In the present study,we aimed to investigate a protective role for resveratrol against the effects of immobilization stress on corpora lutea(CL)of mice in early pregnancy.A total of 45 early-pregnant mice were divided...In the present study,we aimed to investigate a protective role for resveratrol against the effects of immobilization stress on corpora lutea(CL)of mice in early pregnancy.A total of 45 early-pregnant mice were divided into no immobilization stress(NIS)group,immobilization stress(IS)group,and immobilization and resveratrol treatment(IS+RES)group(n=15).Mice were immobilized in plastic tubes(50 mL)for 3 h per day during day 1 to 7 of pregnancy.In the IS+RES group,5 mg kg-'d-1 of resveratrol was administered just prior to application of stress.We analyzed apoptotic activity in CL by Western botting analysis(WB),transmission electron microscopy(TEM),and immunohistochemistry(IHC).Serum progesterone levels were examined with radioimmunoassay(RIA).IHC results showed that the intensity of positive staining for Bax was increased,and for BcI-2 was decreased in CL after IS,while resveratrol treatment reversed the positive staining for Bax and Bcl-2.WB revealed that immobilization stress up-regulated the expression of Bax and caspase-9,and down-regulated Bcl-2 expression,while resveratrol treatment attenuated the effects of immobilization stress on the expression of Bax,Bcl-2 and caspase-9.According to our TEM results,apoptosis as defined by chromatin condensation was found in CL after immobilization stress,while resveratrol inhibited the apoptosis.We also demonstrated that immobilization stress decreased progesterone concentrations and ovarian expression of StAR,while resveratrol restored the concentrations of progesterone and expression of StAR back to normal.These results indicated that immobilization stress induced luteal regression while resveratrol inhibited luteal regression,suggesting that resveratrol plays a protective role on corpora lutea of mice during early pregnancy.展开更多
Abiotic stresses are among the major limiting factors for plant growth and crop productivity.Among these,salinity is one of the major risk factors for plant growth and development in arid to semi-arid regions.Cultivat...Abiotic stresses are among the major limiting factors for plant growth and crop productivity.Among these,salinity is one of the major risk factors for plant growth and development in arid to semi-arid regions.Cultivation of salt tolerant crop genotypes is one of the imperative approaches to meet the food demand for increasing population.The current experiment was carried out to access the performance of different rice genotypes under salinity stress and Zinc(Zn)sources.Four rice genotypes were grown in a pot experiment and were exposed to salinity stress(7 dS m^(−1)),and Zn(15 mg kg^(-1)soil)was applied from two sources,ZnSO4 and Zn-EDTA.A control of both salinity and Zn was kept for comparison.Results showed that based on the biomass accumulation and K^(+)/Na^(+)ratio,KSK-133 and BAS-198 emerged as salt tolerant and salt sensitive,respectively.Similarly,based on the Zn concentration,BAS-2000 was reported as Zn-in-efficient while IR-6 was a Zn-efficient genotype.Our results also revealed that plant growth,relative water content(RWC),physiological attributes including chlorophyll contents,ionic concentrations in straw and grains of all rice genotypes were decreased under salinity stress.However,salt tolerant and Zn-in-efficient rice genotypes showed significantly higher shoot K^(+)and Zn concentrations under saline conditions.Zinc application significantly alleviates the harmful effects of salinity by improving morpho-physiological attributes and enhancing antioxidant enzyme activities,and the uptake of K and Zn.The beneficial effect of Zn was more pronounced in salt-tolerant and Zn in-efficient rice genotypes as compared with salt-sensitive and Zn-efficient genotypes.In sum,our results confirmed that Zn application increased overall plant’s performance under saline conditions,particularly in Zn in-efficient and tolerant genotypes as compared with salt-sensitive and Zn efficient rice genotypes.展开更多
基金The authors wish to thank Prof.Emeritus Reinhold J.Hutz,PhD of the Department of Biological Sciences,University of Wisconsin-Milwaukee,USA,for his editing and helpful adviceThis work was supported by the National Natural Science Foundation of China(31501956 and 31572403).
文摘In the present study,we aimed to investigate a protective role for resveratrol against the effects of immobilization stress on corpora lutea(CL)of mice in early pregnancy.A total of 45 early-pregnant mice were divided into no immobilization stress(NIS)group,immobilization stress(IS)group,and immobilization and resveratrol treatment(IS+RES)group(n=15).Mice were immobilized in plastic tubes(50 mL)for 3 h per day during day 1 to 7 of pregnancy.In the IS+RES group,5 mg kg-'d-1 of resveratrol was administered just prior to application of stress.We analyzed apoptotic activity in CL by Western botting analysis(WB),transmission electron microscopy(TEM),and immunohistochemistry(IHC).Serum progesterone levels were examined with radioimmunoassay(RIA).IHC results showed that the intensity of positive staining for Bax was increased,and for BcI-2 was decreased in CL after IS,while resveratrol treatment reversed the positive staining for Bax and Bcl-2.WB revealed that immobilization stress up-regulated the expression of Bax and caspase-9,and down-regulated Bcl-2 expression,while resveratrol treatment attenuated the effects of immobilization stress on the expression of Bax,Bcl-2 and caspase-9.According to our TEM results,apoptosis as defined by chromatin condensation was found in CL after immobilization stress,while resveratrol inhibited the apoptosis.We also demonstrated that immobilization stress decreased progesterone concentrations and ovarian expression of StAR,while resveratrol restored the concentrations of progesterone and expression of StAR back to normal.These results indicated that immobilization stress induced luteal regression while resveratrol inhibited luteal regression,suggesting that resveratrol plays a protective role on corpora lutea of mice during early pregnancy.
基金This research was funded by Princess Nourah bint Abdulrahman University,Researchers Supporting Project Number(PNURSP2023R188),Riyadh,Saudi Arabia.
文摘Abiotic stresses are among the major limiting factors for plant growth and crop productivity.Among these,salinity is one of the major risk factors for plant growth and development in arid to semi-arid regions.Cultivation of salt tolerant crop genotypes is one of the imperative approaches to meet the food demand for increasing population.The current experiment was carried out to access the performance of different rice genotypes under salinity stress and Zinc(Zn)sources.Four rice genotypes were grown in a pot experiment and were exposed to salinity stress(7 dS m^(−1)),and Zn(15 mg kg^(-1)soil)was applied from two sources,ZnSO4 and Zn-EDTA.A control of both salinity and Zn was kept for comparison.Results showed that based on the biomass accumulation and K^(+)/Na^(+)ratio,KSK-133 and BAS-198 emerged as salt tolerant and salt sensitive,respectively.Similarly,based on the Zn concentration,BAS-2000 was reported as Zn-in-efficient while IR-6 was a Zn-efficient genotype.Our results also revealed that plant growth,relative water content(RWC),physiological attributes including chlorophyll contents,ionic concentrations in straw and grains of all rice genotypes were decreased under salinity stress.However,salt tolerant and Zn-in-efficient rice genotypes showed significantly higher shoot K^(+)and Zn concentrations under saline conditions.Zinc application significantly alleviates the harmful effects of salinity by improving morpho-physiological attributes and enhancing antioxidant enzyme activities,and the uptake of K and Zn.The beneficial effect of Zn was more pronounced in salt-tolerant and Zn in-efficient rice genotypes as compared with salt-sensitive and Zn-efficient genotypes.In sum,our results confirmed that Zn application increased overall plant’s performance under saline conditions,particularly in Zn in-efficient and tolerant genotypes as compared with salt-sensitive and Zn efficient rice genotypes.
