A case of imported severe falciparum malaria with spontaneous splenic rupture was reported in this paper.The patient,an African migrant worker,developed hemolytic anemia,sepsis,thrombocytopenia,coagulation dysfunction...A case of imported severe falciparum malaria with spontaneous splenic rupture was reported in this paper.The patient,an African migrant worker,developed hemolytic anemia,sepsis,thrombocytopenia,coagulation dysfunction,liver failure,renal insufficiency,electrolyte disturbance and other clinical manifestations after returning to the local area.Plasmodium falciparum was found by peripheral blood smearscopy and was diagnosed as severe falciparum malaria.After standardized anti-malaria treatment,plasma exchange+cytokine adsorption therapy,the establishment of“forewarning-forewarning-prevention-emergency”predictive nursing management model,the establishment of an integrated nursing team,the division of medical care is clear,professional knowledge is complementary,after three months of regular follow-up,the patient has no malaria recurrence,no refire,the function of all organs returned to normal.展开更多
Objective： A direct-current, cold atmospheric-pressure air plasma microjet （PMJ） was performed to inactivate Staphylococcus aureus （S. aureus） and Enterococcusfaecalis （E. faecalis） in air. The process of steri...Objective： A direct-current, cold atmospheric-pressure air plasma microjet （PMJ） was performed to inactivate Staphylococcus aureus （S. aureus） and Enterococcusfaecalis （E. faecalis） in air. The process of sterilization and morphology of bacteria was observed. We wish to know the possible inactivation mechanisms of PMJ and explore a potential application in dental and other temperature sensitive treatment. Methods： In this study, we employed a direct current, atmospheric pressure, cold air PMJ to inactivate bacterias. Scanning electron microscopy was employed to evaluate the morphology of S. aureus and showed rupture of cell walls after the plasma treatment and Optical emission spectrum （OES） were used to understand the possible inactivation mechanisms of PMJ. Re- suits＂ The inactivation rates could reach 100% in 5 min. When the distance between the exit nozzle of the PMJ device and Petri dish was extended from 1 cm to 3 cm, effective inactivation was also observed with a similar inactivation curve. Conclusion： The inactivation of bacteria is attributed to the abundant reactive oxygen and nitrogen species, as well as ultroviolet radiation in the plasma. Different life spans and defensibilities of these killing agents may hold the key to understanding the different inactivation curves at different treatment distances.展开更多
In this paper, the petal-like MoS2/MXene composite has been successfully synthesized by one-step hydrothermal method. With the combination of few-layer MoS2nanosheets and the high conductive MXene substrate, the compo...In this paper, the petal-like MoS2/MXene composite has been successfully synthesized by one-step hydrothermal method. With the combination of few-layer MoS2nanosheets and the high conductive MXene substrate, the composite exhibits enhanced capacities of 165 mAh/g at 50 mA/g and outstanding rate performance of 93 mAh/g at 200 mA/g as a cathode material of Mg batteries. Simultaneously, MXene with high conductivity and abundant surface functional groups is proved to be a promising substrate for the wider design of high performance electrode materials.展开更多
Predatory fungi possess intricate signal transduction systems that regulate their development and support successful infection of the host.Herein,we characterized three components of the cell wall integrity-controllin...Predatory fungi possess intricate signal transduction systems that regulate their development and support successful infection of the host.Herein,we characterized three components of the cell wall integrity-controlling pathway,namely protein kinase C(Ao PKC),SLT2-MAPK(Ao SLT2),and SWI6(Ao SWI6),in a representative nematode-trapping fungus Arthrobotrys oligospora,using gene disruption and multi-omics approaches.The phenotypic traits(such as mycelia development,conidiation,stress response,and trap morphogenesis) and metabolic profiles of ΔAopkc and ΔAoswi6 mutants were similar but differed from those of the ΔAoslt2 mutants.Transcriptomic analysis indicated that the genes differentially expressed in the absence of Aoswi6 were involved in DNA replication,repair,and recombination during trap formation.Moreover,the yeast two-hybrid assay showed that Ao PKC interacted with Ao SWI6,suggesting that in A.oligospora,PKC can directly regulate SWI6,bypassing the SLT2signaling cascade.Conclusively,our findings deepen our understanding of the regulatory mechanism of asexual development and lifestyle switching in nematode-trapping fungi.展开更多
Autophagy is an evolutionarily conserved process in eukaryotes,which is regulated by autophagy-related genes(ATGs).Arthrobotrys oligospora is a representative species of nematode-trapping(NT)fungi that can produce spe...Autophagy is an evolutionarily conserved process in eukaryotes,which is regulated by autophagy-related genes(ATGs).Arthrobotrys oligospora is a representative species of nematode-trapping(NT)fungi that can produce special traps for nematode predation.To elucidate the biological roles of autophagy in NT fungi,we characterized an orthologous Atg protein,AoAtg5,in A.oligospora.We found that AoATG5 deletion causes a significant reduction in vegetative growth and conidiation,and that the transcript levels of several sporulation-related genes were significantly downregulated during sporulation stage.In addition,the cell nuclei were significantly reduced in theΔAoATG5 mutant,and the transcripts of several genes involved in DNA bio-synthesis,repair,and ligation were significantly upregulated.InΔAoATG5 mutants,the autophagic process was significantly impaired,and trap formation and nematocidal activity were significantly decreased.Comparative transcriptome analysis results showed that AoAtg5 is involved in the regulation of multiple cellular processes,such as autophagy,nitrogen metabolism,DNA biosynthesis and repair,and vesicular transport.In summary,our results suggest that AoAtg5 is essential for autophagy and significantly contributes to vegetative growth,cell nucleus development,sporulation,trap formation,and pathogenicity in A.oligospora,thus providing a basis for future studies focusing on related mechanisms of autophagy in NT fungi.展开更多
In the paper,we want to derive a few of nonlinear Schrodinger equations with various formats and investigate their properties,such as symmetries,single soliton solutions,multi-soliton solutions,and so on.First of all,...In the paper,we want to derive a few of nonlinear Schrodinger equations with various formats and investigate their properties,such as symmetries,single soliton solutions,multi-soliton solutions,and so on.First of all,we propose an efficient and straightforward scheme for generating nonisospectral integrable hierarchies of evolution equations for which a generalized nonisospectral integrable Schrodinger hierarchy(briefly GNISH)singles out,from which we get a derivative nonlinear Schrodinger equation,a generalized nonlocal Schrodinger integrable system and furthermore we investigate the symmetries and conserved qualities of the GNISH.Next,we apply the dbar method to obtain a generalized nonlinear Schr?dinger-Maxwell-Bloch(GNLS-MB)equation and its hierarchy by introducing a generalized Zakhrov-Shabat spectral problem,whose soliton solutions and gauge transformations are obtained.展开更多
基金“Artificial Liver Special Fund”of Beijing Gan Dan Xiang Zhao Public Welfare Foundation(Project No.:iGandanF-1082024-RGG055)。
文摘A case of imported severe falciparum malaria with spontaneous splenic rupture was reported in this paper.The patient,an African migrant worker,developed hemolytic anemia,sepsis,thrombocytopenia,coagulation dysfunction,liver failure,renal insufficiency,electrolyte disturbance and other clinical manifestations after returning to the local area.Plasmodium falciparum was found by peripheral blood smearscopy and was diagnosed as severe falciparum malaria.After standardized anti-malaria treatment,plasma exchange+cytokine adsorption therapy,the establishment of“forewarning-forewarning-prevention-emergency”predictive nursing management model,the establishment of an integrated nursing team,the division of medical care is clear,professional knowledge is complementary,after three months of regular follow-up,the patient has no malaria recurrence,no refire,the function of all organs returned to normal.
基金supported by Bioelectrics Inc.(USA),Peking University Biomed-X Foundation and China International Science and Technology Cooperation(No.2008KR1330)
文摘Objective： A direct-current, cold atmospheric-pressure air plasma microjet （PMJ） was performed to inactivate Staphylococcus aureus （S. aureus） and Enterococcusfaecalis （E. faecalis） in air. The process of sterilization and morphology of bacteria was observed. We wish to know the possible inactivation mechanisms of PMJ and explore a potential application in dental and other temperature sensitive treatment. Methods： In this study, we employed a direct current, atmospheric pressure, cold air PMJ to inactivate bacterias. Scanning electron microscopy was employed to evaluate the morphology of S. aureus and showed rupture of cell walls after the plasma treatment and Optical emission spectrum （OES） were used to understand the possible inactivation mechanisms of PMJ. Re- suits＂ The inactivation rates could reach 100% in 5 min. When the distance between the exit nozzle of the PMJ device and Petri dish was extended from 1 cm to 3 cm, effective inactivation was also observed with a similar inactivation curve. Conclusion： The inactivation of bacteria is attributed to the abundant reactive oxygen and nitrogen species, as well as ultroviolet radiation in the plasma. Different life spans and defensibilities of these killing agents may hold the key to understanding the different inactivation curves at different treatment distances.
文摘In this paper, the petal-like MoS2/MXene composite has been successfully synthesized by one-step hydrothermal method. With the combination of few-layer MoS2nanosheets and the high conductive MXene substrate, the composite exhibits enhanced capacities of 165 mAh/g at 50 mA/g and outstanding rate performance of 93 mAh/g at 200 mA/g as a cathode material of Mg batteries. Simultaneously, MXene with high conductivity and abundant surface functional groups is proved to be a promising substrate for the wider design of high performance electrode materials.
基金supported by the National Natural Science Foundation of China(31960556,U1402265,32160665)the Applied Basic Research Foundation of Yunnan Province(202001BB050004)Postdoctoral Science Foundation of Yunnan Province。
文摘Predatory fungi possess intricate signal transduction systems that regulate their development and support successful infection of the host.Herein,we characterized three components of the cell wall integrity-controlling pathway,namely protein kinase C(Ao PKC),SLT2-MAPK(Ao SLT2),and SWI6(Ao SWI6),in a representative nematode-trapping fungus Arthrobotrys oligospora,using gene disruption and multi-omics approaches.The phenotypic traits(such as mycelia development,conidiation,stress response,and trap morphogenesis) and metabolic profiles of ΔAopkc and ΔAoswi6 mutants were similar but differed from those of the ΔAoslt2 mutants.Transcriptomic analysis indicated that the genes differentially expressed in the absence of Aoswi6 were involved in DNA replication,repair,and recombination during trap formation.Moreover,the yeast two-hybrid assay showed that Ao PKC interacted with Ao SWI6,suggesting that in A.oligospora,PKC can directly regulate SWI6,bypassing the SLT2signaling cascade.Conclusively,our findings deepen our understanding of the regulatory mechanism of asexual development and lifestyle switching in nematode-trapping fungi.
基金the National Natural Science Foundation of China(31960556 and U1402265)the Applied Basic Research Foundation of Yunnan Province(202001BB050004)the National Basic Research Program of China(2013CB127503)。
文摘Autophagy is an evolutionarily conserved process in eukaryotes,which is regulated by autophagy-related genes(ATGs).Arthrobotrys oligospora is a representative species of nematode-trapping(NT)fungi that can produce special traps for nematode predation.To elucidate the biological roles of autophagy in NT fungi,we characterized an orthologous Atg protein,AoAtg5,in A.oligospora.We found that AoATG5 deletion causes a significant reduction in vegetative growth and conidiation,and that the transcript levels of several sporulation-related genes were significantly downregulated during sporulation stage.In addition,the cell nuclei were significantly reduced in theΔAoATG5 mutant,and the transcripts of several genes involved in DNA bio-synthesis,repair,and ligation were significantly upregulated.InΔAoATG5 mutants,the autophagic process was significantly impaired,and trap formation and nematocidal activity were significantly decreased.Comparative transcriptome analysis results showed that AoAtg5 is involved in the regulation of multiple cellular processes,such as autophagy,nitrogen metabolism,DNA biosynthesis and repair,and vesicular transport.In summary,our results suggest that AoAtg5 is essential for autophagy and significantly contributes to vegetative growth,cell nucleus development,sporulation,trap formation,and pathogenicity in A.oligospora,thus providing a basis for future studies focusing on related mechanisms of autophagy in NT fungi.
基金supported by the National Natural Science Foundation of China(No.11971475)。
文摘In the paper,we want to derive a few of nonlinear Schrodinger equations with various formats and investigate their properties,such as symmetries,single soliton solutions,multi-soliton solutions,and so on.First of all,we propose an efficient and straightforward scheme for generating nonisospectral integrable hierarchies of evolution equations for which a generalized nonisospectral integrable Schrodinger hierarchy(briefly GNISH)singles out,from which we get a derivative nonlinear Schrodinger equation,a generalized nonlocal Schrodinger integrable system and furthermore we investigate the symmetries and conserved qualities of the GNISH.Next,we apply the dbar method to obtain a generalized nonlinear Schr?dinger-Maxwell-Bloch(GNLS-MB)equation and its hierarchy by introducing a generalized Zakhrov-Shabat spectral problem,whose soliton solutions and gauge transformations are obtained.
