本研究基于Web of Science数据库核心合集,借助CiteSpace梳理分析了近十年来(2011—2021年)国际计算机辅助语言学习研究的热点和发展趋势。分析结果显示,在人工智能背景下,虚拟现实、远程协作、语音识别、人机互动、自动测评等技术的应...本研究基于Web of Science数据库核心合集,借助CiteSpace梳理分析了近十年来(2011—2021年)国际计算机辅助语言学习研究的热点和发展趋势。分析结果显示,在人工智能背景下,虚拟现实、远程协作、语音识别、人机互动、自动测评等技术的应用促使外语学习方式发生巨大变革。非正式数字化学习、智能语言学习系统、混合式学习、数据驱动学习等话题是该领域研究的热点。研究进而总结了新技术的广泛应用推动该领域呈现的发展趋势,并提出对今后相关研究的启示。展开更多
Class Ⅲ β-tubulin （Tubb3） is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel...Class Ⅲ β-tubulin （Tubb3） is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel bacterial artificial chromosome （BAC） transgenic mouse line that expresses Class Ⅲ β-tubulin fused to mCherry, an improved monomeric red fluorescent protein, for the visualization of microtubules during neuronal development. A BAC containing Tubb3 gene was modified by insertion of mCherry complementary DNA downstream of Tubb3 coding sequence via homologous recombination, mCherry fusion protein was expressed in the nervous system and testis of the transgenic animal, and the fluorescent signal was observed in the neurons that located in the olfactory bulb, cerebral cortex, hippocampal formation, cerebellum, as well as the retina. Besides, Tubb3-mCherry fusion protein mainly distributed in neurites and colocalized with endogenous Class Ⅲ β-tubulin The fusion protein labels Purkinje cell dendrites during cerebellar circuit formation. Therefore, this transgenic line might be a novel tool for scientific community to study neuronal development both in vitro and in vivo.展开更多
文摘本研究基于Web of Science数据库核心合集,借助CiteSpace梳理分析了近十年来(2011—2021年)国际计算机辅助语言学习研究的热点和发展趋势。分析结果显示,在人工智能背景下,虚拟现实、远程协作、语音识别、人机互动、自动测评等技术的应用促使外语学习方式发生巨大变革。非正式数字化学习、智能语言学习系统、混合式学习、数据驱动学习等话题是该领域研究的热点。研究进而总结了新技术的广泛应用推动该领域呈现的发展趋势,并提出对今后相关研究的启示。
文摘Class Ⅲ β-tubulin （Tubb3） is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel bacterial artificial chromosome （BAC） transgenic mouse line that expresses Class Ⅲ β-tubulin fused to mCherry, an improved monomeric red fluorescent protein, for the visualization of microtubules during neuronal development. A BAC containing Tubb3 gene was modified by insertion of mCherry complementary DNA downstream of Tubb3 coding sequence via homologous recombination, mCherry fusion protein was expressed in the nervous system and testis of the transgenic animal, and the fluorescent signal was observed in the neurons that located in the olfactory bulb, cerebral cortex, hippocampal formation, cerebellum, as well as the retina. Besides, Tubb3-mCherry fusion protein mainly distributed in neurites and colocalized with endogenous Class Ⅲ β-tubulin The fusion protein labels Purkinje cell dendrites during cerebellar circuit formation. Therefore, this transgenic line might be a novel tool for scientific community to study neuronal development both in vitro and in vivo.
