A simple and reproducible biosynthetic method was employed to synthesize iron and silver nanoparticles which resulted in monodispersed nanoparticles of high concentration.The iron oxide nanoparticles has been widely f...A simple and reproducible biosynthetic method was employed to synthesize iron and silver nanoparticles which resulted in monodispersed nanoparticles of high concentration.The iron oxide nanoparticles has been widely favored because of low cytotoxicity,biodegradable and reactive surface that can be modified with biocompatible coatings.Silver nanoparticles have been a potent antibacterial,antifungal,anti-viral and anti-inflammatory agent.The reaction process was simple,eco-friendly,inexpensive and easy to handle.Green and chemical methods were employed to synthesize iron and silver nanoparticles.A microbial route to synthesize iron and silver nanoparticles by the fungal strain Fusarium oxysporum sp.and Actinomycetes sp.was done simultaneously.Production of nanoparticles using fungi has some advantages over other organisms as it is easy to handle and require simple raw materials.The obtained iron and silver nanoparticles were characterized by UV-vis spectroscopy,Fourier transform infrared spectroscopy(FTIR)and the morphology of prepared nanoparticles was confirmed by Transmission electron microscopy(TEM).TEM images of Iron nanoparticles synthesized by Fusarium oxysporum sp.showed 20-40 nm sized particles.These particles exhibited maximum antibacterial activity against Bacillus,E.coli and Staphylococcus sps.TEM images of biosynthesized silver nanoparticles were of smaller size(10-20 nm).The microbially synthesized silver nanoparticles using Actinomycetes were found to be highly toxic against different human pathogens due to the smaller size and due to the presence of antibiotic components available on them.The mechanism of antimicrobial property of nanoparticle lies with the fact that the extremely small size means a large surface area relative to the volume,which effectively covers the microorganisms and reduce oxygen supply for respiration.It was found that silver nanoparticles synthesized by the microbial route have a greater antibacterial activity.展开更多
Carbon quantum dots(CQDs)have a size of 10 nm(or less),with lots of biomedical advantages,creating huge excitement in different research fields.The aim of this study includes an eco-friendly synthesis of biogenic CQDs...Carbon quantum dots(CQDs)have a size of 10 nm(or less),with lots of biomedical advantages,creating huge excitement in different research fields.The aim of this study includes an eco-friendly synthesis of biogenic CQDs from grape(Vitis vinifera)seeds,identifying the characteristics and assessing its anti-diabetic as well as anti-microbial activity.CQDs are prepared by the pyrolysis method.Synthesized CQDs were confirmed by ultraviolet(UV)-visible(Vis)spectrophotometer,and the characterization study was done by X-ray diffractometer,photoluminescence spectroscopy,Fourier transform infra-red spectroscopy,and transmission electron microscopy with selected area electron diffraction(SAED).Anti-diabetic activity of CQDs was analyzed by in vitroα-glycosidase,α-amylase inhibition assays,and glucose uptake studies.The anti-bacterial activity of CQDs was analyzed by anti-microbial assay technique against Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,and Streptococcus mutans.The results showed that the CQDs synthesized from a natural source like grape seeds,were amorphous in nature,the average particle size was 4 nm,and they contain functional groups like carboxyl and hydroxyl.Subsequently,it showed that the sp2 domains also produce green fluorescence.The anti-diabetic experimental method revealed that the CQDs enhance glucose uptake and inhibit carbohydrate hydrolyzing enzymes.CQDs also exhibit anti-bacterial properties against both Gram-positive and Gram-negative bacteria,according to their antimicrobial impact.Due to their small size and higher activity,CQDs will become strong anti-diabetic agents as well as anti-bacterial ones.展开更多
文摘A simple and reproducible biosynthetic method was employed to synthesize iron and silver nanoparticles which resulted in monodispersed nanoparticles of high concentration.The iron oxide nanoparticles has been widely favored because of low cytotoxicity,biodegradable and reactive surface that can be modified with biocompatible coatings.Silver nanoparticles have been a potent antibacterial,antifungal,anti-viral and anti-inflammatory agent.The reaction process was simple,eco-friendly,inexpensive and easy to handle.Green and chemical methods were employed to synthesize iron and silver nanoparticles.A microbial route to synthesize iron and silver nanoparticles by the fungal strain Fusarium oxysporum sp.and Actinomycetes sp.was done simultaneously.Production of nanoparticles using fungi has some advantages over other organisms as it is easy to handle and require simple raw materials.The obtained iron and silver nanoparticles were characterized by UV-vis spectroscopy,Fourier transform infrared spectroscopy(FTIR)and the morphology of prepared nanoparticles was confirmed by Transmission electron microscopy(TEM).TEM images of Iron nanoparticles synthesized by Fusarium oxysporum sp.showed 20-40 nm sized particles.These particles exhibited maximum antibacterial activity against Bacillus,E.coli and Staphylococcus sps.TEM images of biosynthesized silver nanoparticles were of smaller size(10-20 nm).The microbially synthesized silver nanoparticles using Actinomycetes were found to be highly toxic against different human pathogens due to the smaller size and due to the presence of antibiotic components available on them.The mechanism of antimicrobial property of nanoparticle lies with the fact that the extremely small size means a large surface area relative to the volume,which effectively covers the microorganisms and reduce oxygen supply for respiration.It was found that silver nanoparticles synthesized by the microbial route have a greater antibacterial activity.
文摘Carbon quantum dots(CQDs)have a size of 10 nm(or less),with lots of biomedical advantages,creating huge excitement in different research fields.The aim of this study includes an eco-friendly synthesis of biogenic CQDs from grape(Vitis vinifera)seeds,identifying the characteristics and assessing its anti-diabetic as well as anti-microbial activity.CQDs are prepared by the pyrolysis method.Synthesized CQDs were confirmed by ultraviolet(UV)-visible(Vis)spectrophotometer,and the characterization study was done by X-ray diffractometer,photoluminescence spectroscopy,Fourier transform infra-red spectroscopy,and transmission electron microscopy with selected area electron diffraction(SAED).Anti-diabetic activity of CQDs was analyzed by in vitroα-glycosidase,α-amylase inhibition assays,and glucose uptake studies.The anti-bacterial activity of CQDs was analyzed by anti-microbial assay technique against Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,and Streptococcus mutans.The results showed that the CQDs synthesized from a natural source like grape seeds,were amorphous in nature,the average particle size was 4 nm,and they contain functional groups like carboxyl and hydroxyl.Subsequently,it showed that the sp2 domains also produce green fluorescence.The anti-diabetic experimental method revealed that the CQDs enhance glucose uptake and inhibit carbohydrate hydrolyzing enzymes.CQDs also exhibit anti-bacterial properties against both Gram-positive and Gram-negative bacteria,according to their antimicrobial impact.Due to their small size and higher activity,CQDs will become strong anti-diabetic agents as well as anti-bacterial ones.
