Background:Cochlear hair cell injury is a common pathological feature of hearing loss.The basic helix-loop-helix family,member e40(Bhlhe40),a gene belonging to the basic helix-loop-helix(bHLH)family,exhibits strong tr...Background:Cochlear hair cell injury is a common pathological feature of hearing loss.The basic helix-loop-helix family,member e40(Bhlhe40),a gene belonging to the basic helix-loop-helix(bHLH)family,exhibits strong transcriptional repression activity.Methods:Oxidative damage,in House Ear Institute-Organ of Corti 1(HEI-OC1)cells,was caused using hydrogen peroxide(H2O2).The Ad-Bhlhe40 particles were constructed to overexpress Bhlhe40 in HEI-OC1 cells.Various assays including cell counting kit-8(CCK-8),terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay(TUNEL),flow cytometry,immunofluorescence,and corresponding commercial kits were employed to investigate the impacts of Bhlhe40 on cell viability,apoptosis,oxidative stress levels,mitochondrial membrane potential and cellular senescence.Additionally,a dual-luciferase reporter assay was performed to confirm the targeting of the histone deacetylases 2(Hdac2)by Bhlhe40.Results:The results revealed that Bhlhe40 was downregulated in H_(2)O_(2)-treated HEI-OC1 cells,but its overexpression improved cell viability and mitigated H_(2)O_(2)-induced oxidative injury in HEI-OC1 cells with increase of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx)activities and decrease of reactive oxygen species(ROS)levels.Besides,overexpression of Bhlhe40 suppressed H_(2)O_(2)-triggered cell senescence,as evidenced by the fact that the upregulation of P53,P21,and P16 in HEI-OC1 cells treated with H2O2 were all alleviated by Bhlhe40 overexpression.And we further verified that overexpression of Bhlhe40 could inhibit the expression of Hdac2,which may be related to the repression of Hdac2 transcription.Conclusion:This study suggests that Bhlhe40 plays a protective role against senescence and oxidative damage in cochlear hair cells exposed to H2O2.展开更多
Economics is a science that studies how the economy grows,so the theory of economic growth is the most important theory of economics.In the real market economy society,people achieve the goal of economic growth throug...Economics is a science that studies how the economy grows,so the theory of economic growth is the most important theory of economics.In the real market economy society,people achieve the goal of economic growth through two kinds of economic activities:production and transaction.Then a correct economic growth theory must be one that can explain both production and transaction economic activities.Just like Newton’s law of universal gravitation in physics,it can explain the motion law of all objects.For a long time,we have been dominated by the western economic growth theory of western mainstream economics.It is not difficult to find that it has a fatal defect,which can only explain production economic activities but not transaction economic activities.So it can’t explain the Chinese economy,and it can’t explain the western economy.The new economic growth theory proposed in this paper makes up for the defects of western economic growth theory,and it is the terminator of western economic growth theory.This is a revolution of new economics to traditional western economics.展开更多
Composite index is always derived with the weighted aggregation of hierarchical components,which is widely utilized to distill intricate and multidimensional matters in economic and business statistics.However,the com...Composite index is always derived with the weighted aggregation of hierarchical components,which is widely utilized to distill intricate and multidimensional matters in economic and business statistics.However,the composite indices always present inevitable anomalies at different levels oriented from the calculation and expression processes of hierarchical components,thereby impairing the precise depiction of specific economic issues.In this paper,we propose VisCI,a visualization framework for anomaly detection and interactive optimization of composite index.First,LSTM-AE model is performed to detect anomalies from the lower level to the higher level of the composite index.Then,a comprehensive array of visual cues is designed to visualize anomalies,such as hierarchy and anomaly visualization.In addition,an interactive operation is provided to ensure accurate and efficient index optimization,mitigating the adverse impact of anomalies on index calculation and representation.Finally,we implement a visualization framework with interactive interfaces,facilitating both anomaly detection and intuitive composite index optimization.Case studies based on real-world datasets and expert interviews are conducted to demonstrate the effectiveness of our VisCI in commodity index anomaly exploration and anomaly optimization.展开更多
基金This research was supported by the Special Fund for Economic and Technological Development of Longgang District,Shenzhen(LGKCYLWS2021000030).
文摘Background:Cochlear hair cell injury is a common pathological feature of hearing loss.The basic helix-loop-helix family,member e40(Bhlhe40),a gene belonging to the basic helix-loop-helix(bHLH)family,exhibits strong transcriptional repression activity.Methods:Oxidative damage,in House Ear Institute-Organ of Corti 1(HEI-OC1)cells,was caused using hydrogen peroxide(H2O2).The Ad-Bhlhe40 particles were constructed to overexpress Bhlhe40 in HEI-OC1 cells.Various assays including cell counting kit-8(CCK-8),terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay(TUNEL),flow cytometry,immunofluorescence,and corresponding commercial kits were employed to investigate the impacts of Bhlhe40 on cell viability,apoptosis,oxidative stress levels,mitochondrial membrane potential and cellular senescence.Additionally,a dual-luciferase reporter assay was performed to confirm the targeting of the histone deacetylases 2(Hdac2)by Bhlhe40.Results:The results revealed that Bhlhe40 was downregulated in H_(2)O_(2)-treated HEI-OC1 cells,but its overexpression improved cell viability and mitigated H_(2)O_(2)-induced oxidative injury in HEI-OC1 cells with increase of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx)activities and decrease of reactive oxygen species(ROS)levels.Besides,overexpression of Bhlhe40 suppressed H_(2)O_(2)-triggered cell senescence,as evidenced by the fact that the upregulation of P53,P21,and P16 in HEI-OC1 cells treated with H2O2 were all alleviated by Bhlhe40 overexpression.And we further verified that overexpression of Bhlhe40 could inhibit the expression of Hdac2,which may be related to the repression of Hdac2 transcription.Conclusion:This study suggests that Bhlhe40 plays a protective role against senescence and oxidative damage in cochlear hair cells exposed to H2O2.
文摘Economics is a science that studies how the economy grows,so the theory of economic growth is the most important theory of economics.In the real market economy society,people achieve the goal of economic growth through two kinds of economic activities:production and transaction.Then a correct economic growth theory must be one that can explain both production and transaction economic activities.Just like Newton’s law of universal gravitation in physics,it can explain the motion law of all objects.For a long time,we have been dominated by the western economic growth theory of western mainstream economics.It is not difficult to find that it has a fatal defect,which can only explain production economic activities but not transaction economic activities.So it can’t explain the Chinese economy,and it can’t explain the western economy.The new economic growth theory proposed in this paper makes up for the defects of western economic growth theory,and it is the terminator of western economic growth theory.This is a revolution of new economics to traditional western economics.
基金National Natural Science Foundation of China(No.62277013,No.62177040)National Statistical Science Research Project(No.2022LY099)+1 种基金Public Welfare Plan Research Project of Zhejiang Provincial Science and Technology Department(No.TGG23H260008)Zhejiang Statistical Science Research Project.
文摘Composite index is always derived with the weighted aggregation of hierarchical components,which is widely utilized to distill intricate and multidimensional matters in economic and business statistics.However,the composite indices always present inevitable anomalies at different levels oriented from the calculation and expression processes of hierarchical components,thereby impairing the precise depiction of specific economic issues.In this paper,we propose VisCI,a visualization framework for anomaly detection and interactive optimization of composite index.First,LSTM-AE model is performed to detect anomalies from the lower level to the higher level of the composite index.Then,a comprehensive array of visual cues is designed to visualize anomalies,such as hierarchy and anomaly visualization.In addition,an interactive operation is provided to ensure accurate and efficient index optimization,mitigating the adverse impact of anomalies on index calculation and representation.Finally,we implement a visualization framework with interactive interfaces,facilitating both anomaly detection and intuitive composite index optimization.Case studies based on real-world datasets and expert interviews are conducted to demonstrate the effectiveness of our VisCI in commodity index anomaly exploration and anomaly optimization.
