Background:Biotransformation of quinic acid to shikimic acid was attempted using whole cells of Bacillus megaterium as a biocatalyst.Results:Physico-chemical parameters such as temperature(37℃),pH(7.0),agitation(200 ...Background:Biotransformation of quinic acid to shikimic acid was attempted using whole cells of Bacillus megaterium as a biocatalyst.Results:Physico-chemical parameters such as temperature(37℃),pH(7.0),agitation(200 rpm),substrate(5 mM)and cell mass concentrations(200 kg/m^(3))and reaction time(3 h)were found optimum to enhance the bioconversion.Maximum conversion(89%)of quinic acid to shikimic acid was achieved using the above optimized parameters.Shikimic acid was extracted from the reaction mixture by a pH-dependent method and maximum recovery(76%)was obtained with petroleum ether.Conclusions:Biotransformation of quinic acid to shikimic acid seems to be a better alternative over its fermentative production.展开更多
文摘Background:Biotransformation of quinic acid to shikimic acid was attempted using whole cells of Bacillus megaterium as a biocatalyst.Results:Physico-chemical parameters such as temperature(37℃),pH(7.0),agitation(200 rpm),substrate(5 mM)and cell mass concentrations(200 kg/m^(3))and reaction time(3 h)were found optimum to enhance the bioconversion.Maximum conversion(89%)of quinic acid to shikimic acid was achieved using the above optimized parameters.Shikimic acid was extracted from the reaction mixture by a pH-dependent method and maximum recovery(76%)was obtained with petroleum ether.Conclusions:Biotransformation of quinic acid to shikimic acid seems to be a better alternative over its fermentative production.
