuncture on nasal inflammation in rats with allergic rhinitis(AR)and its underlying mechanisms,particularly focusing on the role of acupuncture in regulating the methylation of long non-coding RNA growth arrestspecific transcript 5(lnc RNA GAS5).METHODS:Interventions involved acupuncture or short hairpin RNA-GAS5(sh-GAS5)lentivirus in an ovalbumininduced AR rat model.Nasal mucosal tissues were collected post-intervention for pathological analysis.Parameters assessed included the proportion of type 2 innate lymphoid cells(ILC2s),the expression of GATA binding protein 3(GATA3),methyltransferase-like 3(METTL3),interleukin(IL)-33 proteins,and the expression of METTL3 and lnc RNA-GAS5.RESULTS:Xuanfei Tongqiao acupuncture and/or lentivirus sh-GAS5 significantly alleviated rhinitis symptoms and reduced nasal inflammation in AR rats.This was evidenced by a reduction in inflammatory cells,eosinophils,and cuprocytes in nasal mucosal tissues,along with a decreased proportion of ILC2s.Furthermore,protein levels of GATA3,METTL3,and IL-33,as well as the expression of METTL3 and lnc RNA-GAS5,were significantly reduced.These findings suggest that lnc RNA-GAS5 and its N6-methyladenosine methylation are key regulators of AR inflammation,with acupuncture exerting a modulatory effect on this pathway.CONCLUSIONS:Xuanfei Tongqiao acupuncture therapy inhibited the METTL3-GAS5-GATA3-IL-33-ILC2 pathway,reducing the inflammatory response and alleviating nasal symptoms in AR rats.These results suggest that Xuanfei Tongqiao acupuncture could serve as a potential alternative therapy for preventing and managing AR associated with inflammation.展开更多
文摘[目的]分析针灸治疗中风后构音障碍的临床文献,以探讨针灸治疗该病的取穴规律及刺灸方法。[方法]检索中国知网、万方数据库、维普网、中国生物医学数据库、Pubmed、Web of Science自建库至2024年7月收录的针灸治疗中风后构音障碍的临床随机对照试验的文献,应用Excel2016建立针灸处方数据库,运用SPSS 25.0进行频次分析、聚类分析,Cytoscape进行关联规则分析。[结果]本研究共纳入相关临床研究文献93篇,涉及治疗该病的腧穴总数有89个,取穴总频次为652次。分析得出使用频次前五的腧穴依次为风池(56次)、金津(56次)、玉液(56次)、廉泉(42次)、百会(32次)。督脉为常用经脉,腧穴使用频次和个数均高于其他经脉。在特定穴中,络穴使用频次最高(35次),其中通里使用次数最多(17次)。关联规则显示金津-玉液的支持度最高,其次为金津-玉液-风池。聚类分析得出4个有效聚类群。刺灸方法以毫针针刺为主,其次为三棱针点刺放血。[结论]针刺治疗中风后构音障碍以近部取穴为主,针刺舌咽及头颈附近穴位配合督脉穴位能够有效改善构音障碍。
基金Open Project of National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion:Multi-Center Clinical Study on Xuanfei Tongqiao Acupuncture combined with Sphenopalatine Ganglion Stimulation in the Treatment of Allergic Rhinitis(No.NCRCOP2023008)Foundation of First Teaching Hospital of Tianjin University of Traditional Chinese Medicine:Clinical Observation on Preventive Acupuncture for Seasonal Allergic Rhinitis based on the Theory of"Strong Spleen Prevents Pathogenic Invasion"(No.2020043)the Medical Research Project of Yancheng City Health Commission:Mechanism of Xuanfei Tongqiao Acupuncture in Treating Allergic Rhinitis via m6A Methylation Modification of lncRNA GAS5(No.YK2023017)。
文摘OBJECTIVE:To examine the effect of Xuanfei Tongqiao acupuncture on nasal inflammation in rats with allergic rhinitis(AR)and its underlying mechanisms,particularly focusing on the role of acupuncture in regulating the methylation of long non-coding RNA growth arrestspecific transcript 5(lnc RNA GAS5).METHODS:Interventions involved acupuncture or short hairpin RNA-GAS5(sh-GAS5)lentivirus in an ovalbumininduced AR rat model.Nasal mucosal tissues were collected post-intervention for pathological analysis.Parameters assessed included the proportion of type 2 innate lymphoid cells(ILC2s),the expression of GATA binding protein 3(GATA3),methyltransferase-like 3(METTL3),interleukin(IL)-33 proteins,and the expression of METTL3 and lnc RNA-GAS5.RESULTS:Xuanfei Tongqiao acupuncture and/or lentivirus sh-GAS5 significantly alleviated rhinitis symptoms and reduced nasal inflammation in AR rats.This was evidenced by a reduction in inflammatory cells,eosinophils,and cuprocytes in nasal mucosal tissues,along with a decreased proportion of ILC2s.Furthermore,protein levels of GATA3,METTL3,and IL-33,as well as the expression of METTL3 and lnc RNA-GAS5,were significantly reduced.These findings suggest that lnc RNA-GAS5 and its N6-methyladenosine methylation are key regulators of AR inflammation,with acupuncture exerting a modulatory effect on this pathway.CONCLUSIONS:Xuanfei Tongqiao acupuncture therapy inhibited the METTL3-GAS5-GATA3-IL-33-ILC2 pathway,reducing the inflammatory response and alleviating nasal symptoms in AR rats.These results suggest that Xuanfei Tongqiao acupuncture could serve as a potential alternative therapy for preventing and managing AR associated with inflammation.
