Objective:To investigate the hepatoprotective activity of red algae Portieria hornemannii(Lyngbye)Silva(P.hornemannii)and Spyridia fusiformis Boergesen(S.fusiformis)by using the chromium treated rat liver as the anima...Objective:To investigate the hepatoprotective activity of red algae Portieria hornemannii(Lyngbye)Silva(P.hornemannii)and Spyridia fusiformis Boergesen(S.fusiformis)by using the chromium treated rat liver as the animal model.Methods:The extract of red algae at a dosage of 0.200 g/kg of whole body weight was orally administrated to Cr(VI)intoxicated rats for 28 consecutive days.The effect of drug in rats was evaluated by comparing the degree of the production of enzymes responsible for antioxidant activity such lipid peroxidase,superoxide dismutase,catalase and reduced glutathione with Cr(VI)analogs in the absence of any secondary treatment.The overall damage of liver was detected by measuring serum enzymes such as aspartate amino transferase and alanine aminotransferase activities which released into the blood from the damaged cells.Results:It was observed that these enzyme levels were noticed in the animals treated with methanol extracts of red algae(200 mg/kg)through preventing the leakage of the above enzymes into the blood.The hepatoprotection obtained using LIV 52(standard reference drug)appeared relatively higher.The antihepatotoxic potential of red algae P.hornemannii and S.fusiformis might be due to their antioxidative and membrane stabilizing activities.Conclusions:Our results indicated that the extract of P.hornemannii and S.fusiformis obtained from methanol could be a promising hepatoprotective agent against chromium(VI)-induced liver damage.展开更多
Objective:To study the in vitro cytotoxic activities of methanol extract of Portieria hornemannii(P.hornemannii)and Spyridia fusiformis(S.fusiformis)using Dalton’s lymphoma ascite and Ehrlich ascite carcinoma cell li...Objective:To study the in vitro cytotoxic activities of methanol extract of Portieria hornemannii(P.hornemannii)and Spyridia fusiformis(S.fusiformis)using Dalton’s lymphoma ascite and Ehrlich ascite carcinoma cell lines.Methods:The effect of cytotoxicity of P.hornemannii and S.fusiformis was evaluated with the concentrations(100 to 200μg/mL)and assessed for the antitumour activity vs.the selected cell lines using Trypan blue assay.Results:The methanol extracts of P.hornemannii and S.fusiformis showed potent cytotoxic activity with IC_(50)values of(209.00±0.05)μg/mL and(190.00±0.05)μg/mL against the Dalton’s lymphoma ascite cell line and IC_(50)values of(190.00±0.05)μg/mL and(182.00±0.05)μg/mL against the Ehrlich ascite carcinoma cell line respectively.In vitro cytotoxicity against the tested cancer cell lines showed strong activity by the abnormal activities of algal residue in the normal cells.Conclusions:The methanol solvent residue of red algae(P.hornemannii and S.fusiformis)could be a good candidate.It would be a novel marine resource as a antitumor medicine demonstrated by cytotoxic studies that the above marine algae can be a potential candidate sources as antitumor drugs.展开更多
基金Supported by University Grants Commission,New Delhi[Grant No.F1-17.1/2011-12/RGNF-SC-TAM-5342/(SA-III/Website)].
文摘Objective:To investigate the hepatoprotective activity of red algae Portieria hornemannii(Lyngbye)Silva(P.hornemannii)and Spyridia fusiformis Boergesen(S.fusiformis)by using the chromium treated rat liver as the animal model.Methods:The extract of red algae at a dosage of 0.200 g/kg of whole body weight was orally administrated to Cr(VI)intoxicated rats for 28 consecutive days.The effect of drug in rats was evaluated by comparing the degree of the production of enzymes responsible for antioxidant activity such lipid peroxidase,superoxide dismutase,catalase and reduced glutathione with Cr(VI)analogs in the absence of any secondary treatment.The overall damage of liver was detected by measuring serum enzymes such as aspartate amino transferase and alanine aminotransferase activities which released into the blood from the damaged cells.Results:It was observed that these enzyme levels were noticed in the animals treated with methanol extracts of red algae(200 mg/kg)through preventing the leakage of the above enzymes into the blood.The hepatoprotection obtained using LIV 52(standard reference drug)appeared relatively higher.The antihepatotoxic potential of red algae P.hornemannii and S.fusiformis might be due to their antioxidative and membrane stabilizing activities.Conclusions:Our results indicated that the extract of P.hornemannii and S.fusiformis obtained from methanol could be a promising hepatoprotective agent against chromium(VI)-induced liver damage.
基金Supported by University Grants Commission,New Delhi(Grant No.F1-17.1/2011-12/RGNF-SC-TAM-5342/(SA-III/Website)).
文摘Objective:To study the in vitro cytotoxic activities of methanol extract of Portieria hornemannii(P.hornemannii)and Spyridia fusiformis(S.fusiformis)using Dalton’s lymphoma ascite and Ehrlich ascite carcinoma cell lines.Methods:The effect of cytotoxicity of P.hornemannii and S.fusiformis was evaluated with the concentrations(100 to 200μg/mL)and assessed for the antitumour activity vs.the selected cell lines using Trypan blue assay.Results:The methanol extracts of P.hornemannii and S.fusiformis showed potent cytotoxic activity with IC_(50)values of(209.00±0.05)μg/mL and(190.00±0.05)μg/mL against the Dalton’s lymphoma ascite cell line and IC_(50)values of(190.00±0.05)μg/mL and(182.00±0.05)μg/mL against the Ehrlich ascite carcinoma cell line respectively.In vitro cytotoxicity against the tested cancer cell lines showed strong activity by the abnormal activities of algal residue in the normal cells.Conclusions:The methanol solvent residue of red algae(P.hornemannii and S.fusiformis)could be a good candidate.It would be a novel marine resource as a antitumor medicine demonstrated by cytotoxic studies that the above marine algae can be a potential candidate sources as antitumor drugs.
