The construction of high density genetic linkage map provides a powerful tool to detect and map quantitative trait loci(QTLs) controlling agronomically important traits. In this study, simple sequence repeat(SSR) mark...The construction of high density genetic linkage map provides a powerful tool to detect and map quantitative trait loci(QTLs) controlling agronomically important traits. In this study, simple sequence repeat(SSR) markers and Illumina 9K i Select single nucleotide polymorphism(SNP) genechip were employed to construct one genetic linkage map of common wheat(Triticum aestivum L.) using 191 recombinant inbred lines(RILs) derived from cross Yu 8679×Jing 411. This map included 1 901 SNP loci and 178 SSR loci, covering 1 659.9 c M and 1 000 marker bins, with an average interval distance of 1.66 c M. A, B and D genomes covered 719.1, 703.5 and 237.3 c M, with an average interval distance of 1.66, 1.45 and 2.9 c M, respectively. Notably, the genetic linkage map covered 20 chromosomes, with the exception of chromosome 5D. Bioinformatics analysis revealed that 1 754(92.27%) of 1 901 mapped SNP loci could be aligned to 1 215 distinct wheat unigenes, among which 1 184(97.4%) were located on o ne single chromosome, and the rest 31(2.6%) were located on 2 to 3 chromosomes. By performing in silico comparison, 214 chromosome deletion bin-mapped expressed sequence tags(ESTs), 1 043 Brachypodium genes and 1 033 rice genes were further added onto the genetic linkage map. This map not only integrated genetic and physical maps, SSR and SNP loci, respectively, but also provided the information of Brachypodium and rice genes corresponding to 1 754 SNP loci. Therefore, it will be a useful tool for comparative genomics analysis, fine mapping of QTL/gene controlling agronomically important traits and marker-assisted selection breeding in wheat.展开更多
Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of ...Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 （3.66%） showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.展开更多
14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significant...14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat （Triticum aestivum L., 2n=6x=42, AABBDD） and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.展开更多
目的观察吸烟对青少年心电图参数T波峰末间期(Tp-e间期)、Tp-e/QT和Tp-e/QTc比值的影响,探讨吸烟与室性心律失常发生风险的相关性。方法选择305例青少年为研究对象,将烟龄≥1年,每日吸烟支数≥3支受试者归为吸烟组(152例),其他归为对照...目的观察吸烟对青少年心电图参数T波峰末间期(Tp-e间期)、Tp-e/QT和Tp-e/QTc比值的影响,探讨吸烟与室性心律失常发生风险的相关性。方法选择305例青少年为研究对象,将烟龄≥1年,每日吸烟支数≥3支受试者归为吸烟组(152例),其他归为对照组(153例)。所有受试者均行常规体表12导联心电图检查获得Tp-e间期、Tp-e/QT和Tp-e/QTc比值数据,比较组间差异。分析每日吸烟量、烟龄、开始吸烟年龄与Tp-e间期、Tp-e/QT和Tp-e/QTc比值相关性。结果吸烟组Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值均高于对照组[(96.1±10.8)ms vs(74.7±9.6)ms,0.25±0.03 vs 0.20±0.03,0.23±0.03 vs 0.18±0.03,P均<0.05]。烟龄6年组Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值高于1~3年组、4~5年组(P均<0.05),每日吸烟量>10支组QT间期、Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值高于3~5支组、5~10支组(P均<0.05),开始吸烟年龄12~13岁组Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值高于13~14岁组、15岁组(P均<0.05)。Spearman相关分析结果示Tp-e/QT比值、Tp-e/QTc比值与开始吸烟年龄呈负相关(rs=-0.251、-0.213,P<0.05)。多重线性回归分析结果示开始吸烟年龄与Tp-e/QT比值、Tp-e/QTc比值关系均呈线性关系(P<0.05)。结论吸烟可导致青少年心电图Tp-e间期、Tp-e/QT、Tp-e/QTc比值增高,开始吸烟年龄越早对心室复极影响越大。展开更多
基金financially supported by the National Natural Science Foundation of China (91435204, 31271710)the National 863 Program of China (2012AA10A309)the Program of Conservation and Sustainable Utilization of Wild Relatives of Crops by the Ministry of Agriculture of China (201003021)
文摘The construction of high density genetic linkage map provides a powerful tool to detect and map quantitative trait loci(QTLs) controlling agronomically important traits. In this study, simple sequence repeat(SSR) markers and Illumina 9K i Select single nucleotide polymorphism(SNP) genechip were employed to construct one genetic linkage map of common wheat(Triticum aestivum L.) using 191 recombinant inbred lines(RILs) derived from cross Yu 8679×Jing 411. This map included 1 901 SNP loci and 178 SSR loci, covering 1 659.9 c M and 1 000 marker bins, with an average interval distance of 1.66 c M. A, B and D genomes covered 719.1, 703.5 and 237.3 c M, with an average interval distance of 1.66, 1.45 and 2.9 c M, respectively. Notably, the genetic linkage map covered 20 chromosomes, with the exception of chromosome 5D. Bioinformatics analysis revealed that 1 754(92.27%) of 1 901 mapped SNP loci could be aligned to 1 215 distinct wheat unigenes, among which 1 184(97.4%) were located on o ne single chromosome, and the rest 31(2.6%) were located on 2 to 3 chromosomes. By performing in silico comparison, 214 chromosome deletion bin-mapped expressed sequence tags(ESTs), 1 043 Brachypodium genes and 1 033 rice genes were further added onto the genetic linkage map. This map not only integrated genetic and physical maps, SSR and SNP loci, respectively, but also provided the information of Brachypodium and rice genes corresponding to 1 754 SNP loci. Therefore, it will be a useful tool for comparative genomics analysis, fine mapping of QTL/gene controlling agronomically important traits and marker-assisted selection breeding in wheat.
基金supported by the National Basic Research Program of China(2007CB109000)the National Science Found for Distinguished Young Scholars, China(30925023)+1 种基金the National Natural Science Foundation of China(30671297)the National High-Tech R&D Program of China(2009AA101102)
文摘Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 （3.66%） showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.
基金financially supported by the National Natural Science Foundation of China(30600392,30871529)the National "863" Program of China(2012AA10A309)
文摘14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat （Triticum aestivum L., 2n=6x=42, AABBDD） and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.
文摘目的观察吸烟对青少年心电图参数T波峰末间期(Tp-e间期)、Tp-e/QT和Tp-e/QTc比值的影响,探讨吸烟与室性心律失常发生风险的相关性。方法选择305例青少年为研究对象,将烟龄≥1年,每日吸烟支数≥3支受试者归为吸烟组(152例),其他归为对照组(153例)。所有受试者均行常规体表12导联心电图检查获得Tp-e间期、Tp-e/QT和Tp-e/QTc比值数据,比较组间差异。分析每日吸烟量、烟龄、开始吸烟年龄与Tp-e间期、Tp-e/QT和Tp-e/QTc比值相关性。结果吸烟组Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值均高于对照组[(96.1±10.8)ms vs(74.7±9.6)ms,0.25±0.03 vs 0.20±0.03,0.23±0.03 vs 0.18±0.03,P均<0.05]。烟龄6年组Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值高于1~3年组、4~5年组(P均<0.05),每日吸烟量>10支组QT间期、Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值高于3~5支组、5~10支组(P均<0.05),开始吸烟年龄12~13岁组Tp-e间期、Tp-e/QT比值、Tp-e/QTc比值高于13~14岁组、15岁组(P均<0.05)。Spearman相关分析结果示Tp-e/QT比值、Tp-e/QTc比值与开始吸烟年龄呈负相关(rs=-0.251、-0.213,P<0.05)。多重线性回归分析结果示开始吸烟年龄与Tp-e/QT比值、Tp-e/QTc比值关系均呈线性关系(P<0.05)。结论吸烟可导致青少年心电图Tp-e间期、Tp-e/QT、Tp-e/QTc比值增高,开始吸烟年龄越早对心室复极影响越大。
