Lacustrine groundwater discharge(LGD)plays an important role in water resources management.Previous studies have focused on LGD process in a single lake,but the differences in LGD process within the same region have n...Lacustrine groundwater discharge(LGD)plays an important role in water resources management.Previous studies have focused on LGD process in a single lake,but the differences in LGD process within the same region have not been thoroughly investigated.In this study,multiple tracers(hydrochemistry,𝛿D,𝛿18O and 222Rn)were used to compare mechanisms of LGD in Daihai and Ulansuhai Lake in Inner Mongoli1,Northwest China.The hydrochemical types showed a trend from groundwater to lake water,indicating a hydraulic connection between them.In addition,the𝛿D and𝛿18O values of sediment pore water were between the groundwater and lake water,indicating the LGD processes.The radon mass balance model was used to estimate the average groundwater discharge rates of Daihai and Ulansuhai Lake,which were 2.79 mm/day and 3.02 mm/day,respectively.The total nitrogen(TN),total phosphorus(TP),and fluoride inputs associated with LGD in Daihai Lake accounted for 97.52%,96.59%,and 95.84%of the total inputs,respectively.In contrast,TN,TP and fluoride inputs in Ulansuhai Lake were 53.56%,40.98%,and 36.25%,respectively.This indicates that the pollutant inputs associated with LGD posed a potential threat to the ecological stability of Daihai and Ulansuhai Lake.By comparison,the differences of LGD process and associated pollutant flux were controlled by hydrogeological conditions,lakebed permeability and human activities.This study provides a reference for water resources management in Daihai and Ulansuhai Lake basins while improving the understanding of LGD in the Yellow River basin.展开更多
Disruption of acylcarnitine homeostasis results in life-threatening outcomes in humans.Carnitine-acylcarnitine translocase deficiency(CACTD)is a scarce autosomal recessive genetic disease and may result in patients’d...Disruption of acylcarnitine homeostasis results in life-threatening outcomes in humans.Carnitine-acylcarnitine translocase deficiency(CACTD)is a scarce autosomal recessive genetic disease and may result in patients’death due to heart arrest or respiratory insufficiency.However,the reasons and mechanism of CACTD inducing respiratory insufficiency have never been elucidated.Herein,we employed lipidomic techniques to create comprehensive lipidomic maps of entire lungs throughout both prenatal and postnatal developmental stages in mice.We found that the acylcarnitines manifested notable variations and coordinated the expression levels of carnitine-acylcarnitine translocase(Cact)across these lung developmental stages.Cact-null mice were all dead with a symptom of respiratory distress and exhibited failed lung development.Loss of Cact resulted in an accumulation of palmitoyl-carnitine(C16-acylcarnitine)in the lungs and promoted the proliferation of mesenchymal progenitor cells.Mesenchymal cells with elevated C16-acylcarnitine levels displayed minimal changes in energy metabolism but,upon investigation,revealed an interaction with sterile alpha motif domain and histidine-aspartate domain-containing protein 1(Samhd1),leading to decreased protein abundance and enhanced cell proliferation.Thus,our findings present a mechanism addressing respiratory distress in CACTD,offering a valuable reference point for both the elucidation of pathogenesis and the exploration of treatment strategies for neonatal respiratory distress.展开更多
Wild bees are ecologically vital but increasingly threatened by anthropogenic activities,leading to uncertain survival and health outcomes in urban environments.The gut microbiome contains features indicating host hea...Wild bees are ecologically vital but increasingly threatened by anthropogenic activities,leading to uncertain survival and health outcomes in urban environments.The gut microbiome contains features indicating host health and reflecting long-term evolutionary adaptation and acute reactions to real-time stressors.Moving beyond bacteria,we propose a comprehensive analysis integrating diet,bacteriome,virome,resistome,and their association to understand the survival status of urban lives better.We conducted a study on mason bees(Osmia excavata)across 10 urban agricultural sites in Suzhou,China,using shotgun gut metagenome sequencing for data derived from total gut DNA.Our findings revealed that most ingested pollen originated from Brassica crops and the unexpected garden tree Plantanus,indicating that floral resources at the 10 sites supported Osmia but with limited plant diversity.Varied city landscapes revealed site-specific flowers that all contributed to Osmia sustenance.The gut bacterial community,dominated by Gammaproteobacteria,showed remarkable structural stability across 8 sites but suggested perturbations at 2 sites.Antibiotic resistance gene profiles highly varied across 10 sites with prevalent unclassified drug classes,highlighting environmental threats to both bees and humans.The virome analysis identified honeybee pathogens,suggesting potential virus spillover.Many unknown bacteriophages were detected,some of which targeted the core gut bacteria,underscoring their role in maintaining gut homeostasis.These multifaceted metagenomic insights hold the potential to predict bee health and identify environmental threats,thereby guiding probiotic development and city management for effective bee conservation.展开更多
基金supported by the Natural Science Foundation of Inner Mongolia Autonomous Region of China(No.2023QN04011)the National Natural Science Foundation of China(Nos.42307092 and 52279067)+1 种基金Ordos Science and Technology Major Project(No.ZD20232303)Project of Key Laboratory of River and Lake in Inner Mongolia Autonomous Region(No.2022QZBZ0003).
文摘Lacustrine groundwater discharge(LGD)plays an important role in water resources management.Previous studies have focused on LGD process in a single lake,but the differences in LGD process within the same region have not been thoroughly investigated.In this study,multiple tracers(hydrochemistry,𝛿D,𝛿18O and 222Rn)were used to compare mechanisms of LGD in Daihai and Ulansuhai Lake in Inner Mongoli1,Northwest China.The hydrochemical types showed a trend from groundwater to lake water,indicating a hydraulic connection between them.In addition,the𝛿D and𝛿18O values of sediment pore water were between the groundwater and lake water,indicating the LGD processes.The radon mass balance model was used to estimate the average groundwater discharge rates of Daihai and Ulansuhai Lake,which were 2.79 mm/day and 3.02 mm/day,respectively.The total nitrogen(TN),total phosphorus(TP),and fluoride inputs associated with LGD in Daihai Lake accounted for 97.52%,96.59%,and 95.84%of the total inputs,respectively.In contrast,TN,TP and fluoride inputs in Ulansuhai Lake were 53.56%,40.98%,and 36.25%,respectively.This indicates that the pollutant inputs associated with LGD posed a potential threat to the ecological stability of Daihai and Ulansuhai Lake.By comparison,the differences of LGD process and associated pollutant flux were controlled by hydrogeological conditions,lakebed permeability and human activities.This study provides a reference for water resources management in Daihai and Ulansuhai Lake basins while improving the understanding of LGD in the Yellow River basin.
基金supported by the National Key R&D Program of China(2022YFC3600202 and 2018YFA0800902)the National Natural Science Foundation of China(32170843)the Major Project of Guangzhou National Laboratory(Grant No.GZNL2024A03013).
文摘Disruption of acylcarnitine homeostasis results in life-threatening outcomes in humans.Carnitine-acylcarnitine translocase deficiency(CACTD)is a scarce autosomal recessive genetic disease and may result in patients’death due to heart arrest or respiratory insufficiency.However,the reasons and mechanism of CACTD inducing respiratory insufficiency have never been elucidated.Herein,we employed lipidomic techniques to create comprehensive lipidomic maps of entire lungs throughout both prenatal and postnatal developmental stages in mice.We found that the acylcarnitines manifested notable variations and coordinated the expression levels of carnitine-acylcarnitine translocase(Cact)across these lung developmental stages.Cact-null mice were all dead with a symptom of respiratory distress and exhibited failed lung development.Loss of Cact resulted in an accumulation of palmitoyl-carnitine(C16-acylcarnitine)in the lungs and promoted the proliferation of mesenchymal progenitor cells.Mesenchymal cells with elevated C16-acylcarnitine levels displayed minimal changes in energy metabolism but,upon investigation,revealed an interaction with sterile alpha motif domain and histidine-aspartate domain-containing protein 1(Samhd1),leading to decreased protein abundance and enhanced cell proliferation.Thus,our findings present a mechanism addressing respiratory distress in CACTD,offering a valuable reference point for both the elucidation of pathogenesis and the exploration of treatment strategies for neonatal respiratory distress.
基金supported by the Research Development Fund(RDF-23-01-067)the Summer Undergraduate Research Fellowship(SURF)Program(SURF-2023-0079)from Xi'an Jiaotong-Liverpool University to M.T.
文摘Wild bees are ecologically vital but increasingly threatened by anthropogenic activities,leading to uncertain survival and health outcomes in urban environments.The gut microbiome contains features indicating host health and reflecting long-term evolutionary adaptation and acute reactions to real-time stressors.Moving beyond bacteria,we propose a comprehensive analysis integrating diet,bacteriome,virome,resistome,and their association to understand the survival status of urban lives better.We conducted a study on mason bees(Osmia excavata)across 10 urban agricultural sites in Suzhou,China,using shotgun gut metagenome sequencing for data derived from total gut DNA.Our findings revealed that most ingested pollen originated from Brassica crops and the unexpected garden tree Plantanus,indicating that floral resources at the 10 sites supported Osmia but with limited plant diversity.Varied city landscapes revealed site-specific flowers that all contributed to Osmia sustenance.The gut bacterial community,dominated by Gammaproteobacteria,showed remarkable structural stability across 8 sites but suggested perturbations at 2 sites.Antibiotic resistance gene profiles highly varied across 10 sites with prevalent unclassified drug classes,highlighting environmental threats to both bees and humans.The virome analysis identified honeybee pathogens,suggesting potential virus spillover.Many unknown bacteriophages were detected,some of which targeted the core gut bacteria,underscoring their role in maintaining gut homeostasis.These multifaceted metagenomic insights hold the potential to predict bee health and identify environmental threats,thereby guiding probiotic development and city management for effective bee conservation.
