Recent advancements in thermal conductivity modulating strategies have shown promising enhancements to the thermal management capabilities of two-dimensional materials.In this article,both the iterative Boltzmann tran...Recent advancements in thermal conductivity modulating strategies have shown promising enhancements to the thermal management capabilities of two-dimensional materials.In this article,both the iterative Boltzmann transport equation solution and the two-temperature model were employed to investigate the efficacy of targeted phonon excitation applied to hexagonal boron nitride(hBN).The results indicate significant modifications to hBN's thermal conductivity,achieving increases of up to 30.1%as well as decreases of up to 59.8%.These findings validate the reliability of the strategy,expand its scope of applicability,and establish it as a powerful tool for tailoring thermal properties across a wider range of fields.展开更多
Aptamers are a class of single oligonucleotide molecules(DNA or RNA)that are screened from random DNA or RNA oligonucleotide chain libraries by the systemic evolution of ligands by exponential enrichment technology.Th...Aptamers are a class of single oligonucleotide molecules(DNA or RNA)that are screened from random DNA or RNA oligonucleotide chain libraries by the systemic evolution of ligands by exponential enrichment technology.The selected aptamers are capable of specifically binding to different targeting molecules,which is achieved by the three-dimensional structure of aptamers.Aptamers are similar in function to monoclonal antibodies,and therefore,they are also referred to as"chemical antibodies".Due to their high affinity and specificity and low immunogenicity,aptamers are topics of intense interest in today's biological targeting research especially in tumor research.They not only have high potential for clinical advances in tumor targeting detection but also are highly promising as targeted tumor drug carriers for use in tumor therapy.Various experimental studies have shown that aptamer-based diagnostic and therapeutic methods for liver cancer have great potential for application.This paper summarizes the structure,characteristics,and screening methods of aptamers and reviews the recent research progress on nucleic acid aptamers in the targeted diagnosis and treatment of liver cancer.展开更多
We study the mechanism of van der Waals(vdW)interactions on phonon transport in atomic scale,which would boost developments in heat management and energy conversion.Commonly,the vdW interactions are regarded as a hind...We study the mechanism of van der Waals(vdW)interactions on phonon transport in atomic scale,which would boost developments in heat management and energy conversion.Commonly,the vdW interactions are regarded as a hindrance in phonon transport.Here we propose that the vdW confinement can enhance phonon transport.Through molecular dynamics simulations,it is realized that the vdW confinement is able to make more than two-fold enhancement on thermal conductivity of both polyethylene single chain and graphene nanoribbon.The quantitative analyses of morphology,local vdW potential energy and dynamical properties are carried out to reveal the underlying physical mechanism.It is found that the confined vdW potential barriers reduce the atomic thermal displacement magnitudes,leading to less phonon scattering and facilitating thermal transport.Our study offers a new strategy to modulate the phonon transport.展开更多
Objective:To investigate the effects of preoperative portal venous injection of donor spleen cells(PVIDSC) and intraperitoneal injection of rapamycin in the acute rejection of cardiac allograft in mice and the underly...Objective:To investigate the effects of preoperative portal venous injection of donor spleen cells(PVIDSC) and intraperitoneal injection of rapamycin in the acute rejection of cardiac allograft in mice and the underlying mechanisms. Methods:Homogenous female B6 mice and BALB/c mice were used as recipients and donors of heart transplantation. These mice were randomly divided into different groups and received PVIDSC alone,rapamycin alone,or PVIDSC and rapamycin combined therapy. In addition,the underlying mechanism was studied by measuring a number of cytokines. Results:Preoperative combination of PVIDSC and intraperitoneal injection of rapamycin significantly prolonged the survival of heterotopic cardiac allograft in mice,but had no effects on the survival time of cardiac allografts in mice pre-sensitized by skin grafting. Preoperative combination of PVIDSC and intraperitoneal injection of rapamycin increased the expression of IL-10 and Foxp3 and reduced the expression of INF-γ. Short-term preoperative administration of rapamycin promotes the expression of CD4^+CD25^+Foxp3^+ regulator T cells. However,preoperative using alone of rapamycin,or combination of PVIDSC and rapamycin had no effects on the inhibition of proliferation of memory T cells. Conclusions:Preoperative application of combination of PVIDSC and rapamycin significantly prolonged the survival time of cardiac allografts in mice but not in mice pre-sensitized by skin grafting. This may be explained by the fact that combination of PVIDSC and rapamycin inhibited the cellular immune response and induced the expression of IL-10 from Tr1 cells and CD4^+CD25^+FoxP3^+ regulatory T cells.展开更多
Exploring the mechanism of interfacial thermal transport and reducing the interfacial thermal resistance are of great importance for thermal management and modulation.Herein,the interfacial thermal resistance between ...Exploring the mechanism of interfacial thermal transport and reducing the interfacial thermal resistance are of great importance for thermal management and modulation.Herein,the interfacial thermal resistance between overlapped graphene nanoribbons is largely reduced by adding bonded carbon chains as shown by molecular dynamics simulations.And the analytical model(phonon weak couplings model,PWCM)is utilized to analyze and explain the two-dimensional thermal transport mechanism at the cross-interface.An order of magnitude reduction of the interfacial thermal resistance is found as the graphene nanoribbons are bonded by just one carbon chain.Interestingly,the decreasing rate of the interfacial thermal resistance slows down gradually with the increasing number of carbon chains,which can be explained by the proposed theoretical relationship based on analytical model.Moreover,by the comparison of PWCM and the traditional simplified model,the accuracy of PWCM is demonstrated in the overlapped graphene nanoribbons.This work provides a new way to improve the interfacial thermal transport and reveal the essential mechanism for low-dimensional materials applied in thermal management.展开更多
With the rapid increase in power density of electronic devices,thermal management has become urgent for the electronics industry.Controlling temperature in the back-end-of-line is crucial for maintaining the reliabili...With the rapid increase in power density of electronic devices,thermal management has become urgent for the electronics industry.Controlling temperature in the back-end-of-line is crucial for maintaining the reliability of integrated circuits,where many atomic-scale interfaces exist.The theoretical models of interface thermal conductance not only accurately predict the values but also help to analyze the underlying mechanism.This review picks up and introduces some representative theoretical models considering interfacial roughness,elastic and inelastic processes,and electron–phonon couplings,etc.Moreover,the limitations and problems of these models are also discussed.展开更多
Leidenfrost effect is a common and important phenomenon which has many applications,however there is a limited body of knowledge about the Leidenfrost effect at the nanoscale regime.We investigate the impact of substr...Leidenfrost effect is a common and important phenomenon which has many applications,however there is a limited body of knowledge about the Leidenfrost effect at the nanoscale regime.We investigate the impact of substrate wettability on Leidenfrost point temperature(LPT) of nanoscale water film via molecular dynamics simulations,and reveal a new mechanism different from that at the macroscale.In the molecular dynamics simulations,a method of monitoring density change at different heating rates is proposed to obtain accurate LPT under different surface wettability.The results show that LPT decreases firstly and then increases with the surface wettability at the nanoscale,which is different from the monotonous increasing trend at the macroscale.The mechanism is elucidated by analyzing the competitive effect of adhesion force and interfacial thermal resistance,as well as different contributions of gravity on LPT at the nanoscale and macroscale.The investigations can deepen the understanding of Leidenfrost effect at the nanoscale regime and also facilitate to guide the applications of heat transfer and flow transport.展开更多
Transcranial alternating current stimulation(tACS)is a noninvasive neuromodulation technique that regulates brain function by applying sinusoidal alternating currents of specific frequencies to targeted brain regions ...Transcranial alternating current stimulation(tACS)is a noninvasive neuromodulation technique that regulates brain function by applying sinusoidal alternating currents of specific frequencies to targeted brain regions through the scalp electrodes.[1]This guideline aims to evaluate and summarize the efficacy and safety of tACS in clinical applications.We conducted a structured literature search across major databases using the terms“tACS”or“transcranial alternating current stimulation”to identify all relevant literature up to June 2022.展开更多
Recently, emerging phonon phenomena have been discovered and rapidly developed, which have become an active hot research topic. In this review article, we present state-of-the-art advances in several fascinating phono...Recently, emerging phonon phenomena have been discovered and rapidly developed, which have become an active hot research topic. In this review article, we present state-of-the-art advances in several fascinating phonon transport phenomena. First, we summarize the recent progress on the wave nature of phonons, including phonon coherence and its effects on thermal conductivity and the topological properties of phonons. Then, we discuss the particle nature of phonons, including the weak coupling of phonons and the high-order phonon anharmonicity. Finally, we present the summary and a brief outlook. This review presents the advanced understanding of some emerging phonon phenomena in solid materials, which provides new opportunities for further advancement in a wide variety of applications.展开更多
Non-human primates(NHPs)represent the most valuable animals for drug discovery.However,the current main challenge remains that the NHP has not yet been used to develop an efficient translational medicine platform simu...Non-human primates(NHPs)represent the most valuable animals for drug discovery.However,the current main challenge remains that the NHP has not yet been used to develop an efficient translational medicine platform simulating human diseases,such as cancer.This study gen erated an in situ gen e-editi ng approach to in duce efficie nt loss-of-function mutations of Pten and p53 genes for rapid modeling primary and metastatic liver tumors using the CRISPR/Cas9 in the adult cyno molgus mon key.Un der ultraso und guida nee,the CRISPR/Cas9 was injected into the cyno molgus mon key liver through the in trahepatic portal vein.The results showed that the ultraso un d-guided CRISPR/Cas9 resulted in in dels of the Pten and p53 genes in seve n out of eight monkeys.The best mutation efficiencies for Pten and p53 were up to 74.71%and 74.68%,respectively.Furthermore,the morbidity of primary and exte nsively metastatic(lung,splee n,lymph nodes)hepatoma in CRISPR-treated mon keys was 87.5%.The ultraso un d-guided CRISPR system could have great potential to successfully pursue the desired target genes,thereby reducing possible side effects associated with hitting no n-specific off-target genes,and sign ifica ntly in creasing more efficie ncy as well as higher specificity of in situ gene editi ng in vivo,which holds promise as a powerful,yet feasible tool,to edit disease genes to build corresp on ding huma n disease models in adult NHPs and to greatly accelerate the discovery of new drugs and save economic costs.展开更多
Introduction:The rapid fluorescent focus inhibition test(RFFIT)is a cell-based virus neutralization assay and the gold standard for quantifying rabies virus neutralizing antibodies(RVNA)in serums.It is used to assess ...Introduction:The rapid fluorescent focus inhibition test(RFFIT)is a cell-based virus neutralization assay and the gold standard for quantifying rabies virus neutralizing antibodies(RVNA)in serums.It is used to assess the biological efficacy of rabies vaccines and evaluate protective immunity in both humans and animals.Despite its broad application,RFFIT requires thorough validation to ensure reliability.Methods:RFFIT was validated in this study using the third World Health Organization international standard for anti-rabies immunoglobulin(WHO-3 SRIG)and negative human sera.The validation followed the guidelines outlined by the Food and Drug Administration Guidance for Industry and International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use(ICH)Q2(R1)guidelines and included the assessment of intra-assay and intermediate precision,dilutability,linearity,range,accuracy,specificity,robustness,and stability.Results:The RFFIT method demonstrated good precision,with intra-assay and intermediate-precision geometric coefficient of variation(GCV)<30%.Dilutability was confirmed,with 95%of positive samples showing geometric mean concentration(GMC)differences within±30%compared to undiluted controls.The standard and detection values were described by y=1.0091x−0.1128(R^(2)=0.9948);95.56%of the samples showed 70%–130%recovery.Specificity was verified using homologous and heterologous antigen competition and a matrix with no significant cross-reactivity.The assay was robust to variations in cells,reagents,and time,with titer differences within±30%.Stability of samples and reagents under freeze–thaw and different short-term storage conditions was confirmed.Conclusion:The assay was successfully validated for quantifying RVNA content in serum samples.展开更多
基金supported by the National Key Research and Development Project of China(Grant No.2018YFE0127800)。
文摘Recent advancements in thermal conductivity modulating strategies have shown promising enhancements to the thermal management capabilities of two-dimensional materials.In this article,both the iterative Boltzmann transport equation solution and the two-temperature model were employed to investigate the efficacy of targeted phonon excitation applied to hexagonal boron nitride(hBN).The results indicate significant modifications to hBN's thermal conductivity,achieving increases of up to 30.1%as well as decreases of up to 59.8%.These findings validate the reliability of the strategy,expand its scope of applicability,and establish it as a powerful tool for tailoring thermal properties across a wider range of fields.
文摘Aptamers are a class of single oligonucleotide molecules(DNA or RNA)that are screened from random DNA or RNA oligonucleotide chain libraries by the systemic evolution of ligands by exponential enrichment technology.The selected aptamers are capable of specifically binding to different targeting molecules,which is achieved by the three-dimensional structure of aptamers.Aptamers are similar in function to monoclonal antibodies,and therefore,they are also referred to as"chemical antibodies".Due to their high affinity and specificity and low immunogenicity,aptamers are topics of intense interest in today's biological targeting research especially in tumor research.They not only have high potential for clinical advances in tumor targeting detection but also are highly promising as targeted tumor drug carriers for use in tumor therapy.Various experimental studies have shown that aptamer-based diagnostic and therapeutic methods for liver cancer have great potential for application.This paper summarizes the structure,characteristics,and screening methods of aptamers and reviews the recent research progress on nucleic acid aptamers in the targeted diagnosis and treatment of liver cancer.
基金Supported by the National Natural Science Foundation of China(Grant Nos.51606072 and 51576077).
文摘We study the mechanism of van der Waals(vdW)interactions on phonon transport in atomic scale,which would boost developments in heat management and energy conversion.Commonly,the vdW interactions are regarded as a hindrance in phonon transport.Here we propose that the vdW confinement can enhance phonon transport.Through molecular dynamics simulations,it is realized that the vdW confinement is able to make more than two-fold enhancement on thermal conductivity of both polyethylene single chain and graphene nanoribbon.The quantitative analyses of morphology,local vdW potential energy and dynamical properties are carried out to reveal the underlying physical mechanism.It is found that the confined vdW potential barriers reduce the atomic thermal displacement magnitudes,leading to less phonon scattering and facilitating thermal transport.Our study offers a new strategy to modulate the phonon transport.
基金supported,in part,by grants from National Science and Technology Major Project---national major new drug creation (No. 2015GKS-462)National Natural Science Foundation of key projects (No. 81430055)+2 种基金National "Chang Jiang Scholars and Innovative Team Development Program" Innovation Team Rolling Support Project (No. IRT_15R13)Guangxi Science Research and Technology Development Project (No.Gui Ke He 1599005-2-10)Inter-provincial cooperation projects (No.Gui Ke He 14251001)
文摘Objective:To investigate the effects of preoperative portal venous injection of donor spleen cells(PVIDSC) and intraperitoneal injection of rapamycin in the acute rejection of cardiac allograft in mice and the underlying mechanisms. Methods:Homogenous female B6 mice and BALB/c mice were used as recipients and donors of heart transplantation. These mice were randomly divided into different groups and received PVIDSC alone,rapamycin alone,or PVIDSC and rapamycin combined therapy. In addition,the underlying mechanism was studied by measuring a number of cytokines. Results:Preoperative combination of PVIDSC and intraperitoneal injection of rapamycin significantly prolonged the survival of heterotopic cardiac allograft in mice,but had no effects on the survival time of cardiac allografts in mice pre-sensitized by skin grafting. Preoperative combination of PVIDSC and intraperitoneal injection of rapamycin increased the expression of IL-10 and Foxp3 and reduced the expression of INF-γ. Short-term preoperative administration of rapamycin promotes the expression of CD4^+CD25^+Foxp3^+ regulator T cells. However,preoperative using alone of rapamycin,or combination of PVIDSC and rapamycin had no effects on the inhibition of proliferation of memory T cells. Conclusions:Preoperative application of combination of PVIDSC and rapamycin significantly prolonged the survival time of cardiac allografts in mice but not in mice pre-sensitized by skin grafting. This may be explained by the fact that combination of PVIDSC and rapamycin inhibited the cellular immune response and induced the expression of IL-10 from Tr1 cells and CD4^+CD25^+FoxP3^+ regulatory T cells.
基金Project supported by the National Natural Science Foundation of China(Grant No.51606072)the Fundamental Research Funds for the Central Universities,HUST,China(Grant No.2019kfyRCPY045)。
文摘Exploring the mechanism of interfacial thermal transport and reducing the interfacial thermal resistance are of great importance for thermal management and modulation.Herein,the interfacial thermal resistance between overlapped graphene nanoribbons is largely reduced by adding bonded carbon chains as shown by molecular dynamics simulations.And the analytical model(phonon weak couplings model,PWCM)is utilized to analyze and explain the two-dimensional thermal transport mechanism at the cross-interface.An order of magnitude reduction of the interfacial thermal resistance is found as the graphene nanoribbons are bonded by just one carbon chain.Interestingly,the decreasing rate of the interfacial thermal resistance slows down gradually with the increasing number of carbon chains,which can be explained by the proposed theoretical relationship based on analytical model.Moreover,by the comparison of PWCM and the traditional simplified model,the accuracy of PWCM is demonstrated in the overlapped graphene nanoribbons.This work provides a new way to improve the interfacial thermal transport and reveal the essential mechanism for low-dimensional materials applied in thermal management.
基金supported by the Department of Packaging and Testing Institution of Sanechips。
文摘With the rapid increase in power density of electronic devices,thermal management has become urgent for the electronics industry.Controlling temperature in the back-end-of-line is crucial for maintaining the reliability of integrated circuits,where many atomic-scale interfaces exist.The theoretical models of interface thermal conductance not only accurately predict the values but also help to analyze the underlying mechanism.This review picks up and introduces some representative theoretical models considering interfacial roughness,elastic and inelastic processes,and electron–phonon couplings,etc.Moreover,the limitations and problems of these models are also discussed.
基金Supported by the National Key Research and Development Program of China(Grant No.2018YFE0127800)。
文摘Leidenfrost effect is a common and important phenomenon which has many applications,however there is a limited body of knowledge about the Leidenfrost effect at the nanoscale regime.We investigate the impact of substrate wettability on Leidenfrost point temperature(LPT) of nanoscale water film via molecular dynamics simulations,and reveal a new mechanism different from that at the macroscale.In the molecular dynamics simulations,a method of monitoring density change at different heating rates is proposed to obtain accurate LPT under different surface wettability.The results show that LPT decreases firstly and then increases with the surface wettability at the nanoscale,which is different from the monotonous increasing trend at the macroscale.The mechanism is elucidated by analyzing the competitive effect of adhesion force and interfacial thermal resistance,as well as different contributions of gravity on LPT at the nanoscale and macroscale.The investigations can deepen the understanding of Leidenfrost effect at the nanoscale regime and also facilitate to guide the applications of heat transfer and flow transport.
基金supported by grants from the National Key Research and Development Program of China(Nos.2021YFC2501400,2021YFC2501404).
文摘Transcranial alternating current stimulation(tACS)is a noninvasive neuromodulation technique that regulates brain function by applying sinusoidal alternating currents of specific frequencies to targeted brain regions through the scalp electrodes.[1]This guideline aims to evaluate and summarize the efficacy and safety of tACS in clinical applications.We conducted a structured literature search across major databases using the terms“tACS”or“transcranial alternating current stimulation”to identify all relevant literature up to June 2022.
基金supported in part by the National Natural Science Foundation of China (Grant Nos. 11890703, and 12075168)the Science and Technology Commission of Shanghai Municipality (Grant Nos. 19ZR1478600, and 21JC1405600)+3 种基金the Fundamental Research Funds for the Central Universities (Grant No. 22120220060)supported in part by the RIE2020 Advanced Manufacturing and Engineering (AME) Programmatic (Grant No. A1898b0043)Singapore Aerospace Programme Cycle 15 (Grant No. M2115a0092)supported by the Singapore Ministry of Education AcRF Tier 2 (Grant No. T2EP50220-0026)。
文摘Recently, emerging phonon phenomena have been discovered and rapidly developed, which have become an active hot research topic. In this review article, we present state-of-the-art advances in several fascinating phonon transport phenomena. First, we summarize the recent progress on the wave nature of phonons, including phonon coherence and its effects on thermal conductivity and the topological properties of phonons. Then, we discuss the particle nature of phonons, including the weak coupling of phonons and the high-order phonon anharmonicity. Finally, we present the summary and a brief outlook. This review presents the advanced understanding of some emerging phonon phenomena in solid materials, which provides new opportunities for further advancement in a wide variety of applications.
基金This work was supported in part by Scientific and Technological Innovation Major Base of Guangxi(No.2018-15-Z04)Changjiang Scholars and Innovative Research Team in University(No.IRT_15R13)+2 种基金Guangxi Science and Technology Base and Talent Special Project(No.AD17129062)Guangxi Key Research and Development Project(No.AB20117001)Guangxi Science Foundation Project(No.2018JJA140524).
文摘Non-human primates(NHPs)represent the most valuable animals for drug discovery.However,the current main challenge remains that the NHP has not yet been used to develop an efficient translational medicine platform simulating human diseases,such as cancer.This study gen erated an in situ gen e-editi ng approach to in duce efficie nt loss-of-function mutations of Pten and p53 genes for rapid modeling primary and metastatic liver tumors using the CRISPR/Cas9 in the adult cyno molgus mon key.Un der ultraso und guida nee,the CRISPR/Cas9 was injected into the cyno molgus mon key liver through the in trahepatic portal vein.The results showed that the ultraso un d-guided CRISPR/Cas9 resulted in in dels of the Pten and p53 genes in seve n out of eight monkeys.The best mutation efficiencies for Pten and p53 were up to 74.71%and 74.68%,respectively.Furthermore,the morbidity of primary and exte nsively metastatic(lung,splee n,lymph nodes)hepatoma in CRISPR-treated mon keys was 87.5%.The ultraso un d-guided CRISPR system could have great potential to successfully pursue the desired target genes,thereby reducing possible side effects associated with hitting no n-specific off-target genes,and sign ifica ntly in creasing more efficie ncy as well as higher specificity of in situ gene editi ng in vivo,which holds promise as a powerful,yet feasible tool,to edit disease genes to build corresp on ding huma n disease models in adult NHPs and to greatly accelerate the discovery of new drugs and save economic costs.
文摘Introduction:The rapid fluorescent focus inhibition test(RFFIT)is a cell-based virus neutralization assay and the gold standard for quantifying rabies virus neutralizing antibodies(RVNA)in serums.It is used to assess the biological efficacy of rabies vaccines and evaluate protective immunity in both humans and animals.Despite its broad application,RFFIT requires thorough validation to ensure reliability.Methods:RFFIT was validated in this study using the third World Health Organization international standard for anti-rabies immunoglobulin(WHO-3 SRIG)and negative human sera.The validation followed the guidelines outlined by the Food and Drug Administration Guidance for Industry and International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use(ICH)Q2(R1)guidelines and included the assessment of intra-assay and intermediate precision,dilutability,linearity,range,accuracy,specificity,robustness,and stability.Results:The RFFIT method demonstrated good precision,with intra-assay and intermediate-precision geometric coefficient of variation(GCV)<30%.Dilutability was confirmed,with 95%of positive samples showing geometric mean concentration(GMC)differences within±30%compared to undiluted controls.The standard and detection values were described by y=1.0091x−0.1128(R^(2)=0.9948);95.56%of the samples showed 70%–130%recovery.Specificity was verified using homologous and heterologous antigen competition and a matrix with no significant cross-reactivity.The assay was robust to variations in cells,reagents,and time,with titer differences within±30%.Stability of samples and reagents under freeze–thaw and different short-term storage conditions was confirmed.Conclusion:The assay was successfully validated for quantifying RVNA content in serum samples.
