Objective:To identify the antibacterial potential of seagrass(Syringodium isoetifoliutn) associate microbes against bacterial pathogens.Methods:Eumeration of microbial associates were analyzed with leaf and root sampl...Objective:To identify the antibacterial potential of seagrass(Syringodium isoetifoliutn) associate microbes against bacterial pathogens.Methods:Eumeration of microbial associates were analyzed with leaf and root samples of Syringodium isoetifolium.MIC and MBC were calculated for bacterial pathogens with microbial associates.Phylogenetic and GC-MS analysis were calculated for Actinomycetes sp.(Act01) which was the most potent.Results:Of the isolated microbial associates phosphatase producing bacterial isolates were identified as maximum [(26l.78±35.09) CFU×10~4/g]counts in root sample.Of the selected microbial isolates Actinomycete sp(Act01) showed broad spectrum of antibacterial activity against antibiotic resistant and fish bacterial pathogens.Phylogenetic analysis of Act01 showed maximum identities(99%) with the Streptomyces sp.(GU5500072).The 16s rDNA secondary structure of Act01 showed the free energy values as -366.3 kkal/mol.The GC-MS analysis Act01 showed maximum retention value with 23.742 RT and the corresponding chemical class was identified as 1,4-dihydroxy-2-(3- hydroxybutyl)-9,10-anthraquinone 9,10-anthrac.Conclusions:In conclusion,Streptomyces sp. (GU045544.1) from Syringodium isoetifolium could be used as potential antibacterial agent.展开更多
基金the Indian Council of Medical Research(ICMR),New Delhi for financial assistance
文摘Objective:To identify the antibacterial potential of seagrass(Syringodium isoetifoliutn) associate microbes against bacterial pathogens.Methods:Eumeration of microbial associates were analyzed with leaf and root samples of Syringodium isoetifolium.MIC and MBC were calculated for bacterial pathogens with microbial associates.Phylogenetic and GC-MS analysis were calculated for Actinomycetes sp.(Act01) which was the most potent.Results:Of the isolated microbial associates phosphatase producing bacterial isolates were identified as maximum [(26l.78±35.09) CFU×10~4/g]counts in root sample.Of the selected microbial isolates Actinomycete sp(Act01) showed broad spectrum of antibacterial activity against antibiotic resistant and fish bacterial pathogens.Phylogenetic analysis of Act01 showed maximum identities(99%) with the Streptomyces sp.(GU5500072).The 16s rDNA secondary structure of Act01 showed the free energy values as -366.3 kkal/mol.The GC-MS analysis Act01 showed maximum retention value with 23.742 RT and the corresponding chemical class was identified as 1,4-dihydroxy-2-(3- hydroxybutyl)-9,10-anthraquinone 9,10-anthrac.Conclusions:In conclusion,Streptomyces sp. (GU045544.1) from Syringodium isoetifolium could be used as potential antibacterial agent.
