OBJECTIVE This study was aimed to evaluate the effect of mangosteen peel extract(MPE)on migration of human umbilical vein endothelial cells(HUVECs)treated with LPS.METHODS Cultured HUVECs were divided into five groups...OBJECTIVE This study was aimed to evaluate the effect of mangosteen peel extract(MPE)on migration of human umbilical vein endothelial cells(HUVECs)treated with LPS.METHODS Cultured HUVECs were divided into five groups:normal(untreated)group,positive control group treated with LPS at dose of 20ng·mL-1 for 24 h,and groups pre-treated with LPS at dose of 20ng·mL-1 for 24 h followed by incubation with MPE at doses of 1,2,and 4-1μg·mL,respectively.HUVECs migration was evaluated using wound-healing migration assay after 24 h of treatment.RESULTS LPS significantly(P<0.05)impaired HUVECs migration compared to the normal.MPE at dose of 1μg·mL-1 increased migration of LPS-treated HUVECs significantly(P<0.05)compared to the positive control and near to the normal level.CONCLUSION The mangosteen peel extract is able to dose-dependently preserve the endothelial cells function.展开更多
文摘OBJECTIVE This study was aimed to evaluate the effect of mangosteen peel extract(MPE)on migration of human umbilical vein endothelial cells(HUVECs)treated with LPS.METHODS Cultured HUVECs were divided into five groups:normal(untreated)group,positive control group treated with LPS at dose of 20ng·mL-1 for 24 h,and groups pre-treated with LPS at dose of 20ng·mL-1 for 24 h followed by incubation with MPE at doses of 1,2,and 4-1μg·mL,respectively.HUVECs migration was evaluated using wound-healing migration assay after 24 h of treatment.RESULTS LPS significantly(P<0.05)impaired HUVECs migration compared to the normal.MPE at dose of 1μg·mL-1 increased migration of LPS-treated HUVECs significantly(P<0.05)compared to the positive control and near to the normal level.CONCLUSION The mangosteen peel extract is able to dose-dependently preserve the endothelial cells function.
