Objective:To investigate the anti-biofilm and anti-bacterial activity of Junceella juncea(J.juncea)against biofilm forming pathogenic strains.Methods:Gorgonians were extraeted with methanol and analysed with fourier t...Objective:To investigate the anti-biofilm and anti-bacterial activity of Junceella juncea(J.juncea)against biofilm forming pathogenic strains.Methods:Gorgonians were extraeted with methanol and analysed with fourier transform infrared spectroscopy.Biofilm forming pathogens were identified by Congo red agar supplemented with sucrose.A quantitative spectrophotometric method was used to monitor in vitro biofilm reduction by microtitre plate assay.Anti-bacterial activity of methanolic gorgonian extract(MGE)was carried out by disc diffusion method followed by calculating the percentage of increase with crude methanol(CM).Results:The presence of active functional group was exemplified by FT-IR spectroscopy.Dry,black,crystalline colonies confirm the production of extracellular polymeric substances responsible for biofilm formation in Congo red agar.MGE exhibited potential anti-biofilm activity against all tested bacterial strains.The anti-bacterial activity of methanolic extract was comparably higher in Salmonella typhii followed by Escherichia colt,Vibrio cholerae and Shigella flexneri.The overall percentage of increase was higher by 50.2%to CM.Conclusions:To conclude,anti-biofilm and anti-bacterial efficacy of J.juncea is impressive over biofilm producing pathogens and are good source for novel anti-bacterial compounds.展开更多
Synthesis of nanomaterials by biological approach is innovative,cheaper and environmental friendly and requires less-labor.In this regard,the present study focused on the synthesis of silver nanoparticles from the ext...Synthesis of nanomaterials by biological approach is innovative,cheaper and environmental friendly and requires less-labor.In this regard,the present study focused on the synthesis of silver nanoparticles from the extract of Sargassum tenerrimum.Synthesized silver nanoparticles were well-characterized by UV-Visible Spectroscopy(UV-Vis),Fourier-Transform Infra-red Spectroscopy(FT-IR),Transmission Electron Microscopy analysis(TEM)and Dynamic Light Scattering(DLS).It was found that spherical shaped nanoparticles of size 20 nm were found in TEM analysis and showed effective anti-bacterial activity against standard reference strains.Altogether,extracts from seaweed were screened for phytochemicals followed by FT-IR prediction to reveal chemical functional groups present.The results showed that the anti-bacterial activity of silver nanoparticles was comparably higher than the phytochemicals present.Therefore,the present study elucidates silver nanoparticles can play a vital role in nano-based therapy in future.展开更多
基金Supported by DST-NRDMS,Government of India(grant No.041594/F3/2008/dt.08.12.2010)
文摘Objective:To investigate the anti-biofilm and anti-bacterial activity of Junceella juncea(J.juncea)against biofilm forming pathogenic strains.Methods:Gorgonians were extraeted with methanol and analysed with fourier transform infrared spectroscopy.Biofilm forming pathogens were identified by Congo red agar supplemented with sucrose.A quantitative spectrophotometric method was used to monitor in vitro biofilm reduction by microtitre plate assay.Anti-bacterial activity of methanolic gorgonian extract(MGE)was carried out by disc diffusion method followed by calculating the percentage of increase with crude methanol(CM).Results:The presence of active functional group was exemplified by FT-IR spectroscopy.Dry,black,crystalline colonies confirm the production of extracellular polymeric substances responsible for biofilm formation in Congo red agar.MGE exhibited potential anti-biofilm activity against all tested bacterial strains.The anti-bacterial activity of methanolic extract was comparably higher in Salmonella typhii followed by Escherichia colt,Vibrio cholerae and Shigella flexneri.The overall percentage of increase was higher by 50.2%to CM.Conclusions:To conclude,anti-biofilm and anti-bacterial efficacy of J.juncea is impressive over biofilm producing pathogens and are good source for novel anti-bacterial compounds.
文摘Synthesis of nanomaterials by biological approach is innovative,cheaper and environmental friendly and requires less-labor.In this regard,the present study focused on the synthesis of silver nanoparticles from the extract of Sargassum tenerrimum.Synthesized silver nanoparticles were well-characterized by UV-Visible Spectroscopy(UV-Vis),Fourier-Transform Infra-red Spectroscopy(FT-IR),Transmission Electron Microscopy analysis(TEM)and Dynamic Light Scattering(DLS).It was found that spherical shaped nanoparticles of size 20 nm were found in TEM analysis and showed effective anti-bacterial activity against standard reference strains.Altogether,extracts from seaweed were screened for phytochemicals followed by FT-IR prediction to reveal chemical functional groups present.The results showed that the anti-bacterial activity of silver nanoparticles was comparably higher than the phytochemicals present.Therefore,the present study elucidates silver nanoparticles can play a vital role in nano-based therapy in future.
