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腐熟堆肥中维素降解菌筛选鉴定及酶学特性研究 被引量:15
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作者 周东兴 王广栋 +4 位作者 邬欣慧 宁玉翠 李晶 李磊 曹旭 《东北农业大学学报》 CAS CSCD 北大核心 2018年第5期60-68,共9页
为筛选高效纤维素降解菌,促进堆肥过程中秸秆等纤维素物质快速腐解,在以牛粪和秸秆为材料的腐熟堆肥中分离菌株,以刚果红培养基和滤纸条降解试验初筛菌株,筛选出透明圈与菌落直径比值较大、滤纸条分解能力较强的菌株,作液体发酵培养,测... 为筛选高效纤维素降解菌,促进堆肥过程中秸秆等纤维素物质快速腐解,在以牛粪和秸秆为材料的腐熟堆肥中分离菌株,以刚果红培养基和滤纸条降解试验初筛菌株,筛选出透明圈与菌落直径比值较大、滤纸条分解能力较强的菌株,作液体发酵培养,测定其酶活力,得到羧甲基纤维素(CMC)酶活和滤纸(FPA)酶活均较高2株菌(1号和7号),并将其在以羧甲基纤维素钠为唯一碳源产酶培养基中作产酶特性研究。结果表明,pH 6.5,培养时间48 h条件下,1号和7号菌株CMC酶活分别为26.82和31.28 U·m L-1,FPA酶活分别为20.32和20.82 U·m L-1。通过形态学、生理生化特征、16S rDNA核酸序列分析及26S rDNA的D1/D2区域测序鉴定,确定1号菌属于枯草芽孢杆菌(Bacillus subtilis),7号菌属于隐球酵母菌(Cryptococcus flavescens)。 展开更多
关键词 纤维素降解菌 枯草芽孢杆菌 隐球酵母菌 CMC酶活 FPA酶活
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蚯蚓粪配施化肥对稻田土壤性状和酶活的影响 被引量:28
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作者 周东兴 李欣 +4 位作者 宁玉翠 梁晓艳 王嘉昊 李云飞 刘煜 《东北农业大学学报》 CAS CSCD 北大核心 2021年第2期25-35,共11页
为降低化肥污染和改善稻田土壤肥力,试验施用不同剂量蚯蚓粪有机肥配施化肥,探究对种植水稻东北黑土土壤性状(碱解氮、全磷、速效磷和速效钾)和5种酶活(脱氢酶、磷酸酶、蔗糖酶、脲酶和过氧化氢酶)的影响,筛选适合水稻生长有机肥用量。... 为降低化肥污染和改善稻田土壤肥力,试验施用不同剂量蚯蚓粪有机肥配施化肥,探究对种植水稻东北黑土土壤性状(碱解氮、全磷、速效磷和速效钾)和5种酶活(脱氢酶、磷酸酶、蔗糖酶、脲酶和过氧化氢酶)的影响,筛选适合水稻生长有机肥用量。结果表明,8种施肥模式中,蚯蚓粪配施化肥处理土壤性状和酶活高于常规施肥对照组。经主成分分析,碱解氮、全磷、速效磷、脱氢酶和磷酸酶发挥主要作用,比较8种有机肥配施化肥土壤性状和酶活可知,化肥减量30%+蚯蚓粪(T3)处理效果最显著,与未施肥处理(CK)和常规化肥处理(CK0)相比,T3处理分别增产31.6%和71.8%(P<0.01),更利于促进水稻生长。 展开更多
关键词 稻田土壤 蚯蚓粪配施化肥 土壤性状 土壤酶活性 相关分析
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Estimation of the Effects of Maize Straw Return on Soil Carbon and Nutrients Using Response Surface Methodology 被引量:12
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作者 ZHOU Dongxing SU Ye +4 位作者 ning yucui RONG Guohua WANG Guangdong LIU Di LIU Liyan 《Pedosphere》 SCIE CAS CSCD 2018年第3期411-421,共11页
Straw incorporation is generally considered an effective agricultural management practice that improves nutrient cycling and maintains soil fertility. To study the interactive effects of straw returning factors on soi... Straw incorporation is generally considered an effective agricultural management practice that improves nutrient cycling and maintains soil fertility. To study the interactive effects of straw returning factors on soil organic carbon and available nutrients, a17-month(May 6, 2016 to October 6, 2017) experiment was conducted on straw incorporation by using response surface methodology under a three-factor(straw length, amount, and burying depth), five-level quadratic orthogonal rotation experimental design. Weight was assigned to each indicator for soil carbon and nutrients and then a comprehensive indicator was established. Then, a second-order polynomial model of the three straw returning factors was established using response surface methodology. Results indicated that17 months after straw incorporation, straw amount and burying depth had significant effects on the comprehensive indicator of soil carbon and nutrients. Straw length and the interactions of straw amount and burying depth showed no significant effects on the comprehensive indicator of soil carbon and nutrients. It was concluded that 17 months after straw incorporation, the highest value of the comprehensive indicator of soil carbon and nutrients was achieved when the straw length, amount, and burying depth were approximately 17–20 cm, 740–840 g m^(-2), and 9–13 cm, respectively, which can be recommended as the most suitable parameters for use in straw returning in the study area. 展开更多
关键词 agricultural management practice soil fertility soil organic carbon straw amount straw burying depth straw length straw returning factors
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