Marine microbial-derived exopolysaccharides(EPS)have gradually gained popularity as function ingredients in the food industry because of their unique structures and various biological functions.EPS-LAN4 is a novel EPS...Marine microbial-derived exopolysaccharides(EPS)have gradually gained popularity as function ingredients in the food industry because of their unique structures and various biological functions.EPS-LAN4 is a novel EPS derived from the Limosilactobacillus fermentum LAN4,which is a derivative of marine lactobacilli.Its bacteria can stimulate the immune system,enhance the resistance of marine organisms to diseases,and reduce the risk of infection caused by pathogenic bacteria.In previous studies,it has been determined that EPS-LAN4 has a relatively small average molecular weight(13.52 kDa)and contains bothα-glycosidic andβ-glycosidic bonds,making it a highly water-soluble and bioavailable polysaccharide.In this study,it was demonstrated that EPS-LAN4 protects HUVECs from H_(2)O_(2)-induced effects by inhibiting the production of intracellular reactive oxy-gen species(ROS),blocking p53 mediated Caspase-3/-9 activation and activating the Keap1-Nrf2 pathway,thereby inhibiting cell apoptosis.In addition,during the activity research process,it was found that EPS not only has good antioxidant activity,but also excellent anti-angiogenic activity,and its synergistic effect is related to the structural characteristics of EPS.The results of this study provide new nutritional resources for the development of functional components for chronic metabolic disorders such as cardiovascular diseases,and also open up new avenues for research in the field of microbial polysaccharides.展开更多
基金supported by the Guangdong Basic and Applied Basic Research Foundation(2023A1515010005)Special funds for universities in Guangdong Province in the key areas of biomedicine and health(2023ZDZX2025)the Innovative Team Program of High Education of Guangdong Province(2021KCXTD021).
文摘Marine microbial-derived exopolysaccharides(EPS)have gradually gained popularity as function ingredients in the food industry because of their unique structures and various biological functions.EPS-LAN4 is a novel EPS derived from the Limosilactobacillus fermentum LAN4,which is a derivative of marine lactobacilli.Its bacteria can stimulate the immune system,enhance the resistance of marine organisms to diseases,and reduce the risk of infection caused by pathogenic bacteria.In previous studies,it has been determined that EPS-LAN4 has a relatively small average molecular weight(13.52 kDa)and contains bothα-glycosidic andβ-glycosidic bonds,making it a highly water-soluble and bioavailable polysaccharide.In this study,it was demonstrated that EPS-LAN4 protects HUVECs from H_(2)O_(2)-induced effects by inhibiting the production of intracellular reactive oxy-gen species(ROS),blocking p53 mediated Caspase-3/-9 activation and activating the Keap1-Nrf2 pathway,thereby inhibiting cell apoptosis.In addition,during the activity research process,it was found that EPS not only has good antioxidant activity,but also excellent anti-angiogenic activity,and its synergistic effect is related to the structural characteristics of EPS.The results of this study provide new nutritional resources for the development of functional components for chronic metabolic disorders such as cardiovascular diseases,and also open up new avenues for research in the field of microbial polysaccharides.
