Introduction:Antibiotic resistance is a major global health challenge that disproportionately affects low-resource countries,particularly those in West Africa.E.coli,a major pathogen in childhood diarrhea,is both a pr...Introduction:Antibiotic resistance is a major global health challenge that disproportionately affects low-resource countries,particularly those in West Africa.E.coli,a major pathogen in childhood diarrhea,is both a prominent infectious agent and a reservoir of resistance genes,including resistance to lastresort antibiotics,such as carbapenems.Methodology:The study focused on 98 clinical E.coli isolates collected from stool samples of patients in a hospital setting in Bamako.The analyses included screening for DEC-specific virulence genes,detection of resistance genes across various classes of antibiotics(e.g.,beta-lactams,carbapenems,fluoroquinolones),and identification of class 1,2,and 3 integrons.The bla NDM gene was sequenced to identify mutations associated with carbapenem resistance.Results:Among the isolates,85.7%carried at least one virulence gene.Of these,half involved co-infections,commonly combining EPEC,EAEC,and ETEC strains.Regarding antibiotic resistance,94.9%of isolates harbored at least one resistance gene,and 50%were multidrug-resistant.The most frequently detected genes were bla TEM,qnrS1,and aphA3.Class 2 integrons were significantly associated with multidrug resistance(p=0.01).Sequencing of the bla NDM gene revealed point mutations likely to affect protein function,suggesting an evolution toward increased resistance to carbapenems.Conclusion:The high prevalence of multidrug-resistant diarrheagenic E.coli strains in this study highlights the local antibiotic pressure and the serious health threat it represents.This study shows that the newβ-lactamase bla NDM gene has disseminated in the hospital environment of Bamako.It should be noted that this will become a major challenge for clinicians.展开更多
文摘Introduction:Antibiotic resistance is a major global health challenge that disproportionately affects low-resource countries,particularly those in West Africa.E.coli,a major pathogen in childhood diarrhea,is both a prominent infectious agent and a reservoir of resistance genes,including resistance to lastresort antibiotics,such as carbapenems.Methodology:The study focused on 98 clinical E.coli isolates collected from stool samples of patients in a hospital setting in Bamako.The analyses included screening for DEC-specific virulence genes,detection of resistance genes across various classes of antibiotics(e.g.,beta-lactams,carbapenems,fluoroquinolones),and identification of class 1,2,and 3 integrons.The bla NDM gene was sequenced to identify mutations associated with carbapenem resistance.Results:Among the isolates,85.7%carried at least one virulence gene.Of these,half involved co-infections,commonly combining EPEC,EAEC,and ETEC strains.Regarding antibiotic resistance,94.9%of isolates harbored at least one resistance gene,and 50%were multidrug-resistant.The most frequently detected genes were bla TEM,qnrS1,and aphA3.Class 2 integrons were significantly associated with multidrug resistance(p=0.01).Sequencing of the bla NDM gene revealed point mutations likely to affect protein function,suggesting an evolution toward increased resistance to carbapenems.Conclusion:The high prevalence of multidrug-resistant diarrheagenic E.coli strains in this study highlights the local antibiotic pressure and the serious health threat it represents.This study shows that the newβ-lactamase bla NDM gene has disseminated in the hospital environment of Bamako.It should be noted that this will become a major challenge for clinicians.
