Bacterial wound infections are a growing challenge in healthcare,posing severe risks like systemic infection,organ failure,and sepsis,with projections predicting over 10 million deaths annually by 2050.Antibacterial h...Bacterial wound infections are a growing challenge in healthcare,posing severe risks like systemic infection,organ failure,and sepsis,with projections predicting over 10 million deaths annually by 2050.Antibacterial hydrogels,with adaptable extracellular matrix-like features,are emerging as promising solutions for treating infectious wounds.However,the antibacterial properties of most of these hydrogels are largely attributed to extrinsic agents,and their mechanisms of action remain poorly understood.Herein we introduce for the first time,modified imidazolidinyl urea(IU)as the polymeric backbone for developing tissue-like antibacterial hydrogels.As-designed hydrogels behave tissue-like mechanical features,outstanding antifreeze behavior,and rapid self-healing capabilities.Molecular dynamics(MD)simulation and density functional theory(DFT)calculation were employed to well-understand the extent of H-bonding and metal-ligand coordination to finetune hydrogels’properties.In vitro studies suggest good biocompatibility of hydrogels against mouse fibroblasts&human skin,lung,and red blood cells,with potential wound healing capacity.Additionally,the hydrogels exhibit good 3D printability and remarkable antibacterial activity,attributed to concentration dependent ROS genera-tion,oxidative stress induction,and subsequent disruption of bacterial membrane.On top of that,in vitro biofilm studies confirmed that developed hydrogels are effective in preventing biofilm formation.Therefore,these tissue-mimetic hydrogels present a promising and effective platform for accelerating wound healing while simulta-neously controlling bacterial infections,offering hope for the future of wound care.展开更多
基金supported by MAOF Fellowship from the Council for Higher Education,Israel.K.G.acknowledges Aly Kaufman Fellowship Trust for partial support of his fellowshipsupported by the Israel Science Foundation and the Technion’s president grant,and we thank them for that.
文摘Bacterial wound infections are a growing challenge in healthcare,posing severe risks like systemic infection,organ failure,and sepsis,with projections predicting over 10 million deaths annually by 2050.Antibacterial hydrogels,with adaptable extracellular matrix-like features,are emerging as promising solutions for treating infectious wounds.However,the antibacterial properties of most of these hydrogels are largely attributed to extrinsic agents,and their mechanisms of action remain poorly understood.Herein we introduce for the first time,modified imidazolidinyl urea(IU)as the polymeric backbone for developing tissue-like antibacterial hydrogels.As-designed hydrogels behave tissue-like mechanical features,outstanding antifreeze behavior,and rapid self-healing capabilities.Molecular dynamics(MD)simulation and density functional theory(DFT)calculation were employed to well-understand the extent of H-bonding and metal-ligand coordination to finetune hydrogels’properties.In vitro studies suggest good biocompatibility of hydrogels against mouse fibroblasts&human skin,lung,and red blood cells,with potential wound healing capacity.Additionally,the hydrogels exhibit good 3D printability and remarkable antibacterial activity,attributed to concentration dependent ROS genera-tion,oxidative stress induction,and subsequent disruption of bacterial membrane.On top of that,in vitro biofilm studies confirmed that developed hydrogels are effective in preventing biofilm formation.Therefore,these tissue-mimetic hydrogels present a promising and effective platform for accelerating wound healing while simulta-neously controlling bacterial infections,offering hope for the future of wound care.
