Disorders of the musculoskeletal system are the major contributors to the global burden of disease and current treatments show limited efficacy.Patients often suffer chronic pain and might eventually have to undergo e...Disorders of the musculoskeletal system are the major contributors to the global burden of disease and current treatments show limited efficacy.Patients often suffer chronic pain and might eventually have to undergo end-stage surgery.Therefore,future treatments should focus on early detection and intervention of regional lesions.Microrobots have been gradually used in organisms due to their advantages of intelligent,precise and minimally invasive targeted delivery.Through the combination of control and imaging systems,microrobots with good biosafety can be delivered to the desired area for treatment.In the musculoskeletal system,microrobots are mainly utilized to transport stem cells/drugs or to remove hazardous substances from the body.Compared to traditional biomaterial and tissue engineering strategies,active motion improves the efficiency and penetration of local targeting of cells/drugs.This review discusses the frontier applications of microrobotic systems in different tissues of the musculoskeletal system.We summarize the challenges and barriers that hinder clinical translation by evaluating the characteristics of different microrobots and finally point out the future direction of microrobots in the musculoskeletal system.展开更多
Tendon repair remains challenging due to its poor intrinsic healing capacity,and stem cell therapy has emerged as a promising strategy to promote tendon regeneration.Nevertheless,the inflammatory environment following...Tendon repair remains challenging due to its poor intrinsic healing capacity,and stem cell therapy has emerged as a promising strategy to promote tendon regeneration.Nevertheless,the inflammatory environment following acute tendon injuries disrupts stem cell differentiation,leading to unsatisfied outcomes.Our study recognized the critical role of NF-κB signaling in activating inflammation and suppressing tenogenic differentiation of stem cells after acute tendon injury via multiomics analysis.TPCA-1,a selective inhibitor of IKKβ/NF-κB signaling,effi-ciently restored the impaired tenogenesis of stem cells in the inflammatory environment.By developing a microsphere-incorporated hydrogel system for stem cell delivery and controlled release of TPCA-1,we suc-cessfully engineered a pro-tenogenic niche to initiate tenogenesis for tendon regeneration.Collectively,we recognize NF-κB signaling as a critical target to tailor a pro-tenogenic niche and propose the combined delivery of stem cells and TPCA-1 as a potential strategy for acute tendon injuries.展开更多
基金supported by the National Natural Science Foundation of China(No.81572187,No.81871812 and No.52205590)the Natural Science Foundation of Jiangsu Province(No.BK20220834)+1 种基金project supported by Ruihua Charity Foundation(YL20220525)the Start-up Research Fund of Southeast University(No.RF1028623098).
文摘Disorders of the musculoskeletal system are the major contributors to the global burden of disease and current treatments show limited efficacy.Patients often suffer chronic pain and might eventually have to undergo end-stage surgery.Therefore,future treatments should focus on early detection and intervention of regional lesions.Microrobots have been gradually used in organisms due to their advantages of intelligent,precise and minimally invasive targeted delivery.Through the combination of control and imaging systems,microrobots with good biosafety can be delivered to the desired area for treatment.In the musculoskeletal system,microrobots are mainly utilized to transport stem cells/drugs or to remove hazardous substances from the body.Compared to traditional biomaterial and tissue engineering strategies,active motion improves the efficiency and penetration of local targeting of cells/drugs.This review discusses the frontier applications of microrobotic systems in different tissues of the musculoskeletal system.We summarize the challenges and barriers that hinder clinical translation by evaluating the characteristics of different microrobots and finally point out the future direction of microrobots in the musculoskeletal system.
基金financially supported by the National Key Research and Development Program of China(2023YFE0206700)the National Natural Science Foundation of China(82372139)+3 种基金the Natural Science Foundation of Jiangsu Province(BK20242030,BK20240176)Shandong Provincial key research and development program(2021ZDSYS14)the Fundamental Research Funds for the Central Universities(2242024K40040,2242023K40034)the National Undergraduate Training Programs for Innovation and Entrepreneurship(202410286144Z)。
文摘Tendon repair remains challenging due to its poor intrinsic healing capacity,and stem cell therapy has emerged as a promising strategy to promote tendon regeneration.Nevertheless,the inflammatory environment following acute tendon injuries disrupts stem cell differentiation,leading to unsatisfied outcomes.Our study recognized the critical role of NF-κB signaling in activating inflammation and suppressing tenogenic differentiation of stem cells after acute tendon injury via multiomics analysis.TPCA-1,a selective inhibitor of IKKβ/NF-κB signaling,effi-ciently restored the impaired tenogenesis of stem cells in the inflammatory environment.By developing a microsphere-incorporated hydrogel system for stem cell delivery and controlled release of TPCA-1,we suc-cessfully engineered a pro-tenogenic niche to initiate tenogenesis for tendon regeneration.Collectively,we recognize NF-κB signaling as a critical target to tailor a pro-tenogenic niche and propose the combined delivery of stem cells and TPCA-1 as a potential strategy for acute tendon injuries.
