Objective To investigate the effect of Coriolus versicolor polysaccharide-B （CVPs-B） on the biological characteristics of human esophageal carcinoma cell line Ecal09 in vitro. Methods The cells of experimental group...Objective To investigate the effect of Coriolus versicolor polysaccharide-B （CVPs-B） on the biological characteristics of human esophageal carcinoma cell line Ecal09 in vitro. Methods The cells of experimental group （EG） were cultured in DMEM with 10% FCS and 150μg/mL CVPs-B, the cells of control group （CG） were cultured in DMEM with 10% FCS without CVPs-B. MTT reduction assay was performed to detect the effect of CVPs-B on the proliferation of Ecal09 cells after the compound was administrated in varying concentrations. The living conditions of the Ecal09 cells were determined using trypan blue exclusion. Then, cell growth curves were drawn. Flow cytometry was performed to detect the effect of CVPs-B on the apoptosis and cell cycle of Ecal09. Results In comparison with the CG, a marked decrease in the proliferation of Eca09 cells was observed in the EG, after incubation with CVPs-B. The survival rate of Eca09 cells decreased as the time of CVPs-B incubation prolonged. Comparing the cell cycles and apoptotic rates between the two groups, the proportions of cells in the G0/G1, S, and G2/M phases in the EG were found to be （68.4±3.7）%, （13.9±2.1）%, and （17.7±1.4）%, respectively, after 24 h incubation with CVPs-B. The cells had an apoptotic rate of （9.7±0.7）%. On the other hand, the proportions of the G0/G1, S, and G2/M cells of the CG were found to be （53.9±3.6）%, （26.6±2.8）%, and （19.5±2.3）%, respectively, with an apoptotic rate of （5.7±1.4）%. In comparison with the CG cells, significant cell growth in the G0/G1 phase was observed in the EG （P〈0.05）. Furthermore, a significant decrease in the number of cells in the S phase was observed （P〈0.05） in the EG. Conclusions CVPs-B can inhibit proliferation and enhance apoptosis of Ecal09 cells and may be useful in the treatment of esophageal carcinoma.展开更多
Two new sesquiterpene esters, 3b, 6b, 8a-triacetyl-4b, 5a-epoxy -1- oxogermacr- 10(14)-ene (1) and 3b, 6b, 8a-triacetyl-4b, 5a-epoxygermacr-1(10)-ene (2) were isolated from the whole plant of Salvia roborowskii Maxim....Two new sesquiterpene esters, 3b, 6b, 8a-triacetyl-4b, 5a-epoxy -1- oxogermacr- 10(14)-ene (1) and 3b, 6b, 8a-triacetyl-4b, 5a-epoxygermacr-1(10)-ene (2) were isolated from the whole plant of Salvia roborowskii Maxim. Their structures were elucidated by means of spectral data (2DNMR and HRMS).展开更多
Current subtyping methods of diffuse large B-cell lymphoma(DLBCL)could not satisfy the clinical demands for risk assessment and prognostic prediction.We aimed to investigate the prognostic effect of autophagy-related ...Current subtyping methods of diffuse large B-cell lymphoma(DLBCL)could not satisfy the clinical demands for risk assessment and prognostic prediction.We aimed to investigate the prognostic effect of autophagy-related genes(ARGs)in DLBCL.Transcriptomic data of 1,409 DLBCL patients,531 healthy controls(HCs),and single-cell sequencing data of 4 DLBCL were included.Validation involved spatial transcriptomics from 10 DLBCL patients and 110 DLBCL proteomic data from a local cohort.We identified 153 differentially expressed ARGs between DLBCL patients(n=48)and HCs(n=531),classifying 414 DLBCL patients into two subtypes based on autophagy heterogeneity.Subtype I,characterized by upregulated T regulatory(Treg)cells(P<0.0001)and T follicular helper(Tfh)cells(P=0.0012),showed a superior prognosis(P=0.035).Eight prognostic ARGs were selected to construct an autophagy-related model,dividing patients into low-and high-risk groups.Kaplan-Meier survival analysis revealed significantly better outcomes for the low-risk group in both the discovery(P<0.0001)and validation cohorts(P=0.0041).High-risk patients exhibited elevated IDO1(P=0.042)and LAG3(P<0.001)levels.Among the eight signature proteins,higher FAS was further verified to indicate a better prognosis in the local cohort(n=110)using antibody array(P=0.0083).FAS was primarily expressed in T cells such as Treg and Tfh cells and was elevated in non-progressive disease patients.FASpositive T cells showed increased interferon-gamma(normalized enrichment score(NES)=2.196,FDR<0.0001)and alpha(NES=1.836,FDR<0.01)response activities.We constructed an autophagy-related model and identified FAS as a prognostic biomarker.FAS+Treg and Tfh cell-enriched TME indicated a favorable prognosis.展开更多
基金supported by the Guangdong Provincial Sci-Tech Planning(No.2010B030700051)
文摘Objective To investigate the effect of Coriolus versicolor polysaccharide-B （CVPs-B） on the biological characteristics of human esophageal carcinoma cell line Ecal09 in vitro. Methods The cells of experimental group （EG） were cultured in DMEM with 10% FCS and 150μg/mL CVPs-B, the cells of control group （CG） were cultured in DMEM with 10% FCS without CVPs-B. MTT reduction assay was performed to detect the effect of CVPs-B on the proliferation of Ecal09 cells after the compound was administrated in varying concentrations. The living conditions of the Ecal09 cells were determined using trypan blue exclusion. Then, cell growth curves were drawn. Flow cytometry was performed to detect the effect of CVPs-B on the apoptosis and cell cycle of Ecal09. Results In comparison with the CG, a marked decrease in the proliferation of Eca09 cells was observed in the EG, after incubation with CVPs-B. The survival rate of Eca09 cells decreased as the time of CVPs-B incubation prolonged. Comparing the cell cycles and apoptotic rates between the two groups, the proportions of cells in the G0/G1, S, and G2/M phases in the EG were found to be （68.4±3.7）%, （13.9±2.1）%, and （17.7±1.4）%, respectively, after 24 h incubation with CVPs-B. The cells had an apoptotic rate of （9.7±0.7）%. On the other hand, the proportions of the G0/G1, S, and G2/M cells of the CG were found to be （53.9±3.6）%, （26.6±2.8）%, and （19.5±2.3）%, respectively, with an apoptotic rate of （5.7±1.4）%. In comparison with the CG cells, significant cell growth in the G0/G1 phase was observed in the EG （P〈0.05）. Furthermore, a significant decrease in the number of cells in the S phase was observed （P〈0.05） in the EG. Conclusions CVPs-B can inhibit proliferation and enhance apoptosis of Ecal09 cells and may be useful in the treatment of esophageal carcinoma.
基金the Natural Science Foundation of Gansu Province(No.25001-A25-001-2)
文摘Two new sesquiterpene esters, 3b, 6b, 8a-triacetyl-4b, 5a-epoxy -1- oxogermacr- 10(14)-ene (1) and 3b, 6b, 8a-triacetyl-4b, 5a-epoxygermacr-1(10)-ene (2) were isolated from the whole plant of Salvia roborowskii Maxim. Their structures were elucidated by means of spectral data (2DNMR and HRMS).
基金supported by Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(CIFMS 2021-12M-1003)National High Level Hospital Clinical Research Funding(2022-PUMCH-D-002)China National Major Project for New Drug Innovation(2019ZX09201-002)。
文摘Current subtyping methods of diffuse large B-cell lymphoma(DLBCL)could not satisfy the clinical demands for risk assessment and prognostic prediction.We aimed to investigate the prognostic effect of autophagy-related genes(ARGs)in DLBCL.Transcriptomic data of 1,409 DLBCL patients,531 healthy controls(HCs),and single-cell sequencing data of 4 DLBCL were included.Validation involved spatial transcriptomics from 10 DLBCL patients and 110 DLBCL proteomic data from a local cohort.We identified 153 differentially expressed ARGs between DLBCL patients(n=48)and HCs(n=531),classifying 414 DLBCL patients into two subtypes based on autophagy heterogeneity.Subtype I,characterized by upregulated T regulatory(Treg)cells(P<0.0001)and T follicular helper(Tfh)cells(P=0.0012),showed a superior prognosis(P=0.035).Eight prognostic ARGs were selected to construct an autophagy-related model,dividing patients into low-and high-risk groups.Kaplan-Meier survival analysis revealed significantly better outcomes for the low-risk group in both the discovery(P<0.0001)and validation cohorts(P=0.0041).High-risk patients exhibited elevated IDO1(P=0.042)and LAG3(P<0.001)levels.Among the eight signature proteins,higher FAS was further verified to indicate a better prognosis in the local cohort(n=110)using antibody array(P=0.0083).FAS was primarily expressed in T cells such as Treg and Tfh cells and was elevated in non-progressive disease patients.FASpositive T cells showed increased interferon-gamma(normalized enrichment score(NES)=2.196,FDR<0.0001)and alpha(NES=1.836,FDR<0.01)response activities.We constructed an autophagy-related model and identified FAS as a prognostic biomarker.FAS+Treg and Tfh cell-enriched TME indicated a favorable prognosis.
