Crops face increased risk from heat stress due to climate change.Potato(Solanum tuberosum L.)tubers grown in hot summers often have defects including pre-harvest sprouting(“heat sprouts”).We have used 18 potato cult...Crops face increased risk from heat stress due to climate change.Potato(Solanum tuberosum L.)tubers grown in hot summers often have defects including pre-harvest sprouting(“heat sprouts”).We have used 18 potato cultivars to investigate whether heat stress(HS)conditions alone could cause heat sprouting and dormancy changes in tubers.We also examined transcriptomic responses of potato to HS and whether these responses are like those induced by postharvest sprouting.We demonstrated that HS alone caused heat sprouts and shortened postharvest dormancy period,heat-sprouted tubers became dormant after harvest,and cultivars varied substantially for producing heat spouts but there was no clear association with cultivar maturity earliness.Cultivar Innovator did not show any heat sprouts and still had long dormancy.Dormancy-associated genes(DOG1 and SLP)were downregulated in HS tubers like in postharvest sprouting tubers.We have identified 1201 differentially expressed genes,14 enriched GO terms and 12 enriched KEGG pathways in response to HS in growing tubers of‘Russet Burbank’.Transcriptomic response of‘Russet Burbank’to HS showed significant similarities to that of postharvest non-HS sprouted tubers.Gibberellin biosynthesis pathway was enriched in heat-stressed tubers and was likely involved in heat sprouting and dormancy release.Heat sprouting and postharvest sprouting shared common candidate genes and had significant similarity in gene expression.Our study has significance for selecting potato cultivars for farming,planning storage and utilization of heat-stressed tubers,identifying sprouting-related genes,understanding heat-stress biology,and breeding heat-tolerant potato cultivars,especially for sustainable potato production under climate change.展开更多
基金This research was mainly supported by the National Natural Science Foundation of China(Nos.31860399,31560413 to Huaijun Si)A-base funding of Agriculture and Agri-Food Canada(AAFC),Government of Canada(to Xiu-Qing Li)+3 种基金We thank the China Scholarship Council for its support to Guodong Zhang and Ruimin Tang to work in AAFC under Xiu-Qing Li’s supervisionWe also thank the funding supports from the Natural Science Foundation of Jiangsu Province(No.BK20180519 to Qing Yang)the Key Research Projects Program of Higher Education Institutes in Henan Provinces(21A210029 to Suyan Niu)the funding from the Natural Sciences and Engineering Research Council of Canada Discovery Grant(RGPIN 2017-04589 to Om P.Rajora)。
文摘Crops face increased risk from heat stress due to climate change.Potato(Solanum tuberosum L.)tubers grown in hot summers often have defects including pre-harvest sprouting(“heat sprouts”).We have used 18 potato cultivars to investigate whether heat stress(HS)conditions alone could cause heat sprouting and dormancy changes in tubers.We also examined transcriptomic responses of potato to HS and whether these responses are like those induced by postharvest sprouting.We demonstrated that HS alone caused heat sprouts and shortened postharvest dormancy period,heat-sprouted tubers became dormant after harvest,and cultivars varied substantially for producing heat spouts but there was no clear association with cultivar maturity earliness.Cultivar Innovator did not show any heat sprouts and still had long dormancy.Dormancy-associated genes(DOG1 and SLP)were downregulated in HS tubers like in postharvest sprouting tubers.We have identified 1201 differentially expressed genes,14 enriched GO terms and 12 enriched KEGG pathways in response to HS in growing tubers of‘Russet Burbank’.Transcriptomic response of‘Russet Burbank’to HS showed significant similarities to that of postharvest non-HS sprouted tubers.Gibberellin biosynthesis pathway was enriched in heat-stressed tubers and was likely involved in heat sprouting and dormancy release.Heat sprouting and postharvest sprouting shared common candidate genes and had significant similarity in gene expression.Our study has significance for selecting potato cultivars for farming,planning storage and utilization of heat-stressed tubers,identifying sprouting-related genes,understanding heat-stress biology,and breeding heat-tolerant potato cultivars,especially for sustainable potato production under climate change.
