Sago palm is flowering and fruiting just once in their life cycle. Sago palms that grow naturally and semi cultivated were generally occurred natural pollination to form fruits and seeds, if not cut down to take the s...Sago palm is flowering and fruiting just once in their life cycle. Sago palms that grow naturally and semi cultivated were generally occurred natural pollination to form fruits and seeds, if not cut down to take the starch contained in their trunk. Sago palm pollination may occur as self-pollinated and cross-pollinated. If cross-pollinated was occurred in the pollination process, it will be varied of their progenies. This study aims to reveal the genetic variation of sago palms progenies with naturally pollinated process. The research method is to collect seeds from one parent trees that have produced ripe fruit. Fruit seeds germinated to be made and tested genetic variation using RAPD markers. Isolation of DNA is done by using the fresh young leaves. DNA amplification is done by using RAPD primers. The results showed that the progenies derived from naturally pollinated of sago palms were genetically varied based on RAPD markers and also varied based on morphological phenotypic. Variations occurred in the progenies of sago palm indicated that the sago palms were estimated cross-pollinated naturally, as a result fruits and seeds with genetically differences.展开更多
Orchidaceae has known as an attractive flower and immense species. We have found a large species of Orchidaceae grow naturally in Papua’s jungle, Indonesia territorial. This study aims to reveal genetic variation and...Orchidaceae has known as an attractive flower and immense species. We have found a large species of Orchidaceae grow naturally in Papua’s jungle, Indonesia territorial. This study aims to reveal genetic variation and genetic relationships among endemic orchids in Papua based on RAPD markers. The study included 26 accessions of Papua’s endemic orchids used for genomic DNA extraction. Genomic DNAs were extracted by usingDNAextraction kit from Qiagen and genomic DNA amplification by using 10 decamer RAPD primers. DNA fragments that were amplified by Polymerase Change Reaction (PCR) were visualized and documented by using UV illumination apparatus. Genetically, endemic Orchids in Papua were described high variation. Fragments amplification by using ten RAPD primers and performed in the PCR tools resulted in 54 numbers of polymorphic fragments and no monomorphic band. The number of polymorphic bands per primer ranged from 4 to 7 with averaged 5.4 bands per assay unit. The genetic dissimilarities (GDs) among examined orchids ranged from 0.10 to 0.94 based on Nei’s unbiased coefficients. Dendrogram construction showed that Papua’s endemic orchid (PEO) samples different from another and separated to form group by their own at the 0.40 coefficient value and at the 0.6 coefficient value indicate that PEO sample is divided into nine groups i.e. samples at the genera level were separated into their own groups.展开更多
Papua is rich in biodiversity. The indigenous Papuans utilize most of endemic plants as food, board and medicines. One of the plants used as medicine is La-portea, sp, that locally known as Daun Gatal. The plant howev...Papua is rich in biodiversity. The indigenous Papuans utilize most of endemic plants as food, board and medicines. One of the plants used as medicine is La-portea, sp, that locally known as Daun Gatal. The plant however, has not been developed commercially;this might due to lack of scientific providence of medicine content in this plant. The aims of this research is to (1) explore the types of Laportea growing in Papua, (2) conduct bio molecular character, which observes phylogeny relationship or genetic morphology in order to obtain data on the number of species found in Manokwari and Nabire. Bio molecular analysis is performed by PCR. Genetic analysis in this study was conducted through several phases, as follow: (a) Isolation of genomic total, (b) amplification of genes that encoded Rbcl, (c) sequencing the nucleotide sequence of the gene Rbcl of Laportea, (d) phylogeny analysis. The results showed that the DNA quality was good enough to do the next analysis. Of the four samples, two obtained haplo types are Haplo Type 1 (DT.NBX.H, DT.NBX.U and DR.MKW.H) and Haplo Type 2 (DR.NBX.L). The results of the analysis of phylogeny trees showed samples at 1 in the same haplo type, clade sequences of the genus Dendrocnide spp, Discocnide, and Laportea, while the sample at 2 in the same haplotype clade with Laportea interupta, Laportea ruderalis and Urera sp.展开更多
文摘Sago palm is flowering and fruiting just once in their life cycle. Sago palms that grow naturally and semi cultivated were generally occurred natural pollination to form fruits and seeds, if not cut down to take the starch contained in their trunk. Sago palm pollination may occur as self-pollinated and cross-pollinated. If cross-pollinated was occurred in the pollination process, it will be varied of their progenies. This study aims to reveal the genetic variation of sago palms progenies with naturally pollinated process. The research method is to collect seeds from one parent trees that have produced ripe fruit. Fruit seeds germinated to be made and tested genetic variation using RAPD markers. Isolation of DNA is done by using the fresh young leaves. DNA amplification is done by using RAPD primers. The results showed that the progenies derived from naturally pollinated of sago palms were genetically varied based on RAPD markers and also varied based on morphological phenotypic. Variations occurred in the progenies of sago palm indicated that the sago palms were estimated cross-pollinated naturally, as a result fruits and seeds with genetically differences.
文摘Orchidaceae has known as an attractive flower and immense species. We have found a large species of Orchidaceae grow naturally in Papua’s jungle, Indonesia territorial. This study aims to reveal genetic variation and genetic relationships among endemic orchids in Papua based on RAPD markers. The study included 26 accessions of Papua’s endemic orchids used for genomic DNA extraction. Genomic DNAs were extracted by usingDNAextraction kit from Qiagen and genomic DNA amplification by using 10 decamer RAPD primers. DNA fragments that were amplified by Polymerase Change Reaction (PCR) were visualized and documented by using UV illumination apparatus. Genetically, endemic Orchids in Papua were described high variation. Fragments amplification by using ten RAPD primers and performed in the PCR tools resulted in 54 numbers of polymorphic fragments and no monomorphic band. The number of polymorphic bands per primer ranged from 4 to 7 with averaged 5.4 bands per assay unit. The genetic dissimilarities (GDs) among examined orchids ranged from 0.10 to 0.94 based on Nei’s unbiased coefficients. Dendrogram construction showed that Papua’s endemic orchid (PEO) samples different from another and separated to form group by their own at the 0.40 coefficient value and at the 0.6 coefficient value indicate that PEO sample is divided into nine groups i.e. samples at the genera level were separated into their own groups.
文摘Papua is rich in biodiversity. The indigenous Papuans utilize most of endemic plants as food, board and medicines. One of the plants used as medicine is La-portea, sp, that locally known as Daun Gatal. The plant however, has not been developed commercially;this might due to lack of scientific providence of medicine content in this plant. The aims of this research is to (1) explore the types of Laportea growing in Papua, (2) conduct bio molecular character, which observes phylogeny relationship or genetic morphology in order to obtain data on the number of species found in Manokwari and Nabire. Bio molecular analysis is performed by PCR. Genetic analysis in this study was conducted through several phases, as follow: (a) Isolation of genomic total, (b) amplification of genes that encoded Rbcl, (c) sequencing the nucleotide sequence of the gene Rbcl of Laportea, (d) phylogeny analysis. The results showed that the DNA quality was good enough to do the next analysis. Of the four samples, two obtained haplo types are Haplo Type 1 (DT.NBX.H, DT.NBX.U and DR.MKW.H) and Haplo Type 2 (DR.NBX.L). The results of the analysis of phylogeny trees showed samples at 1 in the same haplo type, clade sequences of the genus Dendrocnide spp, Discocnide, and Laportea, while the sample at 2 in the same haplotype clade with Laportea interupta, Laportea ruderalis and Urera sp.
