Chemical exposure can indirectly affect leaf microbiota communities,but the mechanism driving this phenomenon remains largely unknown.Results revealed that the co-exposure of glyphosate and multi-carbon nanotubes(CNTs...Chemical exposure can indirectly affect leaf microbiota communities,but the mechanism driving this phenomenon remains largely unknown.Results revealed that the co-exposure of glyphosate and multi-carbon nanotubes(CNTs)caused a synergistic inhibitory effect on the growth and metabolism of Arabidopsis thaliana shoots.However,only a slight inhibitory effect was induced by nanotubes or glyphosate alone at the tested concentrations.Several intermediate metabolites of nitrogen metabolism and fatty acid synthesis pathways were upregulated under the combined treatment,which increased the amount of energy required to alleviate the disruption caused by the combined treatment.Additionally,compared with the two individual treatments,the glyphosate/nanotube combination treatment induced greater fluctuations in the phyllosphere bacterial community members with low abundance(relative abundance(RA)<1%)at both the family and genus levels,and among these bacteria some plant growth promotion and nutrient supplement related bacteria were markable increased.Strikingly,strong correlations between phyllosphere bacterial diversity and metabolites suggested a potential role of leaf metabolism,particularly nitrogen and carbohydrate metabolism,in restricting the range of leaf microbial taxa.These correlations between phyllosphere bacterial diversity and leaf metabolism will improve our understanding of plant-microbe interactions and the extent of their drivers of variation and the underlying causes of variability in bacterial community composition.展开更多
The soil nitrogen cycle is primarily driven by microbial communities and provides reactive nitrogen for all organisms.With the increasing impact of human activities and climate change,biogeographical explicit patterns...The soil nitrogen cycle is primarily driven by microbial communities and provides reactive nitrogen for all organisms.With the increasing impact of human activities and climate change,biogeographical explicit patterns of soil microbial nitrogen-cycling genes and their associations with nitrogen fluxes are still unknown at the global scale.By conducting a global analysis of 1198 soil metagenomic samples,we verified that agricultural land displayed lower microbial richness and diversity values than did the other habitats.We generated a global map of the genetic potential of N cycle processes in soil and revealed that denitrification and dissimilatory nitrate reduction processes are greater in agricultural centers than in non-agricultural areas and are mainly driven by the mean annual temperature and nitrogen fertilizer application.Soil nitrous oxide(N2O)emissions are greater in agricultural land than in other habitats and are mainly driven by nitrogen fertilizer application,which is consistent with the genetic potential of N2O synthesis.Our study improves the theoretical framework for predicting global soil nitrogen cycling potential under complex variables and highlights the influence weight of human activities and climate factors.We strongly emphasize the importance of rationally applying nitrogen fertilizers to balance agricultural production,ecological health and climate change.展开更多
●6102 high-quality sequencing results of soil bacterial samples were re-analyzed.●The type of land use was the principal driver of bacterial richness and diversity.●SOC content is positively correlated with key bac...●6102 high-quality sequencing results of soil bacterial samples were re-analyzed.●The type of land use was the principal driver of bacterial richness and diversity.●SOC content is positively correlated with key bacteria and total nitrogen content.Soil organic carbon(SOC)is the largest pool of carbon in terrestrial ecosystems and plays a crucial role in regulating atmospheric CO_(2) concentrations.Identifying the essential relationship between soil bacterial communities and SOC concentration is complicated because of many factors,one of which is geography.We systematically re-analyzed 6102 high-quality bacterial samples in China to delineate the bacterial biogeographic distribution of bacterial communities and identify key species associated with SOC concentration at the continental scale.The type of land use was the principal driver of bacterial richness and diversity,and we used machine learning to calculate its influence on microbial composition and their co-occurrence relationship with SOC concentration.Cultivated land was much more complex than forest,grassland,wetland and wasteland,with high SOC concentrations tending to enrich bacteria such as Rubrobacter,Terrimonas and Sphingomona.SOC concentration was positively correlated with the amounts of soil total nitrogen and key bacteria Xanthobacteraceae,Streptomyces and Acidobacteria but was negatively correlated with soil pH,total phosphorus and Micrococcaceae.Our study combined the SOC pool with bacteria and indicated that specific bacteria may be key factors affecting SOC concentration,forcing us to think about microbial communities associated with climate change in a new way.展开更多
Chlorine-containing disinfectants have been widely used around the world for the prevention and control of the COVID-19 pandemic.However,at present,little is known about the impact of residual chlorine on the soil mic...Chlorine-containing disinfectants have been widely used around the world for the prevention and control of the COVID-19 pandemic.However,at present,little is known about the impact of residual chlorine on the soil micro-ecological environment.Herein,we treated an experimental soil-plant-microbiome microcosm system by continuous irrigation with a low concentration of chlorine-containing water,and then analyzed the influence on the soil microbial community using metagenomics.After 14-d continuous chlorine treatment,there were no significant lasting effect on soil microbial community diversity and composition either in the rhizosphere or in bulk soil.Although metabolic functions of the rhizosphere microbial community were affected slightly by continuous chlorine treatment,it recovered to the original status.The abundance of several resistance genes changed by 7 d and recovered by 14 d.According to our results,the chlorine residue resulting from daily disinfection may present a slight long-term effect on plant growth(shoot length and fresh weight)and soil micro-ecology.In general,our study assisted with environmental risk assessments relating to the application of chlorine-containing disinfectants and minimization of risks to the environment during disease control,such as COVID-19.展开更多
基金supported by the National Natural Science Foundation of China(Nos.21777144,21976161,41907210)the Changjiang Scholars and Innovative Research Team in University(No.IRT_17R97)。
文摘Chemical exposure can indirectly affect leaf microbiota communities,but the mechanism driving this phenomenon remains largely unknown.Results revealed that the co-exposure of glyphosate and multi-carbon nanotubes(CNTs)caused a synergistic inhibitory effect on the growth and metabolism of Arabidopsis thaliana shoots.However,only a slight inhibitory effect was induced by nanotubes or glyphosate alone at the tested concentrations.Several intermediate metabolites of nitrogen metabolism and fatty acid synthesis pathways were upregulated under the combined treatment,which increased the amount of energy required to alleviate the disruption caused by the combined treatment.Additionally,compared with the two individual treatments,the glyphosate/nanotube combination treatment induced greater fluctuations in the phyllosphere bacterial community members with low abundance(relative abundance(RA)<1%)at both the family and genus levels,and among these bacteria some plant growth promotion and nutrient supplement related bacteria were markable increased.Strikingly,strong correlations between phyllosphere bacterial diversity and metabolites suggested a potential role of leaf metabolism,particularly nitrogen and carbohydrate metabolism,in restricting the range of leaf microbial taxa.These correlations between phyllosphere bacterial diversity and leaf metabolism will improve our understanding of plant-microbe interactions and the extent of their drivers of variation and the underlying causes of variability in bacterial community composition.
基金supported by the National Natural Science Foundation of China(Grant Nos.42377107,22376187 and 42307158).
文摘The soil nitrogen cycle is primarily driven by microbial communities and provides reactive nitrogen for all organisms.With the increasing impact of human activities and climate change,biogeographical explicit patterns of soil microbial nitrogen-cycling genes and their associations with nitrogen fluxes are still unknown at the global scale.By conducting a global analysis of 1198 soil metagenomic samples,we verified that agricultural land displayed lower microbial richness and diversity values than did the other habitats.We generated a global map of the genetic potential of N cycle processes in soil and revealed that denitrification and dissimilatory nitrate reduction processes are greater in agricultural centers than in non-agricultural areas and are mainly driven by the mean annual temperature and nitrogen fertilizer application.Soil nitrous oxide(N2O)emissions are greater in agricultural land than in other habitats and are mainly driven by nitrogen fertilizer application,which is consistent with the genetic potential of N2O synthesis.Our study improves the theoretical framework for predicting global soil nitrogen cycling potential under complex variables and highlights the influence weight of human activities and climate factors.We strongly emphasize the importance of rationally applying nitrogen fertilizers to balance agricultural production,ecological health and climate change.
基金We appreciate Liu et al.for their latest data(Liu et al.,2020a,2020b,2022)on national SOC,pH,as well as the contents of total nitrogen,phosphorus and potassium.Funding was provided by the Key R&D Program of Zhejiang Province(2022C02046 and 2022C02029)the National Natural Science Foundation of China(21976161 and 21777145)J.P.acknowledges funding from the Spanish Government grant PID2019-110521GB-I00,the Fundación Ramón Areces grant CIVP20A6621,and the Catalan Government grant SGR2017-1005.
文摘●6102 high-quality sequencing results of soil bacterial samples were re-analyzed.●The type of land use was the principal driver of bacterial richness and diversity.●SOC content is positively correlated with key bacteria and total nitrogen content.Soil organic carbon(SOC)is the largest pool of carbon in terrestrial ecosystems and plays a crucial role in regulating atmospheric CO_(2) concentrations.Identifying the essential relationship between soil bacterial communities and SOC concentration is complicated because of many factors,one of which is geography.We systematically re-analyzed 6102 high-quality bacterial samples in China to delineate the bacterial biogeographic distribution of bacterial communities and identify key species associated with SOC concentration at the continental scale.The type of land use was the principal driver of bacterial richness and diversity,and we used machine learning to calculate its influence on microbial composition and their co-occurrence relationship with SOC concentration.Cultivated land was much more complex than forest,grassland,wetland and wasteland,with high SOC concentrations tending to enrich bacteria such as Rubrobacter,Terrimonas and Sphingomona.SOC concentration was positively correlated with the amounts of soil total nitrogen and key bacteria Xanthobacteraceae,Streptomyces and Acidobacteria but was negatively correlated with soil pH,total phosphorus and Micrococcaceae.Our study combined the SOC pool with bacteria and indicated that specific bacteria may be key factors affecting SOC concentration,forcing us to think about microbial communities associated with climate change in a new way.
基金financially supported by the National Natural Science Foundation of China(41907210,71903079,21976161,21777144).
文摘Chlorine-containing disinfectants have been widely used around the world for the prevention and control of the COVID-19 pandemic.However,at present,little is known about the impact of residual chlorine on the soil micro-ecological environment.Herein,we treated an experimental soil-plant-microbiome microcosm system by continuous irrigation with a low concentration of chlorine-containing water,and then analyzed the influence on the soil microbial community using metagenomics.After 14-d continuous chlorine treatment,there were no significant lasting effect on soil microbial community diversity and composition either in the rhizosphere or in bulk soil.Although metabolic functions of the rhizosphere microbial community were affected slightly by continuous chlorine treatment,it recovered to the original status.The abundance of several resistance genes changed by 7 d and recovered by 14 d.According to our results,the chlorine residue resulting from daily disinfection may present a slight long-term effect on plant growth(shoot length and fresh weight)and soil micro-ecology.In general,our study assisted with environmental risk assessments relating to the application of chlorine-containing disinfectants and minimization of risks to the environment during disease control,such as COVID-19.
