Objective:To investigate the protective effect of alpha-lipoic acid on lead-induced Sertoli cell injury and its potential molecular mechanisms using primary Sertoli cells from rooster testes.Methods:Primary Sertoli ce...Objective:To investigate the protective effect of alpha-lipoic acid on lead-induced Sertoli cell injury and its potential molecular mechanisms using primary Sertoli cells from rooster testes.Methods:Primary Sertoli cells from 6-week-old rooster testes were divided into four goups.They were treated with serum-free DMEM-F12 medium(the control group),40μM lead acetate,100μM alpha-lipoic acid,or 40μM lead acetate plus 100μM alpha-lipoic acid for 24 h,respectively.Cell viability,reactive oxygen species levels(DCFH-DA),antioxidant enzyme activities(catalase,superoxide dismutase),blood-testis barrier-related proteins,nuclear factor-erythroid 2-related factor 2(Nrf2)pathway components,proliferation/apoptosis markers(Western blot),and related gene expression(qPCR)were analyzed.Results:Alpha-lipoic acid treatment significantly reduced lead-induced reactive oxygen species(ROS)production by 58.3%compared to the lead acetate group(P<0.05),enhanced catalase activity by 35.8%(P<0.05),and elevated lead-reduced PCNA expression by 47.2%(P<0.05).Lead acetate 40μM plus 100μM alpha-lipoic acid significantly increased Nrf2 expression by 78.9%and HO1/NQO1 expression by 97.5%and 89.4%,respectively(P<0.05),significantly upregulated the expression of Bcl2 protein and significantly downregulated Bax protein expression(P<0.05).Conclusions:Alpha-lipoic acid enhances the antioxidant properties of Sertoli cells by activating the Nrf2 pathway,restores lead-induced excessive autophagy,and inhibits apoptosis,thus alleviating the damage caused by lead in Sertoli cells.展开更多
基金funded by Zhejiang Provincial Natural Science Foundation of China under Grant No.LTGN24C170001Zhejiang Province Vocational Education Teaching Reform Project jg20230038+1 种基金Zhejiang Province Education Science Planning Project 2024JCD034Jinhua Science and Technology Plan Project 2024-2-002.
文摘Objective:To investigate the protective effect of alpha-lipoic acid on lead-induced Sertoli cell injury and its potential molecular mechanisms using primary Sertoli cells from rooster testes.Methods:Primary Sertoli cells from 6-week-old rooster testes were divided into four goups.They were treated with serum-free DMEM-F12 medium(the control group),40μM lead acetate,100μM alpha-lipoic acid,or 40μM lead acetate plus 100μM alpha-lipoic acid for 24 h,respectively.Cell viability,reactive oxygen species levels(DCFH-DA),antioxidant enzyme activities(catalase,superoxide dismutase),blood-testis barrier-related proteins,nuclear factor-erythroid 2-related factor 2(Nrf2)pathway components,proliferation/apoptosis markers(Western blot),and related gene expression(qPCR)were analyzed.Results:Alpha-lipoic acid treatment significantly reduced lead-induced reactive oxygen species(ROS)production by 58.3%compared to the lead acetate group(P<0.05),enhanced catalase activity by 35.8%(P<0.05),and elevated lead-reduced PCNA expression by 47.2%(P<0.05).Lead acetate 40μM plus 100μM alpha-lipoic acid significantly increased Nrf2 expression by 78.9%and HO1/NQO1 expression by 97.5%and 89.4%,respectively(P<0.05),significantly upregulated the expression of Bcl2 protein and significantly downregulated Bax protein expression(P<0.05).Conclusions:Alpha-lipoic acid enhances the antioxidant properties of Sertoli cells by activating the Nrf2 pathway,restores lead-induced excessive autophagy,and inhibits apoptosis,thus alleviating the damage caused by lead in Sertoli cells.
