建立了一种超高效液相色谱串联质谱法(UPLC-MS/MS)同时测定土壤中8种多溴联苯醚化合物(PBDEs)的方法,采用快速溶剂萃取(ASE)和凝胶净化色谱(GPC),经BEH反相C 18色谱柱分离,10 min内完成分离,使用含0.1%氨水的甲醇和水进行梯度洗脱,采用...建立了一种超高效液相色谱串联质谱法(UPLC-MS/MS)同时测定土壤中8种多溴联苯醚化合物(PBDEs)的方法,采用快速溶剂萃取(ASE)和凝胶净化色谱(GPC),经BEH反相C 18色谱柱分离,10 min内完成分离,使用含0.1%氨水的甲醇和水进行梯度洗脱,采用液相色谱-负离子-大气压化学电离(APCI)串联质谱,多反应监测模式(MRM),加入13 C 12-BDE-139和13 C 12-BDE-209内标作定量分析。结果表明:在优化的分析条件下,PBDEs的线性相关系数为0.9988~0.9995,方法检出限为0.22~0.59μg/kg,在2个添加水平下,平均回收率为71%~126%,标准偏差为3%~18%,土壤实际质控样测定结果均在允许范围内。适合于土壤中PBDEs的快速分析,特别对于高溴代PBDEs,相比气相色谱质谱方法具有更快的分析速度和更好的稳定性。展开更多
建立离子色谱法同时测定环境空气和废气中二甲胺、二乙胺和三乙胺等挥发性胺类化合物的方法。以甲磺酸(MSA)-乙腈水溶液为吸收液,采集环境空气或废气中的挥发性胺类化合物,利用Metrosep C4-250/4.0阳离子交换柱分离目标化合物,881 compa...建立离子色谱法同时测定环境空气和废气中二甲胺、二乙胺和三乙胺等挥发性胺类化合物的方法。以甲磺酸(MSA)-乙腈水溶液为吸收液,采集环境空气或废气中的挥发性胺类化合物,利用Metrosep C4-250/4.0阳离子交换柱分离目标化合物,881 compact IC pro 1电导检测器进行检测,以保留时间定性、峰面积定量。结果显示:二甲胺、二乙胺和三乙胺标准曲线线性关系良好,相关系数在0.9992~0.9999之间,废气中二甲胺、二乙胺和三乙胺的检出限分别为0.24,0.30和0.42 mg/m^(3);环境空气中二甲胺、二乙胺和三乙胺的检出限分别为0.06,0.10和0.14 mg/m~3。该方法样品无需复杂的前处理过程,操作简单、分析速度快、准确度高,可同时测定共存于环境空气和废气中的二甲胺、二乙胺和三乙胺。展开更多
Few studies have investigated the properties and protein composition of small extracellular vesicles (sEVs) derived from neurons under hypoxic conditions. Presently, the extent of the involvement of these plentiful sE...Few studies have investigated the properties and protein composition of small extracellular vesicles (sEVs) derived from neurons under hypoxic conditions. Presently, the extent of the involvement of these plentiful sEVs in the onset and progression of ischemic stroke remains an unresolved question. Our study systematically identified the characteristics of sEVs derived from neurons under hypoxic conditions (HypEVs) by physical characterization, sEV absorption, proteomics and transcriptomics analysis. The effects of HypEVs on neurites, cell survival, and neuron structure were assessed in vitro and in vivo by neural complexity tests, magnetic resonance imaging (MRI), Golgi staining, and Western blotting of synaptic plasticity-related proteins and apoptotic proteins. Knockdown of Fused in Sarcoma (FUS) small interfering RNA (siRNA) was used to validate FUS-mediated HypEV neuroprotection and mitochondrial mRNA release. Hypoxia promoted the secretion of sEVs, and HypEVs were more easily taken up and utilized by recipient cells. The MRI results illustrated that the cerebral infarction volume was reduced by 45% with the application of HypEVs, in comparison to the non- HypEV treatment group. Mechanistically, the FUS protein is necessary for the uptake and neuroprotection of HypEVs against ischemic stroke as well as carrying a large amount of mitochondrial mRNA in HypEVs. However, FUS knockdown attenuated the neuroprotective rescue capabilities of HypEVs. Our comprehensive dataset clearly illustrates that FUS-mediated HypEVs deliver exceptional neuroprotective effects against ischemic stroke, primarily through the maintenance of neurite integrity and the reduction of mitochondria-associated apoptosis.展开更多
Experts and patients are calling for a national blood donation system after some patients were forced to wait for treatment due to the fragmented system which does not recognize donations across different regions。
China is getting tough on Big Pharma's monopolistic practices,slapping huge fines on firms that corner the market in crucial medications.Experts question whether fines are enough to deter the shady behavior when d...China is getting tough on Big Pharma's monopolistic practices,slapping huge fines on firms that corner the market in crucial medications.Experts question whether fines are enough to deter the shady behavior when drugs are in short supply.展开更多
The cyber physician will scan you now:how Al models are enhancing diagnostics and treatment in hospitals,After studying a MRI test on February 13 at Beijing Children^Hospital(BCH),13 top pediatricians were surprised w...The cyber physician will scan you now:how Al models are enhancing diagnostics and treatment in hospitals,After studying a MRI test on February 13 at Beijing Children^Hospital(BCH),13 top pediatricians were surprised when the country's first Al pediatrician came to an identical conclusion as theirs in the case of an 8-year-old boy who had been having seizures.展开更多
China announced on July 1 the creation of a specialized reimbursement catalogue for domestically developed novel drugs that had previously been too expensive to be covered by the country's basic medical insurance ...China announced on July 1 the creation of a specialized reimbursement catalogue for domestically developed novel drugs that had previously been too expensive to be covered by the country's basic medical insurance system.展开更多
Depressive disorders affect many Chinese seniors but are often underdiagnosed due to lack of mental health facilities and doctors,as well as social stigma.More resources are needed,say professionals.Henan native Zhang...Depressive disorders affect many Chinese seniors but are often underdiagnosed due to lack of mental health facilities and doctors,as well as social stigma.More resources are needed,say professionals.Henan native Zhang He,now at 62,did not realize that her weight loss was due to mental illness.Around three years ago,she lost 20 kilos in just six months,but she believed her illness was physical,she told NewsChina.展开更多
文摘建立了一种超高效液相色谱串联质谱法(UPLC-MS/MS)同时测定土壤中8种多溴联苯醚化合物(PBDEs)的方法,采用快速溶剂萃取(ASE)和凝胶净化色谱(GPC),经BEH反相C 18色谱柱分离,10 min内完成分离,使用含0.1%氨水的甲醇和水进行梯度洗脱,采用液相色谱-负离子-大气压化学电离(APCI)串联质谱,多反应监测模式(MRM),加入13 C 12-BDE-139和13 C 12-BDE-209内标作定量分析。结果表明:在优化的分析条件下,PBDEs的线性相关系数为0.9988~0.9995,方法检出限为0.22~0.59μg/kg,在2个添加水平下,平均回收率为71%~126%,标准偏差为3%~18%,土壤实际质控样测定结果均在允许范围内。适合于土壤中PBDEs的快速分析,特别对于高溴代PBDEs,相比气相色谱质谱方法具有更快的分析速度和更好的稳定性。
文摘建立离子色谱法同时测定环境空气和废气中二甲胺、二乙胺和三乙胺等挥发性胺类化合物的方法。以甲磺酸(MSA)-乙腈水溶液为吸收液,采集环境空气或废气中的挥发性胺类化合物,利用Metrosep C4-250/4.0阳离子交换柱分离目标化合物,881 compact IC pro 1电导检测器进行检测,以保留时间定性、峰面积定量。结果显示:二甲胺、二乙胺和三乙胺标准曲线线性关系良好,相关系数在0.9992~0.9999之间,废气中二甲胺、二乙胺和三乙胺的检出限分别为0.24,0.30和0.42 mg/m^(3);环境空气中二甲胺、二乙胺和三乙胺的检出限分别为0.06,0.10和0.14 mg/m~3。该方法样品无需复杂的前处理过程,操作简单、分析速度快、准确度高,可同时测定共存于环境空气和废气中的二甲胺、二乙胺和三乙胺。
基金the National Natural Science Foundation of China(82271304,81801150,81971121,82171316 and 81671167)the Science and Technology Planning Project of Guangdong Province,China(2017A020215049,2019A050513005)+6 种基金Natural Science Foundation of Guangdong Province(2018A0303130182,2020A1515010279 and 2022A1515012311)the Fundamental Research Funds for the Central Universities(21621102)Science and Technology Projects in Guangzhou,China(2014Y2-00505,202002020003,202201010127,and SL2023A03J01214)Science and Technology Program of Guangzhou:Key Lab of Guangzhou Basic and Translational Research of Pan-vascular Diseases(202201020042)Young Talent Support Project of Guangzhou Association for Science and Technology(QT-2023-024)Guangdong Basic and Applied Basic Research Foundation(2021A1515111226)China Postdoctoral Science Foundation(2022M710058).
文摘Few studies have investigated the properties and protein composition of small extracellular vesicles (sEVs) derived from neurons under hypoxic conditions. Presently, the extent of the involvement of these plentiful sEVs in the onset and progression of ischemic stroke remains an unresolved question. Our study systematically identified the characteristics of sEVs derived from neurons under hypoxic conditions (HypEVs) by physical characterization, sEV absorption, proteomics and transcriptomics analysis. The effects of HypEVs on neurites, cell survival, and neuron structure were assessed in vitro and in vivo by neural complexity tests, magnetic resonance imaging (MRI), Golgi staining, and Western blotting of synaptic plasticity-related proteins and apoptotic proteins. Knockdown of Fused in Sarcoma (FUS) small interfering RNA (siRNA) was used to validate FUS-mediated HypEV neuroprotection and mitochondrial mRNA release. Hypoxia promoted the secretion of sEVs, and HypEVs were more easily taken up and utilized by recipient cells. The MRI results illustrated that the cerebral infarction volume was reduced by 45% with the application of HypEVs, in comparison to the non- HypEV treatment group. Mechanistically, the FUS protein is necessary for the uptake and neuroprotection of HypEVs against ischemic stroke as well as carrying a large amount of mitochondrial mRNA in HypEVs. However, FUS knockdown attenuated the neuroprotective rescue capabilities of HypEVs. Our comprehensive dataset clearly illustrates that FUS-mediated HypEVs deliver exceptional neuroprotective effects against ischemic stroke, primarily through the maintenance of neurite integrity and the reduction of mitochondria-associated apoptosis.
文摘Experts and patients are calling for a national blood donation system after some patients were forced to wait for treatment due to the fragmented system which does not recognize donations across different regions。
文摘China is getting tough on Big Pharma's monopolistic practices,slapping huge fines on firms that corner the market in crucial medications.Experts question whether fines are enough to deter the shady behavior when drugs are in short supply.
文摘The cyber physician will scan you now:how Al models are enhancing diagnostics and treatment in hospitals,After studying a MRI test on February 13 at Beijing Children^Hospital(BCH),13 top pediatricians were surprised when the country's first Al pediatrician came to an identical conclusion as theirs in the case of an 8-year-old boy who had been having seizures.
文摘China announced on July 1 the creation of a specialized reimbursement catalogue for domestically developed novel drugs that had previously been too expensive to be covered by the country's basic medical insurance system.
文摘Depressive disorders affect many Chinese seniors but are often underdiagnosed due to lack of mental health facilities and doctors,as well as social stigma.More resources are needed,say professionals.Henan native Zhang He,now at 62,did not realize that her weight loss was due to mental illness.Around three years ago,she lost 20 kilos in just six months,but she believed her illness was physical,she told NewsChina.
