本研究旨在探讨肝复胶囊(Gan Fu capsule, GFC)对TGF-1诱导HSC-T6细胞增殖的影响及作用机制。试验采用制备空白血清和GFC含药血清,体外培养HSC-T6细胞24 h后,CCK8法筛选最佳给药血清浓度,采用不同浓度TGF-1诱导HSC-T6细胞,CCK8法筛选最...本研究旨在探讨肝复胶囊(Gan Fu capsule, GFC)对TGF-1诱导HSC-T6细胞增殖的影响及作用机制。试验采用制备空白血清和GFC含药血清,体外培养HSC-T6细胞24 h后,CCK8法筛选最佳给药血清浓度,采用不同浓度TGF-1诱导HSC-T6细胞,CCK8法筛选最佳造模浓度,将细胞分为空白组(CON)、模型组(MOD)、肝复胶囊低剂量组(GFC-L)、肝复胶囊中剂量组(GFC-M)、肝复胶囊高剂量组(GFC-H),采用细胞增殖检测(CCK8)法检测各组细胞增殖情况,酶联免疫吸附试验(ELISA)检测IL-6、IL-1 、TNF-含量,蛋白免疫印记试验(Western blot)检测各细胞中核苷酸结合寡聚化结构域样受体蛋白3(Nod-like receptor protein 3,NLRP3)、凋亡相关斑点样蛋白(Apoptosis associated speck-like protein containing a caspase activating recruitment domain, ASC)、半胱氨酸蛋白酶-1(Cysteinyl aspartate specific proteinase-1,Caspase-1)及-平滑肌动蛋白(Alpha-smooth muscle actin,-SMA)的表达。结果表明,与CON组相比,GFC能抑制HSC-T6细胞的增殖能力,减少HSC分泌IL-6、IL-1 、TNF-含量,降低HSC中NLRP3、ASC、Caspase-1及-SMA的表达量。GFC可能通过调节NLRP3/ASC/Caspase-1信号通路和减少-SMA蛋白表达,从而抑制HSC-T6增殖,起到抗肝纤维化的作用。展开更多
岩体在外部荷载冲击作用下会产生不同频率的信号。首先,通过自制探头的光纤监测系统监测现场岩体受到瞬时冲击荷载前后的应力波信号,并采用鲁棒性局部均值分解(robust local mean decomposition,RLMD)方法,结合快速傅里叶变换对实验得...岩体在外部荷载冲击作用下会产生不同频率的信号。首先,通过自制探头的光纤监测系统监测现场岩体受到瞬时冲击荷载前后的应力波信号,并采用鲁棒性局部均值分解(robust local mean decomposition,RLMD)方法,结合快速傅里叶变换对实验得到的监测信号进行时频分析;然后,通过LS-DYNA软件模拟冲击荷载施加于岩体并产生应力波的过程,并将模拟应力波频率与实验监测应力波频率进行对比;最后,分析了弹性模量和密度发生改变时模拟应力波频率的变化。结果表明:在现场施加冲击荷载后,现场监测所得信号经过频谱分解会出现频率为1500~2300 Hz的多个极大振幅特征信号,与模拟应力波时频分析中获得的2203 Hz的主频率信号基本符合;模拟应力波频率与一维平面应力波推导的频率呈相反的变化趋势。展开更多
Field tests have demonstrated that depressurization with controlled sand production is an effective technique for natural gas hydrate extraction.Variations in depositional environments and processes result in signific...Field tests have demonstrated that depressurization with controlled sand production is an effective technique for natural gas hydrate extraction.Variations in depositional environments and processes result in significant heterogeneity within subsea natural gas hydrate-bearing sediments.However,the influence of permeability heterogeneity on production performance during depressurization with controlled sand production remains inadequately understood.In this study,a multiphase,multi-component mathematical model is developed to simulate depressurization with controlled sand production in methane hydrate-bearing sediments,incorporating geological conditions representative of unconsolidated argillaceous siltstone hydrate deposits in the Shenhu area of the South China Sea.The effects of permeability heterogeneity-specifically,horizontal autocorrelation length and global permeability heterogeneity-on production performance during depressurization with sand production are investigated using geostatistical modeling combined with finite difference method based numerical simulations.Results show that as the horizontal autocorrelation length of permeability distribution increases,cumulative gas production first rises and then declines,reaching its peak at λ_(Dh)=0.1,whereas sand production steadily increases.In addition,higher formation permeability heterogeneity results in increased cumulative gas and sand production,suggesting that greater heterogeneity promotesmethane hydrate decomposition and gas recovery.These findings can offer valuable insights for optimizing future field development of hydrate-bearing sediments by depressurization with controlled sand production.展开更多
Aqueous zinc-ion batteries(AZIBs)are widely used in energy storage devices due to their low cost and environmental sustainability.Nevertheless,the growth of Zn dendrites and the occurrence of side reactions remain sig...Aqueous zinc-ion batteries(AZIBs)are widely used in energy storage devices due to their low cost and environmental sustainability.Nevertheless,the growth of Zn dendrites and the occurrence of side reactions remain significant barriers to the practical application of AZIBs.Here,a hydrophobic and zinc-compatible solid-electrolyte interface layer of poly(dimethylsiloxane)(PDMS)is in situ grafted on the Zn anode surface via spontaneous hydrolytic condens ation reactions.The high viscoelasticity of PDMS and the chemically formed Si-O-Zn bonds synergistically ensure the adaptability and stability of PDMS on Zn anodes.Moreover,the strong hydrophobicity of PDMS shields the direct contact between the Zn anode and the aqueous electrolyte and further optimizes the reversible plating/stripping of Zn.The symmetrical cell assembled with PDMS@Zn anode displays a long lifespan of over 3000 h at 1 mA cm^(-2)for 1 mAh cm^(-2).The PDMS@Zn‖NH_(4)V_(4)O_(10)full cell maintains the specific capacity of 284.8 mAh g^(-1)after 1200 cycles at 1 A g^(-1).Overall,our work sheds new light on the Zn electrodeposition process under the mediation of anode interface,offers sustainability considerations in designing stable Zn metal anodes,as well as provides a facile and viable path for stabilizing Zn anodes to achieve dendrite-free and long lifespan.展开更多
文摘岩体在外部荷载冲击作用下会产生不同频率的信号。首先,通过自制探头的光纤监测系统监测现场岩体受到瞬时冲击荷载前后的应力波信号,并采用鲁棒性局部均值分解(robust local mean decomposition,RLMD)方法,结合快速傅里叶变换对实验得到的监测信号进行时频分析;然后,通过LS-DYNA软件模拟冲击荷载施加于岩体并产生应力波的过程,并将模拟应力波频率与实验监测应力波频率进行对比;最后,分析了弹性模量和密度发生改变时模拟应力波频率的变化。结果表明:在现场施加冲击荷载后,现场监测所得信号经过频谱分解会出现频率为1500~2300 Hz的多个极大振幅特征信号,与模拟应力波时频分析中获得的2203 Hz的主频率信号基本符合;模拟应力波频率与一维平面应力波推导的频率呈相反的变化趋势。
基金funded by the National Key Research and Development Program of China(grant number 2023YFC3009204)the National Natural Science Foundation of China(grant number 52174015).
文摘Field tests have demonstrated that depressurization with controlled sand production is an effective technique for natural gas hydrate extraction.Variations in depositional environments and processes result in significant heterogeneity within subsea natural gas hydrate-bearing sediments.However,the influence of permeability heterogeneity on production performance during depressurization with controlled sand production remains inadequately understood.In this study,a multiphase,multi-component mathematical model is developed to simulate depressurization with controlled sand production in methane hydrate-bearing sediments,incorporating geological conditions representative of unconsolidated argillaceous siltstone hydrate deposits in the Shenhu area of the South China Sea.The effects of permeability heterogeneity-specifically,horizontal autocorrelation length and global permeability heterogeneity-on production performance during depressurization with sand production are investigated using geostatistical modeling combined with finite difference method based numerical simulations.Results show that as the horizontal autocorrelation length of permeability distribution increases,cumulative gas production first rises and then declines,reaching its peak at λ_(Dh)=0.1,whereas sand production steadily increases.In addition,higher formation permeability heterogeneity results in increased cumulative gas and sand production,suggesting that greater heterogeneity promotesmethane hydrate decomposition and gas recovery.These findings can offer valuable insights for optimizing future field development of hydrate-bearing sediments by depressurization with controlled sand production.
基金financially supported by the National Natural Science Foundation of China(Nos.22302066,52477213 and 52174247)the Science and Technology Innovation Program of Hunan Province(Nos.2022RC1088 and 2024RC3220)+1 种基金the Natural Science Foundation of Hunan Province(No.2023JJ40255)the Scientific Research Foundation of Hunan Provincial Education Department(Nos.22B0599 and 23A0442)
文摘Aqueous zinc-ion batteries(AZIBs)are widely used in energy storage devices due to their low cost and environmental sustainability.Nevertheless,the growth of Zn dendrites and the occurrence of side reactions remain significant barriers to the practical application of AZIBs.Here,a hydrophobic and zinc-compatible solid-electrolyte interface layer of poly(dimethylsiloxane)(PDMS)is in situ grafted on the Zn anode surface via spontaneous hydrolytic condens ation reactions.The high viscoelasticity of PDMS and the chemically formed Si-O-Zn bonds synergistically ensure the adaptability and stability of PDMS on Zn anodes.Moreover,the strong hydrophobicity of PDMS shields the direct contact between the Zn anode and the aqueous electrolyte and further optimizes the reversible plating/stripping of Zn.The symmetrical cell assembled with PDMS@Zn anode displays a long lifespan of over 3000 h at 1 mA cm^(-2)for 1 mAh cm^(-2).The PDMS@Zn‖NH_(4)V_(4)O_(10)full cell maintains the specific capacity of 284.8 mAh g^(-1)after 1200 cycles at 1 A g^(-1).Overall,our work sheds new light on the Zn electrodeposition process under the mediation of anode interface,offers sustainability considerations in designing stable Zn metal anodes,as well as provides a facile and viable path for stabilizing Zn anodes to achieve dendrite-free and long lifespan.
