The rising prevalence of metabolic syndrome(MetS),such as obesity and its hepatic complication,and non-alcoholic fatty liver disease(NAFLD),necessitates safe,effective,and protective interventions.Natural products,suc...The rising prevalence of metabolic syndrome(MetS),such as obesity and its hepatic complication,and non-alcoholic fatty liver disease(NAFLD),necessitates safe,effective,and protective interventions.Natural products,such as carotenoids,including bixin derived from annatto seeds,have emerged as promising candidates due to their multifaceted pharmacological properties.This study aimed to characterize the edible food pigment bixin and other co-existing bioactives in acetone-and ethyl lactate-extracts of Bixa Orellana L.seeds,followed by their anti-adipogenic and anti-steatotic assessments using in-vitro models of obesity and NAFLD.LC-MS analysis revealed the presence of various phytochemicals in the bixin rich solvent extracts.In vitro studies demonstrated differential and significant anti-adipogenic and anti-steatotic effects of bixin(pure pigment)and solvent extracts of annatto seeds(P<0.05).The LC-MS profiling of annatto seed extracts revealed the presence of bixin and several bixinoids.Other key phytochemicals that were identified were eicosatrienoic acid,geranylgeraniol,hypolatein,δ-tocotrienol,caffeoyl acid derivative,and zeaxanthin,which were differentially abundant in each solvent extract.Bixin and coexisting bioactives in the annatto seed extracts demonstrated significant anti-oxidant,anti-inflammatory,and anti-lipidemic effects(P<0.05)in the present in vitro MetS models.Further studies may be directed toward evaluating the nutraceutical potential of bixin in combination with the implicated phytochemicals in the extracts for treating metabolic disorders.展开更多
With the growing demand for nutraceuticals,there is renewed interest in tapping different resources,preferably from non-animal sources.This review discusses two major nutraceuticals,vitamin D and essential fatty acids...With the growing demand for nutraceuticals,there is renewed interest in tapping different resources,preferably from non-animal sources.This review discusses two major nutraceuticals,vitamin D and essential fatty acids that have documented health-promoting and disease-preventing benefits.The focus here is on the plant sources of two lipid-based nutraceuticals-vitamin D and stearidonic acid.To this end,the biochemical mechanisms have been discussed for the biosynthesis of these nutrients in plants,and compared with their other sources for potential targeting,using biotechnological and metabolic engineering approaches towards large scale production of these nutraceuticals.Minimizing the dependence on animal sources of nutraceuticals and reducing the extensive organic solvent based extraction procedures,are proposed towards environmentally friendly food production and sustainable scale up.展开更多
基金supported by a research grant from the Indian Council of Medical Research(ICMR),New Delhi(Grant No.52/13/2022-BIO/BMS).
文摘The rising prevalence of metabolic syndrome(MetS),such as obesity and its hepatic complication,and non-alcoholic fatty liver disease(NAFLD),necessitates safe,effective,and protective interventions.Natural products,such as carotenoids,including bixin derived from annatto seeds,have emerged as promising candidates due to their multifaceted pharmacological properties.This study aimed to characterize the edible food pigment bixin and other co-existing bioactives in acetone-and ethyl lactate-extracts of Bixa Orellana L.seeds,followed by their anti-adipogenic and anti-steatotic assessments using in-vitro models of obesity and NAFLD.LC-MS analysis revealed the presence of various phytochemicals in the bixin rich solvent extracts.In vitro studies demonstrated differential and significant anti-adipogenic and anti-steatotic effects of bixin(pure pigment)and solvent extracts of annatto seeds(P<0.05).The LC-MS profiling of annatto seed extracts revealed the presence of bixin and several bixinoids.Other key phytochemicals that were identified were eicosatrienoic acid,geranylgeraniol,hypolatein,δ-tocotrienol,caffeoyl acid derivative,and zeaxanthin,which were differentially abundant in each solvent extract.Bixin and coexisting bioactives in the annatto seed extracts demonstrated significant anti-oxidant,anti-inflammatory,and anti-lipidemic effects(P<0.05)in the present in vitro MetS models.Further studies may be directed toward evaluating the nutraceutical potential of bixin in combination with the implicated phytochemicals in the extracts for treating metabolic disorders.
基金the Institute Fellowship from BITS,PilaniICMR,New Delhi for the research grant(Grant number 52/13/2022-BIO/BMS)the administration of Birla Institute of Technology and Science(BITS),Pilani-Pilani Campus for their support and encouragement.
文摘With the growing demand for nutraceuticals,there is renewed interest in tapping different resources,preferably from non-animal sources.This review discusses two major nutraceuticals,vitamin D and essential fatty acids that have documented health-promoting and disease-preventing benefits.The focus here is on the plant sources of two lipid-based nutraceuticals-vitamin D and stearidonic acid.To this end,the biochemical mechanisms have been discussed for the biosynthesis of these nutrients in plants,and compared with their other sources for potential targeting,using biotechnological and metabolic engineering approaches towards large scale production of these nutraceuticals.Minimizing the dependence on animal sources of nutraceuticals and reducing the extensive organic solvent based extraction procedures,are proposed towards environmentally friendly food production and sustainable scale up.
