According to folk usage of Aconitum carmichaelii Debx., the present study was designed to determine the feasibility of the stems and leaves of Aconitum carmichaelii Debx. as a new medicinal resource. Fourteen alkaloid...According to folk usage of Aconitum carmichaelii Debx., the present study was designed to determine the feasibility of the stems and leaves of Aconitum carmichaelii Debx. as a new medicinal resource. Fourteen alkaloids in mother roots, fibrous roots, stems, and leaves of Aconitum carmichaelii Debx. were measured by HPLC-MS/MS. And multivariate analysis methods, such as clustering analysis and principal component analysis, were applied to analyze the difference among various parts. In addition, the acute toxicity, analgesia, and anti-inflammatory tests were carried out. The results suggested that the contents of alkaloids in mother roots and fibrous roots were approximate, but those of leaves and stems were different from mother roots and fibrous roots. The results of the acute toxicity testing demonstrated the toxicity of fibrous root was strongest, and mother roots were slightly less toxic than fibrous roots. The stems and leaves were far less toxic than mother and fibrous roots. In addition, the analgesia and inflammatory tests showed the effects of the various tissues had no difference each other. These results provided a basis for developing new complementary and alternative treatments for rheumatoid arthritis patients. Simultaneously, the approach may also turn wastes into treasure and promote the development of circular economy.展开更多
目的:通过网络药理学和实验验证方法探讨虎杖-吴茱萸配伍抗痛风(gouty arthritis,GA)的作用机制。方法:运用中药系统药理学数据库(traditional chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选出虎...目的:通过网络药理学和实验验证方法探讨虎杖-吴茱萸配伍抗痛风(gouty arthritis,GA)的作用机制。方法:运用中药系统药理学数据库(traditional chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选出虎杖和吴茱萸的活性成分及相关靶点蛋白,采用Cytoscape v3.7.2软件构建活性成分-靶点网络图,借助STRING数据库构建蛋白相互作用网络(protein-protein interaction network,PPI),再利用DAVID数据库进行基因本体(gene ontology,GO)功能与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,预测虎杖-吴茱萸配伍抗痛风的作用机制,进而建立大鼠急性GA模型,采用比色法测定大鼠血清中尿酸(uric acid,UA)与黄嘌呤氧化酶(xanthine oxidase,XOD)水平,并采用ELISA试剂盒检测大鼠血清中白介素-6(interleukin 6,IL-6)、白介素-1β(interleukin 1β,IL-1β)、白介素-10(interleukin 10,IL-10)及白介素-17β(interleukin 17β,IL-17β)指标的含量变化。结果:从虎杖-吴茱萸中共筛选得到26种有效成分,与痛风相关的靶点有21个,其中炎症因子发挥重要作用。验证实验也表明,虎杖-吴茱萸各剂量组能显著降低血清中UA和XOD水平,能显著降低IL-6、IL-1β、IL-17β的含量并增加IL-10的水平。结论:虎杖-吴茱萸的活性成分可调控GA发病重要通路中的多个靶点,初步实验验证其抗GA的作用机制可能是通过炎症相关通路,调控炎症因子发挥作用,为其临床应用提供参考。展开更多
基金supported by the Sichuan Provincial Administration of Traditional Chinese Medicine Research Project(No.2018NQ008)
文摘According to folk usage of Aconitum carmichaelii Debx., the present study was designed to determine the feasibility of the stems and leaves of Aconitum carmichaelii Debx. as a new medicinal resource. Fourteen alkaloids in mother roots, fibrous roots, stems, and leaves of Aconitum carmichaelii Debx. were measured by HPLC-MS/MS. And multivariate analysis methods, such as clustering analysis and principal component analysis, were applied to analyze the difference among various parts. In addition, the acute toxicity, analgesia, and anti-inflammatory tests were carried out. The results suggested that the contents of alkaloids in mother roots and fibrous roots were approximate, but those of leaves and stems were different from mother roots and fibrous roots. The results of the acute toxicity testing demonstrated the toxicity of fibrous root was strongest, and mother roots were slightly less toxic than fibrous roots. The stems and leaves were far less toxic than mother and fibrous roots. In addition, the analgesia and inflammatory tests showed the effects of the various tissues had no difference each other. These results provided a basis for developing new complementary and alternative treatments for rheumatoid arthritis patients. Simultaneously, the approach may also turn wastes into treasure and promote the development of circular economy.
文摘目的:通过网络药理学和实验验证方法探讨虎杖-吴茱萸配伍抗痛风(gouty arthritis,GA)的作用机制。方法:运用中药系统药理学数据库(traditional chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选出虎杖和吴茱萸的活性成分及相关靶点蛋白,采用Cytoscape v3.7.2软件构建活性成分-靶点网络图,借助STRING数据库构建蛋白相互作用网络(protein-protein interaction network,PPI),再利用DAVID数据库进行基因本体(gene ontology,GO)功能与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,预测虎杖-吴茱萸配伍抗痛风的作用机制,进而建立大鼠急性GA模型,采用比色法测定大鼠血清中尿酸(uric acid,UA)与黄嘌呤氧化酶(xanthine oxidase,XOD)水平,并采用ELISA试剂盒检测大鼠血清中白介素-6(interleukin 6,IL-6)、白介素-1β(interleukin 1β,IL-1β)、白介素-10(interleukin 10,IL-10)及白介素-17β(interleukin 17β,IL-17β)指标的含量变化。结果:从虎杖-吴茱萸中共筛选得到26种有效成分,与痛风相关的靶点有21个,其中炎症因子发挥重要作用。验证实验也表明,虎杖-吴茱萸各剂量组能显著降低血清中UA和XOD水平,能显著降低IL-6、IL-1β、IL-17β的含量并增加IL-10的水平。结论:虎杖-吴茱萸的活性成分可调控GA发病重要通路中的多个靶点,初步实验验证其抗GA的作用机制可能是通过炎症相关通路,调控炎症因子发挥作用,为其临床应用提供参考。
