Clustered regularly interspaced short palindromic repeats(CRISPR)is an adaptive immune system in microorganisms,utilizing CRISPR RNA(crRNA)and CRISPR-associated proteins(Cas)to eliminate invasive nucleic acids[1].Due ...Clustered regularly interspaced short palindromic repeats(CRISPR)is an adaptive immune system in microorganisms,utilizing CRISPR RNA(crRNA)and CRISPR-associated proteins(Cas)to eliminate invasive nucleic acids[1].Due to its simplicity,programmability,precise targeting,and efficient cleavage abilities,the CRISPR/Cas system has become a highly effective diagnostic tool[2].As a prominent system in CRISPR-based diagnostics,CRISPR/Cas12a enables Cas12a to cleave the target DNA(ciscleavage)and continuously trans-cleave non-specific singlestranded DNA(ssDNA)following crRNA-mediated binding to the target DNA[3,4].展开更多
基金supported by the Natural Science Foundation of Xiamen,China(3502Z202373019)National Key R&D Program of China(2023YFD1800502)+5 种基金National Natural Science Foundation of China(31971369)Natural Science Foundation of Fujian Province(2019J02004)CAMS Innovation Fund for Medical Sciences(2019RU022)the Fundamental Research Funds for the Central Universities(20720220006)the Fundamental Research Funds for the Central Universities(20720220005)the Science and Technology Planning Project of Guangdong Province of China(B1212030009)。
文摘Clustered regularly interspaced short palindromic repeats(CRISPR)is an adaptive immune system in microorganisms,utilizing CRISPR RNA(crRNA)and CRISPR-associated proteins(Cas)to eliminate invasive nucleic acids[1].Due to its simplicity,programmability,precise targeting,and efficient cleavage abilities,the CRISPR/Cas system has become a highly effective diagnostic tool[2].As a prominent system in CRISPR-based diagnostics,CRISPR/Cas12a enables Cas12a to cleave the target DNA(ciscleavage)and continuously trans-cleave non-specific singlestranded DNA(ssDNA)following crRNA-mediated binding to the target DNA[3,4].
