The objective of the study was to evaluate the anti-diabetic activities of methanol extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a polyherbal drug (PH) in diabetes induced Wistar albino rats. A single d...The objective of the study was to evaluate the anti-diabetic activities of methanol extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a polyherbal drug (PH) in diabetes induced Wistar albino rats. A single dose of alloxan monohydrate (150 mg/kg, i.p.) was used to induce diabetes mellitus (DM). Diabetes was confirmed by the elevated blood glucose levels determined after 72 h of induction. Animals with mean fasting blood glucose (FBG) level more than 200 mg/dl were recruited for the experiment. The herbal extracts at doses of 200 and 400 mg/kg and standard drugmetformin (5 mg/kg) were administered orally to the diabetic rats for 21 days and the FBG level was estimated on 0, 7, 14 and 21 days. The herbal extracts showed dose-dependent fall in FBG levels and the result exhibited very significant (P < 0.001) decreases in FBG level by the end of the experimental day as compared to the diabetic control. The highest antihyperglycemic effect was observed by MD extract at 400 mg/kg and was comparable to the standard drug. Oral glucose tolerance test (OGTT) was also conducted on normal rats and thus glucose at 2 g/kg per body weight was loaded via oral gavage to all groups 30 min after extract administration. All the groups showed significant increase (P P The hyperglycemia with glucose challenge was significantly brought down (P < 0.001) by all herbal extracts at 60 and 120 min relative to the negative control. Moreover, acute oral toxicity tests was conducted based on the protocols of OECD-425 and thus the LD50 of the herbal extracts was estimated to be greater than 2000 mg/kg. Statistical analysis was performed using One-Way ANOVA followed by Dunnett’s test for multiple comparisons, and values of P < 0.05 were considered as statistically significant.展开更多
Background: Steganotaenia araliacea is a multipurpose plant and has wider applications in the folklore for the treatment of various ailments. Previously the presence of antileukemic lignan lactones and saponins was de...Background: Steganotaenia araliacea is a multipurpose plant and has wider applications in the folklore for the treatment of various ailments. Previously the presence of antileukemic lignan lactones and saponins was detected in the stem bark, root and leaf of Steganotaenia araliacea. Besides, the diuretic and antibacterial activities of the plant were reported. However, there has been no attempt to examine the constituents of the seeds of Steganotaenia araliacea. This paper reports the first such study of both the essential oil and methanol extract of the seeds of the plant. Methods: The seeds of the plant were shade dried, pounded and thus extracted using methanol. Besides, the essential oil of the seeds was collected using steam distillation. The components of the methanol extract were studied both by GC-MS and preliminary phytochemical studies;the essential oil was running on GC-MS for analysis. Results: The GC-MS analysis of the essential oil of the seeds identified the presence of α-linalool, α-pinene, m-cresol, p-menth-1-en-4-ol, p-menth-1-en-8-ol, myristicin and others. Besides, the methanol extract of the seeds showed the presence of falcarinol, apiol, scoparone, stigmasterol, myristicin etc. The preliminary phytochemical analysis of the methanol extract of the seeds confirms the presence of alkaloids, flavonoids, tannins, coumarines, steroids, and phenols. Conclusion: This plant contains bioactive metabolites and thus can be used as an alternative and complementary medicine in treatment of different ailments. However, further studies on the bioactivity and toxicity of the plant should be done.展开更多
文摘The objective of the study was to evaluate the anti-diabetic activities of methanol extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a polyherbal drug (PH) in diabetes induced Wistar albino rats. A single dose of alloxan monohydrate (150 mg/kg, i.p.) was used to induce diabetes mellitus (DM). Diabetes was confirmed by the elevated blood glucose levels determined after 72 h of induction. Animals with mean fasting blood glucose (FBG) level more than 200 mg/dl were recruited for the experiment. The herbal extracts at doses of 200 and 400 mg/kg and standard drugmetformin (5 mg/kg) were administered orally to the diabetic rats for 21 days and the FBG level was estimated on 0, 7, 14 and 21 days. The herbal extracts showed dose-dependent fall in FBG levels and the result exhibited very significant (P < 0.001) decreases in FBG level by the end of the experimental day as compared to the diabetic control. The highest antihyperglycemic effect was observed by MD extract at 400 mg/kg and was comparable to the standard drug. Oral glucose tolerance test (OGTT) was also conducted on normal rats and thus glucose at 2 g/kg per body weight was loaded via oral gavage to all groups 30 min after extract administration. All the groups showed significant increase (P P The hyperglycemia with glucose challenge was significantly brought down (P < 0.001) by all herbal extracts at 60 and 120 min relative to the negative control. Moreover, acute oral toxicity tests was conducted based on the protocols of OECD-425 and thus the LD50 of the herbal extracts was estimated to be greater than 2000 mg/kg. Statistical analysis was performed using One-Way ANOVA followed by Dunnett’s test for multiple comparisons, and values of P < 0.05 were considered as statistically significant.
文摘Background: Steganotaenia araliacea is a multipurpose plant and has wider applications in the folklore for the treatment of various ailments. Previously the presence of antileukemic lignan lactones and saponins was detected in the stem bark, root and leaf of Steganotaenia araliacea. Besides, the diuretic and antibacterial activities of the plant were reported. However, there has been no attempt to examine the constituents of the seeds of Steganotaenia araliacea. This paper reports the first such study of both the essential oil and methanol extract of the seeds of the plant. Methods: The seeds of the plant were shade dried, pounded and thus extracted using methanol. Besides, the essential oil of the seeds was collected using steam distillation. The components of the methanol extract were studied both by GC-MS and preliminary phytochemical studies;the essential oil was running on GC-MS for analysis. Results: The GC-MS analysis of the essential oil of the seeds identified the presence of α-linalool, α-pinene, m-cresol, p-menth-1-en-4-ol, p-menth-1-en-8-ol, myristicin and others. Besides, the methanol extract of the seeds showed the presence of falcarinol, apiol, scoparone, stigmasterol, myristicin etc. The preliminary phytochemical analysis of the methanol extract of the seeds confirms the presence of alkaloids, flavonoids, tannins, coumarines, steroids, and phenols. Conclusion: This plant contains bioactive metabolites and thus can be used as an alternative and complementary medicine in treatment of different ailments. However, further studies on the bioactivity and toxicity of the plant should be done.
