Two-dimensional(2D)Nb_(2)CT_(x)MXene hold great promise for biomedical applications due to their tunable surface chemistry and biocompatibility.However,their practical use requires long-term colloidal and oxidative st...Two-dimensional(2D)Nb_(2)CT_(x)MXene hold great promise for biomedical applications due to their tunable surface chemistry and biocompatibility.However,their practical use requires long-term colloidal and oxidative stability.Here,we propose a tandem-type stabilization strategy combining antioxidant protection and macromolecular surface functionalization.Nb_(2)CT_(x)was first treated with L-ascorbic acid(LA)to suppress oxidation by binding to reactive edges,followed by modification with polyethylene glycol(PEG),poly-L-lysine(PLL),or polydopamine(PDA).This dual approach enhanced stability in biological media—phosphate-buffered saline(PBS)and Dulbecco’s Modified Eagle’s Medium(DMEM)—while preserving non-cytotoxicity toward A375 and HaCaT skin cell lines across 0‒100 mg·L^(-1).Among the tested systems,LA/PEG and LA/PDAmodified MXenes maintained stable zeta potentials(-15 to-12 mV)and particle sizes for 72 h,whereas LA/PLL samples showed aggregation and charge loss.This tandem stabilization effectively prevents oxidation and aggregation without compromising biocompatibility,offering a versatile route for developing oxidation-resistant MXenes for biomedical and nanomedicine applications.展开更多
基金funded by the National Science Centre(NCN)within the framework of the research project‘OPUS-23’(UMO-2022/45/B/ST5/03652)and‘PRELUDIUM 17’(UMO-2019/33/N/ST5/02095)the National Science Centre,within the framework of the research project‘PRELUDIUM-22’(UMO-2023/49/N/ST11/03574)and‘OPUS-18’(UMO-2019/35/B/ST5/02538)+1 种基金the Foundation for Polish Science(FNP,Scholarship START program)ID-UB project(Scholarship Plus program).
文摘Two-dimensional(2D)Nb_(2)CT_(x)MXene hold great promise for biomedical applications due to their tunable surface chemistry and biocompatibility.However,their practical use requires long-term colloidal and oxidative stability.Here,we propose a tandem-type stabilization strategy combining antioxidant protection and macromolecular surface functionalization.Nb_(2)CT_(x)was first treated with L-ascorbic acid(LA)to suppress oxidation by binding to reactive edges,followed by modification with polyethylene glycol(PEG),poly-L-lysine(PLL),or polydopamine(PDA).This dual approach enhanced stability in biological media—phosphate-buffered saline(PBS)and Dulbecco’s Modified Eagle’s Medium(DMEM)—while preserving non-cytotoxicity toward A375 and HaCaT skin cell lines across 0‒100 mg·L^(-1).Among the tested systems,LA/PEG and LA/PDAmodified MXenes maintained stable zeta potentials(-15 to-12 mV)and particle sizes for 72 h,whereas LA/PLL samples showed aggregation and charge loss.This tandem stabilization effectively prevents oxidation and aggregation without compromising biocompatibility,offering a versatile route for developing oxidation-resistant MXenes for biomedical and nanomedicine applications.
