The authors regret the following clerical and typographical errors in the published version.The errors and the corrections are shown below.These corrections do not change the presented results,their inter-pretation an...The authors regret the following clerical and typographical errors in the published version.The errors and the corrections are shown below.These corrections do not change the presented results,their inter-pretation and the conclusions of the article.The authors would like to apologise for their oversight,which inadvertently allowed these clerical and typing errors to appear in the published version.Any inconvenience caused is regretted.展开更多
The authors regret the following clerical and typographical errors in the published version.The errors and the corrections are shown below.These corrections do not change the presented results,their inter-pretation an...The authors regret the following clerical and typographical errors in the published version.The errors and the corrections are shown below.These corrections do not change the presented results,their inter-pretation and the conclusions of the article.The authors would like to apologise for their oversight,which inadvertently allowed these clerical and typing errors to appear in the published version.Any inconvenience caused is regretted.展开更多
Pesticide exposure has been associated with various health conditions like asthma,Parkinson’s disease,Alzheimer’s disease,genetic disorders,epigenetic changes,oxidative stress,and hormonal disruption.Recent evidence...Pesticide exposure has been associated with various health conditions like asthma,Parkinson’s disease,Alzheimer’s disease,genetic disorders,epigenetic changes,oxidative stress,and hormonal disruption.Recent evidence suggests that infants are more vulnerable to pesticides(such as rotenone)than adults.Although the disease has already been clinically characterized,no treatment has yet been developed to improve pathology or prevent developmental toxicity.Therefore,the purpose of this study was to investigate whether thymoquinone(TQ)from Nigella sativa seeds could ameliorate rotenone(ROT)-induced developmental damage in both Drosophila melanogaster ovary and third instar larvae.Female adult wild-type flies and third instar larvae(72±2 h after egg laying)were exposed to ROT and/or TQ through food for 48 and 24 h respectively.Results indicate that coexposure of TQ inhibits ROT-induced increased oxidative stress and alleviates abnormal antioxidant enzyme activities.Further,TQ alters developmental gene expression(Bacoid,Oscar,Nanos,and Gurken),impedes mitochondrial dysfunction,and reduces apoptosis in ROT induced ovary.In addition,TQ changes ROT induced abnormal crawling behaviour,cholinergic dysfunction,and dopamine depletion to normal levels in third instar larvae.Also,TQ reduces pupation and emergence time in ROT induced third instar larvae.The above findings demonstrate that ROT exposure during early developmental stages is inevitable,and TQ supplementation prevents developmental toxicity in the Drosophila ovary and third instar larvae.A diet enriched TQ provides a ray of hope in preventing ROT induced developmental toxicity.展开更多
The high-fat diet(HFD)causes cardiac and hepatic injury by inducing metabolic disturbances like oxidative stress,dyslipidemia,steatosis,endothelial dysfunction,angiogenesis,altered organ function parameters,inflammato...The high-fat diet(HFD)causes cardiac and hepatic injury by inducing metabolic disturbances like oxidative stress,dyslipidemia,steatosis,endothelial dysfunction,angiogenesis,altered organ function parameters,inflammatory mediators,and apoptosis.The present study evaluated bioactive compounds rich biomass of microalga Nannochloropsis sp.for its possible protective effect and associated mechanisms against HFD induced hepatic and cardiac metabolic disturbances in a mice model.Supplementation of HFD with 15%of Nannochloropsis sp.biomass significantly alleviated the cardiac and hepatic metabolic disturbances.Supplementation countered the body weight gain,modulated oxidative stress and dyslipidemia,improved cardiovascular risk indices,and attenuated the organ/tissue injury markers AST,ALT,CKMB,and LDH.Histology studies showed that supplementation effectively reduced the HFD induced steatosis,hypertrophy,fibrosis,and necrosis in the liver and heart tissues.Supplementation significantly suppressed the elevated mRNA expression levels of PPAR-γand CD36,thereby protecting the organs against steatosis.Furthermore,the supplementation significantly alleviated the HFD induced perturbations in inflammation and apoptosis by suppressing the elevated caspase-3 and caspase-9 activity and protein expression of NF-κB,TNF-α,COX-2,and iNOS while upregulating the IL-10 protein expression.Supplementation also significantly attenuated the endothelial dysfunction and angiogenesis-associated organ remodelling by downregulating the increased mRNA expression levels of ICAM-1,VCAM-1,and VEGF-A.The study suggests the potential of Nannochloropsis sp.biomass as a functional dietary supplement for the alleviation of HFD induced cardiac and hepatic metabolic disturbances.展开更多
文摘The authors regret the following clerical and typographical errors in the published version.The errors and the corrections are shown below.These corrections do not change the presented results,their inter-pretation and the conclusions of the article.The authors would like to apologise for their oversight,which inadvertently allowed these clerical and typing errors to appear in the published version.Any inconvenience caused is regretted.
文摘The authors regret the following clerical and typographical errors in the published version.The errors and the corrections are shown below.These corrections do not change the presented results,their inter-pretation and the conclusions of the article.The authors would like to apologise for their oversight,which inadvertently allowed these clerical and typing errors to appear in the published version.Any inconvenience caused is regretted.
基金the Indian Council of Medical Research(ICMR),Government of India,for awarding the Senior Research FellowshipDirectorate of Minorities,Government of Karnataka,India,for Junior Research Fellowship[Award No.DOM/FELLOWSHIP/CR-41/2017-18].
文摘Pesticide exposure has been associated with various health conditions like asthma,Parkinson’s disease,Alzheimer’s disease,genetic disorders,epigenetic changes,oxidative stress,and hormonal disruption.Recent evidence suggests that infants are more vulnerable to pesticides(such as rotenone)than adults.Although the disease has already been clinically characterized,no treatment has yet been developed to improve pathology or prevent developmental toxicity.Therefore,the purpose of this study was to investigate whether thymoquinone(TQ)from Nigella sativa seeds could ameliorate rotenone(ROT)-induced developmental damage in both Drosophila melanogaster ovary and third instar larvae.Female adult wild-type flies and third instar larvae(72±2 h after egg laying)were exposed to ROT and/or TQ through food for 48 and 24 h respectively.Results indicate that coexposure of TQ inhibits ROT-induced increased oxidative stress and alleviates abnormal antioxidant enzyme activities.Further,TQ alters developmental gene expression(Bacoid,Oscar,Nanos,and Gurken),impedes mitochondrial dysfunction,and reduces apoptosis in ROT induced ovary.In addition,TQ changes ROT induced abnormal crawling behaviour,cholinergic dysfunction,and dopamine depletion to normal levels in third instar larvae.Also,TQ reduces pupation and emergence time in ROT induced third instar larvae.The above findings demonstrate that ROT exposure during early developmental stages is inevitable,and TQ supplementation prevents developmental toxicity in the Drosophila ovary and third instar larvae.A diet enriched TQ provides a ray of hope in preventing ROT induced developmental toxicity.
基金Chris Cherita acknowledges the Indian Council of Medical Research(ICMR),Government of India,for the award of a Senior Research Fellowship[Award No.3/1/2/95/2018-Nut]。
文摘The high-fat diet(HFD)causes cardiac and hepatic injury by inducing metabolic disturbances like oxidative stress,dyslipidemia,steatosis,endothelial dysfunction,angiogenesis,altered organ function parameters,inflammatory mediators,and apoptosis.The present study evaluated bioactive compounds rich biomass of microalga Nannochloropsis sp.for its possible protective effect and associated mechanisms against HFD induced hepatic and cardiac metabolic disturbances in a mice model.Supplementation of HFD with 15%of Nannochloropsis sp.biomass significantly alleviated the cardiac and hepatic metabolic disturbances.Supplementation countered the body weight gain,modulated oxidative stress and dyslipidemia,improved cardiovascular risk indices,and attenuated the organ/tissue injury markers AST,ALT,CKMB,and LDH.Histology studies showed that supplementation effectively reduced the HFD induced steatosis,hypertrophy,fibrosis,and necrosis in the liver and heart tissues.Supplementation significantly suppressed the elevated mRNA expression levels of PPAR-γand CD36,thereby protecting the organs against steatosis.Furthermore,the supplementation significantly alleviated the HFD induced perturbations in inflammation and apoptosis by suppressing the elevated caspase-3 and caspase-9 activity and protein expression of NF-κB,TNF-α,COX-2,and iNOS while upregulating the IL-10 protein expression.Supplementation also significantly attenuated the endothelial dysfunction and angiogenesis-associated organ remodelling by downregulating the increased mRNA expression levels of ICAM-1,VCAM-1,and VEGF-A.The study suggests the potential of Nannochloropsis sp.biomass as a functional dietary supplement for the alleviation of HFD induced cardiac and hepatic metabolic disturbances.
