This study aims to investigate the antioxidant potential,bioactive retention,and antidepressant effects of native and encapsulated oleoresins from pepper,turmeric,and chili to enhance their therapeutic efficacy.The ma...This study aims to investigate the antioxidant potential,bioactive retention,and antidepressant effects of native and encapsulated oleoresins from pepper,turmeric,and chili to enhance their therapeutic efficacy.The major challenge in utilizing spice oleoresins is their low bioavailability and stability,which this study addresses through nano-encapsulation using hydroxypropyl beta-cyclodextrin(HpBcD).The encapsulated oleoresins were evaluated for their total polyphenol,flavonoid content,and antioxidant activity,revealing that turmeric oleo resin(TOR)demonstrated the highest antioxidant potential,followed by chili(COR)and pepper(POR).How ever,nano-encapsulation reduced antioxidant activity by 50-60%,although it significantly improved the stability and bioavailability of bioactives.Molecular docking studies further confirmed strong binding affinities between bioactive compounds(piperine,curcumin,and capsaicin)and depression-related proteins,including serotonin,Brain-Derived Neurotrophic Factor(BDNF),and Monoamine Oxidase(MAO),indicating potential antidepressant effects.This study highlights the novelty of nano-encapsulation in enhancing the bioactivity and therapeutic potential of spice oleoresins,offering promising insights for developing natural antidepressant for-mulations or functional foods to combat depression and neurodegenerative disorders.展开更多
Objective:This study investigated the oral bioavailability and anti-obesity effects of oleoresin extracted from seeds of Nigella sativa L.(NS),in high-fat-diet-fed C57BL/6 mice.NS is rich in polyphenols,flavonoids,and...Objective:This study investigated the oral bioavailability and anti-obesity effects of oleoresin extracted from seeds of Nigella sativa L.(NS),in high-fat-diet-fed C57BL/6 mice.NS is rich in polyphenols,flavonoids,and unsaturated fatty acids;known for their anti-oxidant and anti-inflammatory activity.However,its role in modulating lipid and energy metabolism remains unexplored.Methods:Oleoresin was administered orally as well as intravenously(IV),and whole blood was collected at predetermined time points.Thymoquinone(TQ)concentration in plasma was quantified using HPLC.For antiobesity assessment,36 male C57BL/6 mice were divided into six groups,one normal diet and five HFD groups.NS oleoresin was administered daily at doses 50,100,and 200 mg/kg body weight(BW)for 8 weeks.Results:Treatment groups showed significant reductions in body weight,blood glucose,LDL,triglycerides,total cholesterol,and liver size,along with an increase in HDL levels.Gene expression analysis revealed that oleoresin downregulated CCAAT/enhancer-binding protein alpha(C/EBPα)and peroxisome proliferator-activated receptor gamma(PPARγ)expression,while slightly upregulating adenosine monophosphate-activated protein kinase(AMPKα)gene in the higher dose group.Although the mitochondrial uncoupling protein(UCP1)was not significantly altered,expression of sirtuin 1 and PGC-1,was enhanced indicating enhanced mitochondrial biogenesis,insulin sensitivity and weight reduction.Histopathological examination showed reversal of hepatic steatosis,as well as regular-sized adipocytes in dose-dependent treatment.Conclusion:NS seed oleoresin supplementation may reduce the BW and lipid profile induced by HFD when given in higher doses via activation of AMPK/SIRT1 pathway,potentially serving as a functional food therapeutic against obesity and related complications.展开更多
文摘This study aims to investigate the antioxidant potential,bioactive retention,and antidepressant effects of native and encapsulated oleoresins from pepper,turmeric,and chili to enhance their therapeutic efficacy.The major challenge in utilizing spice oleoresins is their low bioavailability and stability,which this study addresses through nano-encapsulation using hydroxypropyl beta-cyclodextrin(HpBcD).The encapsulated oleoresins were evaluated for their total polyphenol,flavonoid content,and antioxidant activity,revealing that turmeric oleo resin(TOR)demonstrated the highest antioxidant potential,followed by chili(COR)and pepper(POR).How ever,nano-encapsulation reduced antioxidant activity by 50-60%,although it significantly improved the stability and bioavailability of bioactives.Molecular docking studies further confirmed strong binding affinities between bioactive compounds(piperine,curcumin,and capsaicin)and depression-related proteins,including serotonin,Brain-Derived Neurotrophic Factor(BDNF),and Monoamine Oxidase(MAO),indicating potential antidepressant effects.This study highlights the novelty of nano-encapsulation in enhancing the bioactivity and therapeutic potential of spice oleoresins,offering promising insights for developing natural antidepressant for-mulations or functional foods to combat depression and neurodegenerative disorders.
文摘Objective:This study investigated the oral bioavailability and anti-obesity effects of oleoresin extracted from seeds of Nigella sativa L.(NS),in high-fat-diet-fed C57BL/6 mice.NS is rich in polyphenols,flavonoids,and unsaturated fatty acids;known for their anti-oxidant and anti-inflammatory activity.However,its role in modulating lipid and energy metabolism remains unexplored.Methods:Oleoresin was administered orally as well as intravenously(IV),and whole blood was collected at predetermined time points.Thymoquinone(TQ)concentration in plasma was quantified using HPLC.For antiobesity assessment,36 male C57BL/6 mice were divided into six groups,one normal diet and five HFD groups.NS oleoresin was administered daily at doses 50,100,and 200 mg/kg body weight(BW)for 8 weeks.Results:Treatment groups showed significant reductions in body weight,blood glucose,LDL,triglycerides,total cholesterol,and liver size,along with an increase in HDL levels.Gene expression analysis revealed that oleoresin downregulated CCAAT/enhancer-binding protein alpha(C/EBPα)and peroxisome proliferator-activated receptor gamma(PPARγ)expression,while slightly upregulating adenosine monophosphate-activated protein kinase(AMPKα)gene in the higher dose group.Although the mitochondrial uncoupling protein(UCP1)was not significantly altered,expression of sirtuin 1 and PGC-1,was enhanced indicating enhanced mitochondrial biogenesis,insulin sensitivity and weight reduction.Histopathological examination showed reversal of hepatic steatosis,as well as regular-sized adipocytes in dose-dependent treatment.Conclusion:NS seed oleoresin supplementation may reduce the BW and lipid profile induced by HFD when given in higher doses via activation of AMPK/SIRT1 pathway,potentially serving as a functional food therapeutic against obesity and related complications.
