For the development of 19-plex Y STR system and polymorphism studies in local ethnic populations sixteen markers of non-recombining regions (NRY) of Y chromosome, which show high power of discrimination among individu...For the development of 19-plex Y STR system and polymorphism studies in local ethnic populations sixteen markers of non-recombining regions (NRY) of Y chromosome, which show high power of discrimination among individuals, were selected in this study. Blood samples (600) were collected from the males of three most common castes of Pakistani population (Arain, Awan and Rajput) with different parent lineages. Three markers (DYS385a/b, DYS389I/II and YCAIIa/b) among 16 Y STRs are double-targeted regions of the Y chromosome and thus provide two polymorphic peaks for each respective primer set. These 16 Y-STRs were developed into Megaplex system for simultaneous amplification of all markers within the population. The overall power of discrimination observed in focused populations was 60.5%, 66.5% and 55% in Rajput, Awan and Arain casts respectively. This discrimination power will be helpful in human identification for forensic casework studies including sexual assaults and paternity testing.展开更多
Studies have reported on the economic impacts of clinical and subclinical mastitis on dairy farms.Bovine mastitis is a disorder that affects dairy farms and has a major economic impact.Most of the economic losses are ...Studies have reported on the economic impacts of clinical and subclinical mastitis on dairy farms.Bovine mastitis is a disorder that affects dairy farms and has a major economic impact.Most of the economic losses are the result of mastitis.Mastitis is an invasive infection that is among the most numerous and highly complicated infections in the dairy sector.Mastitis is one of the most expensive diseases in terms of production losses among animal diseases.Mastitis reduces milk production,changes milk composition,and shortens the productive life of infected cows.Farmers must concentrate on avoiding mastitis infection whilst putting in place and following a mastitis control programed.Bovine mastitis,the most significant disease of dairy herds,has huge effects on farm economics.Mastitis losses are due to reduced milk production,the cost of treatments,and culling.Major factors related to low milk yield could be low genetic potential as well as poor nutritional and managerial approaches.Most of the losses are related to somatic cell count(SCC),which is characterised by an increase in the percentage of milk.Culling costs are the costs of rearing or buying a replacement animal,mostly heifers.Overhead impacts include the replacement animals'lower milk supply effectiveness.The expense of replacing animals prematurely due to mastitis is one of the most significant areas of economic loss.展开更多
文摘For the development of 19-plex Y STR system and polymorphism studies in local ethnic populations sixteen markers of non-recombining regions (NRY) of Y chromosome, which show high power of discrimination among individuals, were selected in this study. Blood samples (600) were collected from the males of three most common castes of Pakistani population (Arain, Awan and Rajput) with different parent lineages. Three markers (DYS385a/b, DYS389I/II and YCAIIa/b) among 16 Y STRs are double-targeted regions of the Y chromosome and thus provide two polymorphic peaks for each respective primer set. These 16 Y-STRs were developed into Megaplex system for simultaneous amplification of all markers within the population. The overall power of discrimination observed in focused populations was 60.5%, 66.5% and 55% in Rajput, Awan and Arain casts respectively. This discrimination power will be helpful in human identification for forensic casework studies including sexual assaults and paternity testing.
文摘Studies have reported on the economic impacts of clinical and subclinical mastitis on dairy farms.Bovine mastitis is a disorder that affects dairy farms and has a major economic impact.Most of the economic losses are the result of mastitis.Mastitis is an invasive infection that is among the most numerous and highly complicated infections in the dairy sector.Mastitis is one of the most expensive diseases in terms of production losses among animal diseases.Mastitis reduces milk production,changes milk composition,and shortens the productive life of infected cows.Farmers must concentrate on avoiding mastitis infection whilst putting in place and following a mastitis control programed.Bovine mastitis,the most significant disease of dairy herds,has huge effects on farm economics.Mastitis losses are due to reduced milk production,the cost of treatments,and culling.Major factors related to low milk yield could be low genetic potential as well as poor nutritional and managerial approaches.Most of the losses are related to somatic cell count(SCC),which is characterised by an increase in the percentage of milk.Culling costs are the costs of rearing or buying a replacement animal,mostly heifers.Overhead impacts include the replacement animals'lower milk supply effectiveness.The expense of replacing animals prematurely due to mastitis is one of the most significant areas of economic loss.
