目的克隆千金藤中SjOMT31基因,并对其进行生物信息学分析及体外酶活表征来探讨其功能。方法通过PCR扩增获得SjOMT31的全长序列,并利用生物信息学分析蛋白结构。构建PET-30a-SjOMT31原核表达载体,并将其转入BL21(DE3)大肠杆菌中进行诱导...目的克隆千金藤中SjOMT31基因,并对其进行生物信息学分析及体外酶活表征来探讨其功能。方法通过PCR扩增获得SjOMT31的全长序列,并利用生物信息学分析蛋白结构。构建PET-30a-SjOMT31原核表达载体,并将其转入BL21(DE3)大肠杆菌中进行诱导表达。蛋白纯化后进行体外酶活反应,对其功能进行表征。结果从千金藤中扩增得到SjOMT31全长序列,生信分析发现SjOMT31为稳定亲水性蛋白,无跨膜结构域和信号肽,属于膜外蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)结果分析显示,利用原核表达系统成功诱导获得可溶性SjOMT31蛋白,体外酶促反应检测结果表明,SjOMT31能够催化(S)-去甲乌药碱C6、(S)-乌药碱C7和(S)-金黄紫堇碱及四氢非洲防己碱C2位羟基甲基化。结论成功克隆SjOMT31基因,属于膜外蛋白;同时在大肠杆菌中实现了SjOMT31的异源表达,体外酶功能表征发现SjOMT31能催化苄基异喹啉类生物碱C6、C7和原小檗碱C2位羟基发生甲基化,为其在生物碱代谢中的作用提供了重要依据。展开更多
基于模块化多电平换流器的背靠背柔性直流输电系统(back to back modular multilevel converter based high voltage directcurrent,Back-to-BackMMC-HVDC)可实现区域电网的异步互联,提高电网可靠性。为更好地研究背靠背MMCHVDC接入后...基于模块化多电平换流器的背靠背柔性直流输电系统(back to back modular multilevel converter based high voltage directcurrent,Back-to-BackMMC-HVDC)可实现区域电网的异步互联,提高电网可靠性。为更好地研究背靠背MMCHVDC接入后的交直流系统互耦特性,需建立兼顾区域电网仿真效率与精确模拟MMC-HVDC系统动态特性的交直流混合模型。研究了MMC-HVDC运行原理与控制策略,基于电力系统全数字仿真装置(advanceddigitalpowersystem simulator,ADPSS)搭建含背靠背MMC-HVDC系统的交直流电网机电-电磁混合模型。通过仿真对比,验证了上述MMC-HVDC电磁暂态模型及其控制系统的正确性;基于混合模型进行了交直流电网机电-电磁混合仿真研究,并与对交流大电网进行等值简化后的纯电磁模型进行对比分析。结果表明,与将交流电网等效为理想电压源加等值阻抗的纯电磁暂态模型相比,混合仿真模型可更好地体现交流系统特性,更贴近实际工程,为研究交直流互联电网提供了较好的参考。展开更多
Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T cells.In this study,w...Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T cells.In this study,we aimed to investigate the potential application of RSG in the treatment of psoriasis and elucidate its mechanism of action and material basis.Our findings revealed significant improvements upon administration of RSG in an imiquimod(IMQ)-induced psoriasis model.These improvements were characterized by a remarkable increase in the number of tail scales in mice and a substantial amelioration of skin erythema,ulceration,and flaking.By transcriptome sequencing and T-cell flow sorting assay,we identified notable effects of RSG on the modulation of various cellular processes.Specifically,RSG prominently down-regulated the Th17/Treg ratio in damaged skin tissues and reduced the proportion of G2 phase cells.Furthermore,RSG exhibited a stimulatory effect on the proliferation and differentiation of epithelial cells.Of particular interest,we discovered thatβ-sitosterol,sitostenone,stigmasterol,smiglanin,and cinchonain Ib displayed potent inhibitory effects on the IL-17-mediated inflammatory response in HaCaT cells.In summary,our study highlights the therapeutic potential of RSG in the treatment of psoriasis,attributed to its ability to regulate the Th17/Treg balance.These findings contribute to the development of new indications for RSG and provide a solid theoretical foundation for further exploration in this field.展开更多
文摘目的克隆千金藤中SjOMT31基因,并对其进行生物信息学分析及体外酶活表征来探讨其功能。方法通过PCR扩增获得SjOMT31的全长序列,并利用生物信息学分析蛋白结构。构建PET-30a-SjOMT31原核表达载体,并将其转入BL21(DE3)大肠杆菌中进行诱导表达。蛋白纯化后进行体外酶活反应,对其功能进行表征。结果从千金藤中扩增得到SjOMT31全长序列,生信分析发现SjOMT31为稳定亲水性蛋白,无跨膜结构域和信号肽,属于膜外蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶(sodium dodecyl sulfate-polyacrylamide gel electrophoresis,SDS-PAGE)结果分析显示,利用原核表达系统成功诱导获得可溶性SjOMT31蛋白,体外酶促反应检测结果表明,SjOMT31能够催化(S)-去甲乌药碱C6、(S)-乌药碱C7和(S)-金黄紫堇碱及四氢非洲防己碱C2位羟基甲基化。结论成功克隆SjOMT31基因,属于膜外蛋白;同时在大肠杆菌中实现了SjOMT31的异源表达,体外酶功能表征发现SjOMT31能催化苄基异喹啉类生物碱C6、C7和原小檗碱C2位羟基发生甲基化,为其在生物碱代谢中的作用提供了重要依据。
文摘基于模块化多电平换流器的背靠背柔性直流输电系统(back to back modular multilevel converter based high voltage directcurrent,Back-to-BackMMC-HVDC)可实现区域电网的异步互联,提高电网可靠性。为更好地研究背靠背MMCHVDC接入后的交直流系统互耦特性,需建立兼顾区域电网仿真效率与精确模拟MMC-HVDC系统动态特性的交直流混合模型。研究了MMC-HVDC运行原理与控制策略,基于电力系统全数字仿真装置(advanceddigitalpowersystem simulator,ADPSS)搭建含背靠背MMC-HVDC系统的交直流电网机电-电磁混合模型。通过仿真对比,验证了上述MMC-HVDC电磁暂态模型及其控制系统的正确性;基于混合模型进行了交直流电网机电-电磁混合仿真研究,并与对交流大电网进行等值简化后的纯电磁模型进行对比分析。结果表明,与将交流电网等效为理想电压源加等值阻抗的纯电磁暂态模型相比,混合仿真模型可更好地体现交流系统特性,更贴近实际工程,为研究交直流互联电网提供了较好的参考。
基金supported by the National Natural Science Foundation of China(Nos.82074114,81973562 and 81773995),the Scholar of the 14th batch of“Six Talents Peak”highlevel talent selection(No.SWYY-094)the“Double First-Class”University project(No.CPU2018GY33).
文摘Total glucosides of Rhizoma Smilacis Glabrae(RSG)are selective immunosuppressants that exhibit primary efficacy in the treatment of rheumatoid arthritis through targeted inhibition of activated T cells.In this study,we aimed to investigate the potential application of RSG in the treatment of psoriasis and elucidate its mechanism of action and material basis.Our findings revealed significant improvements upon administration of RSG in an imiquimod(IMQ)-induced psoriasis model.These improvements were characterized by a remarkable increase in the number of tail scales in mice and a substantial amelioration of skin erythema,ulceration,and flaking.By transcriptome sequencing and T-cell flow sorting assay,we identified notable effects of RSG on the modulation of various cellular processes.Specifically,RSG prominently down-regulated the Th17/Treg ratio in damaged skin tissues and reduced the proportion of G2 phase cells.Furthermore,RSG exhibited a stimulatory effect on the proliferation and differentiation of epithelial cells.Of particular interest,we discovered thatβ-sitosterol,sitostenone,stigmasterol,smiglanin,and cinchonain Ib displayed potent inhibitory effects on the IL-17-mediated inflammatory response in HaCaT cells.In summary,our study highlights the therapeutic potential of RSG in the treatment of psoriasis,attributed to its ability to regulate the Th17/Treg balance.These findings contribute to the development of new indications for RSG and provide a solid theoretical foundation for further exploration in this field.
