Raman imaging has the capability to provide unlabeled,spatially aware analysis of chemical components,with no a priori assumptions.Several lifestyle diseases such as nonalcoholic steatohepatitis(NASH)can appear in the...Raman imaging has the capability to provide unlabeled,spatially aware analysis of chemical components,with no a priori assumptions.Several lifestyle diseases such as nonalcoholic steatohepatitis(NASH)can appear in the liver as changes in the nature,abundance,and distribution of lipids,proteins,and other biomolecules and are detectable by Raman imaging.In order to identify which of these liver-associated changes occur as a direct result of the diet and which are secondary effects,we developed correlative imaging and analysis of diet and liver samples.Oleic acid was found to be a direct contributor to NASH liver composition,whereas protein and collagen distributions were found to be affected in a manner consistent with early fibrotic transformation,as a secondary consequence of the high-fat diet.展开更多
基金funded by the Japan Society for the Promotion of Science(JSPS)through the Funding Program for World-Leading Innovative R&D on Science and Technology(FIRST Program)the JSPS World Premier International Research Center Initiative Funding Programthe Uehara Memorial Foundation.S.A.acknowledges funding from JSPS KAKENHI Grant-in-Aid for Scientific Research S(20H05693).
文摘Raman imaging has the capability to provide unlabeled,spatially aware analysis of chemical components,with no a priori assumptions.Several lifestyle diseases such as nonalcoholic steatohepatitis(NASH)can appear in the liver as changes in the nature,abundance,and distribution of lipids,proteins,and other biomolecules and are detectable by Raman imaging.In order to identify which of these liver-associated changes occur as a direct result of the diet and which are secondary effects,we developed correlative imaging and analysis of diet and liver samples.Oleic acid was found to be a direct contributor to NASH liver composition,whereas protein and collagen distributions were found to be affected in a manner consistent with early fibrotic transformation,as a secondary consequence of the high-fat diet.
